Sevoflurane suppresses migration and invasion of glioma cells by regulating miR-146b-5p and MMP16
Background Glioma is the most common brain tumor with poor prognosis all over the world. Anesthetics have been demonstrated to have important impacts on cell migration and invasion in different cancers. However, the underlying mechanism that allows anesthetics-mediated progression of glioma cells re...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-12-01
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| Series: | Artificial Cells, Nanomedicine, and Biotechnology |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/21691401.2019.1648282 |
| _version_ | 1811291906873753600 |
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| author | Le Zhang Jun Wang Zhijie Fu YanQiu Ai Yanrong Li Ying Wang Yanping Wang |
| author_facet | Le Zhang Jun Wang Zhijie Fu YanQiu Ai Yanrong Li Ying Wang Yanping Wang |
| author_sort | Le Zhang |
| collection | DOAJ |
| description | Background Glioma is the most common brain tumor with poor prognosis all over the world. Anesthetics have been demonstrated to have important impacts on cell migration and invasion in different cancers. However, the underlying mechanism that allows anesthetics-mediated progression of glioma cells remains elusive.Methods Sevoflurane (Sev), a class of common anesthetics, was used to expose to U87-MG and U251 cells. The expressions of microRNA-146b-5p (miR-146b-5p) and matrix metallopeptidase 16 (MMP16)were measured by quantitative real-time polymerase chain reaction or western blot. Transfection was performed in glioma cells with miR-146b-5p inhibitor, inhibitor negative control, MMP16 overexpression vector, empty vector, small interfering RNA against MMP16 or scramble. Cell migration and invasion were analyzed by the trans-well assay. The interaction between miR-146b-5p and MMP16 was explored by luciferase activity and RNA immunoprecipitation assays.Results Sev treatment inhibited migration and invasion of glioma cells. The expression of miR-146b-5p was enhanced and MMP16 protein was decreased in glioma cells after exposure of Sev. Knockdown of miR-146b-5p or overexpression of MMP16 reversed Sev-induced inhibition of migration and invasion of glioma cells. Moreover, MMP16 was indicated as a target of miR-146b-5p and its silencing attenuated the regulatory role of miR-146b-5p abrogationin Sev-treated glioma cells.Conclusion Sev impeded cell migration and invasion through regulating miR-146b-5p and MMP16 in glioma, indicating a novel theories foundation for the application of anesthetics like Sev in glioma. |
| first_indexed | 2024-04-13T04:36:26Z |
| format | Article |
| id | doaj.art-efd6b0a5a6444daf8795e925757f129d |
| institution | Directory Open Access Journal |
| issn | 2169-1401 2169-141X |
| language | English |
| last_indexed | 2024-04-13T04:36:26Z |
| publishDate | 2019-12-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Artificial Cells, Nanomedicine, and Biotechnology |
| spelling | doaj.art-efd6b0a5a6444daf8795e925757f129d2022-12-22T03:02:09ZengTaylor & Francis GroupArtificial Cells, Nanomedicine, and Biotechnology2169-14012169-141X2019-12-014713306331410.1080/21691401.2019.1648282Sevoflurane suppresses migration and invasion of glioma cells by regulating miR-146b-5p and MMP16Le Zhang0Jun Wang1Zhijie Fu2YanQiu Ai3Yanrong Li4Ying Wang5Yanping Wang6Department of Anesthesiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Operating Room, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Anesthesiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Anesthesiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Anesthesiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Anesthesiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaDepartment of Anesthesiology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, ChinaBackground Glioma is the most common brain tumor with poor prognosis all over the world. Anesthetics have been demonstrated to have important impacts on cell migration and invasion in different cancers. However, the underlying mechanism that allows anesthetics-mediated progression of glioma cells remains elusive.Methods Sevoflurane (Sev), a class of common anesthetics, was used to expose to U87-MG and U251 cells. The expressions of microRNA-146b-5p (miR-146b-5p) and matrix metallopeptidase 16 (MMP16)were measured by quantitative real-time polymerase chain reaction or western blot. Transfection was performed in glioma cells with miR-146b-5p inhibitor, inhibitor negative control, MMP16 overexpression vector, empty vector, small interfering RNA against MMP16 or scramble. Cell migration and invasion were analyzed by the trans-well assay. The interaction between miR-146b-5p and MMP16 was explored by luciferase activity and RNA immunoprecipitation assays.Results Sev treatment inhibited migration and invasion of glioma cells. The expression of miR-146b-5p was enhanced and MMP16 protein was decreased in glioma cells after exposure of Sev. Knockdown of miR-146b-5p or overexpression of MMP16 reversed Sev-induced inhibition of migration and invasion of glioma cells. Moreover, MMP16 was indicated as a target of miR-146b-5p and its silencing attenuated the regulatory role of miR-146b-5p abrogationin Sev-treated glioma cells.Conclusion Sev impeded cell migration and invasion through regulating miR-146b-5p and MMP16 in glioma, indicating a novel theories foundation for the application of anesthetics like Sev in glioma.https://www.tandfonline.com/doi/10.1080/21691401.2019.1648282GliomaanestheticsmiR-146b-5pMMP16migrationinvasion |
| spellingShingle | Le Zhang Jun Wang Zhijie Fu YanQiu Ai Yanrong Li Ying Wang Yanping Wang Sevoflurane suppresses migration and invasion of glioma cells by regulating miR-146b-5p and MMP16 Artificial Cells, Nanomedicine, and Biotechnology Glioma anesthetics miR-146b-5p MMP16 migration invasion |
| title | Sevoflurane suppresses migration and invasion of glioma cells by regulating miR-146b-5p and MMP16 |
| title_full | Sevoflurane suppresses migration and invasion of glioma cells by regulating miR-146b-5p and MMP16 |
| title_fullStr | Sevoflurane suppresses migration and invasion of glioma cells by regulating miR-146b-5p and MMP16 |
| title_full_unstemmed | Sevoflurane suppresses migration and invasion of glioma cells by regulating miR-146b-5p and MMP16 |
| title_short | Sevoflurane suppresses migration and invasion of glioma cells by regulating miR-146b-5p and MMP16 |
| title_sort | sevoflurane suppresses migration and invasion of glioma cells by regulating mir 146b 5p and mmp16 |
| topic | Glioma anesthetics miR-146b-5p MMP16 migration invasion |
| url | https://www.tandfonline.com/doi/10.1080/21691401.2019.1648282 |
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