Cancer radioresistance is characterized by a differential lipid droplet content along the cell cycle
Abstract Background Cancer radiation treatments have seen substantial advancements, yet the biomolecular mechanisms underlying cancer cell radioresistance continue to elude full understanding. The effectiveness of radiation on cancer is hindered by various factors, such as oxygen concentrations with...
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| Format: | Članak |
| Jezik: | English |
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BMC
2024-04-01
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| Serija: | Cell Division |
| Teme: | |
| Online pristup: | https://doi.org/10.1186/s13008-024-00116-y |
| _version_ | 1827277066001711104 |
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| author | Francesca Pagliari Jeannette Jansen Jan Knoll Rachel Hanley Joao Seco Luca Tirinato |
| author_facet | Francesca Pagliari Jeannette Jansen Jan Knoll Rachel Hanley Joao Seco Luca Tirinato |
| author_sort | Francesca Pagliari |
| collection | DOAJ |
| description | Abstract Background Cancer radiation treatments have seen substantial advancements, yet the biomolecular mechanisms underlying cancer cell radioresistance continue to elude full understanding. The effectiveness of radiation on cancer is hindered by various factors, such as oxygen concentrations within tumors, cells’ ability to repair DNA damage and metabolic changes. Moreover, the initial and radiation-induced cell cycle profiles can significantly influence radiotherapy responses as radiation sensitivity fluctuates across different cell cycle stages. Given this evidence and our prior studies establishing a correlation between cancer radiation resistance and an increased number of cytoplasmic Lipid Droplets (LDs), we investigated if LD accumulation was modulated along the cell cycle and if this correlated with differential radioresistance in lung and bladder cell lines. Results Our findings identified the S phase as the most radioresistant cell cycle phase being characterized by an increase in LDs. Analysis of the expression of perilipin genes (a family of proteins involved in the LD structure and functions) throughout the cell cycle also uncovered a unique gene cell cycle pattern. Conclusions In summary, although these results require further molecular studies about the mechanisms of radioresistance, the findings presented here are the first evidence that LD accumulation could participate in cancer cells’ ability to better survive X-Ray radiation when cells are in the S phase. LDs can represent new players in the radioresistance processes associated with cancer metabolism. This could open new therapeutic avenues in which the use of LD-interfering drugs might enhance cancer sensitivity to radiation. |
| first_indexed | 2024-04-24T07:14:39Z |
| format | Article |
| id | doaj.art-efe3054bdb9544969264392f1ee8c14e |
| institution | Directory Open Access Journal |
| issn | 1747-1028 |
| language | English |
| last_indexed | 2024-04-24T07:14:39Z |
| publishDate | 2024-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Cell Division |
| spelling | doaj.art-efe3054bdb9544969264392f1ee8c14e2024-04-21T11:24:52ZengBMCCell Division1747-10282024-04-011911810.1186/s13008-024-00116-yCancer radioresistance is characterized by a differential lipid droplet content along the cell cycleFrancesca Pagliari0Jeannette Jansen1Jan Knoll2Rachel Hanley3Joao Seco4Luca Tirinato5Division of Biomedical Physics in Radiation Oncology, German Cancer Research Center (DKFZ)Division of Biomedical Physics in Radiation Oncology, German Cancer Research Center (DKFZ)Division of Biomedical Physics in Radiation Oncology, German Cancer Research Center (DKFZ)Division of Biomedical Physics in Radiation Oncology, German Cancer Research Center (DKFZ)Division of Biomedical Physics in Radiation Oncology, German Cancer Research Center (DKFZ)Division of Biomedical Physics in Radiation Oncology, German Cancer Research Center (DKFZ)Abstract Background Cancer radiation treatments have seen substantial advancements, yet the biomolecular mechanisms underlying cancer cell radioresistance continue to elude full understanding. The effectiveness of radiation on cancer is hindered by various factors, such as oxygen concentrations within tumors, cells’ ability to repair DNA damage and metabolic changes. Moreover, the initial and radiation-induced cell cycle profiles can significantly influence radiotherapy responses as radiation sensitivity fluctuates across different cell cycle stages. Given this evidence and our prior studies establishing a correlation between cancer radiation resistance and an increased number of cytoplasmic Lipid Droplets (LDs), we investigated if LD accumulation was modulated along the cell cycle and if this correlated with differential radioresistance in lung and bladder cell lines. Results Our findings identified the S phase as the most radioresistant cell cycle phase being characterized by an increase in LDs. Analysis of the expression of perilipin genes (a family of proteins involved in the LD structure and functions) throughout the cell cycle also uncovered a unique gene cell cycle pattern. Conclusions In summary, although these results require further molecular studies about the mechanisms of radioresistance, the findings presented here are the first evidence that LD accumulation could participate in cancer cells’ ability to better survive X-Ray radiation when cells are in the S phase. LDs can represent new players in the radioresistance processes associated with cancer metabolism. This could open new therapeutic avenues in which the use of LD-interfering drugs might enhance cancer sensitivity to radiation.https://doi.org/10.1186/s13008-024-00116-yLipid dropletsRadioresistanceCancer metabolismCell cyclePerilipins |
| spellingShingle | Francesca Pagliari Jeannette Jansen Jan Knoll Rachel Hanley Joao Seco Luca Tirinato Cancer radioresistance is characterized by a differential lipid droplet content along the cell cycle Cell Division Lipid droplets Radioresistance Cancer metabolism Cell cycle Perilipins |
| title | Cancer radioresistance is characterized by a differential lipid droplet content along the cell cycle |
| title_full | Cancer radioresistance is characterized by a differential lipid droplet content along the cell cycle |
| title_fullStr | Cancer radioresistance is characterized by a differential lipid droplet content along the cell cycle |
| title_full_unstemmed | Cancer radioresistance is characterized by a differential lipid droplet content along the cell cycle |
| title_short | Cancer radioresistance is characterized by a differential lipid droplet content along the cell cycle |
| title_sort | cancer radioresistance is characterized by a differential lipid droplet content along the cell cycle |
| topic | Lipid droplets Radioresistance Cancer metabolism Cell cycle Perilipins |
| url | https://doi.org/10.1186/s13008-024-00116-y |
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