<b>Permeabilization of Saccharomyces fragilis IZ 275 cells with ethanol to obtain a biocatalyst with lactose hydrolysis capacity

<b>Permeabilization of Saccharomyces fragilis IZ 275 cells with ethanol to obtain a biocatalyst with lactose hydrolysis capacity

The permeabilization was used to transform microorganisms in cell biocatalysts with high enzymatic activity. The Saccharomyces fragilis IZ 275 yeast cells were permeabilized with ethanol, as permeabilizing agent. To optimize the permeabilization conditions were used the design of Box-Behnken 15 tria...

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Bibliographic Details
Main Authors: Luiz Rodrigo Ito Morioka, Geyci de Oliveira Colognesi, Hélio Hiroshi Suguimoto
Format: Article
Language:English
Published: Universidade Estadual de Maringá 2016-10-01
Series:Acta Scientiarum: Biological Sciences
Subjects:
permeabilizing agent
beta-galactosidase
microbial biotechnology
optimal conditions
Online Access:https://www.periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/29220

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