Central Substance P Attenuates RF-amid-related Peptide-3 Impacts on the Serum Level of Luteinizing Hormone in Wistar Rats
Introduction: It is well-established that gonadotropin-inhibitory hormone (GnIH) and its mammalian orthologues (RFRP: RF-amid related peptides) can decrease gonadotrophin secretion. Moreover, substance P (SP) is another modulator of the secretion of gonadotropins in a species-dependent manner. This...
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| Format: | Article |
| Language: | English |
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Zabol University of Medical sciences
2019-03-01
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| Series: | International Journal of Basic Science in Medicine |
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| Online Access: | http://ijbsm.zbmu.ac.ir/PDF/ijbsm-3255 |
| _version_ | 1811325867078451200 |
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| author | Parastoo Rahdar Homayoun Khazali |
| author_facet | Parastoo Rahdar Homayoun Khazali |
| author_sort | Parastoo Rahdar |
| collection | DOAJ |
| description | Introduction: It is well-established that gonadotropin-inhibitory hormone (GnIH) and its mammalian orthologues (RFRP: RF-amid related peptides) can decrease gonadotrophin secretion. Moreover, substance P (SP) is another modulator of the secretion of gonadotropins in a species-dependent manner. This study aimed to find out the impacts of concomitant infusion of RFRP-3 and SP on luteinizing hormone (LH) concentration. Methods: Forty-two rats were arbitrarily assigned to 7 groups (n=6 per group). Animals in the experimental groups were intracerebroventricularly injected with saline +DMSO, SP, RFRP-3, SP + RFRP-3, SP + RF9 (RFRP-3 receptor antagonist), SP + P234 (kisspeptin receptor antagonist) + RFRP-3 and SP + CP-96,345 (SP receptor antagonist) + RFRP-3 in a final volume of 3 µL. Blood samples were collected at 30-minute intervals after injections, and serum was used to measure the LH concentration by radioimmunoassay. Results: According to the results, injections led to the elevation of LH serum concentration at 30-minute post injection (P<0.05) in the SP and SP+RF9 groups. Moreover, administration of either RFRP-3 or SP + RFRP-3 + SP receptor antagonist strikingly decreased the LH mean serum concentration at 30-minute after injections (P<0.05). On the contrary, the infusion of SP+RFRP-3 and SP+RFRP-3+P234 caused no dramatic changes in the LH mean serum concentration. Conclusion: In general, the data showed that SP suppresses the impacts of RFRP-3 on the serum levels of LH. |
| first_indexed | 2024-04-13T14:41:04Z |
| format | Article |
| id | doaj.art-efe65d2e237d48cd82b2c91003205d6f |
| institution | Directory Open Access Journal |
| issn | 2476-664X |
| language | English |
| last_indexed | 2024-04-13T14:41:04Z |
| publishDate | 2019-03-01 |
| publisher | Zabol University of Medical sciences |
| record_format | Article |
| series | International Journal of Basic Science in Medicine |
| spelling | doaj.art-efe65d2e237d48cd82b2c91003205d6f2022-12-22T02:42:54ZengZabol University of Medical sciencesInternational Journal of Basic Science in Medicine2476-664X2019-03-01414910.15171/ijbsm.2019.02ijbsm-3255Central Substance P Attenuates RF-amid-related Peptide-3 Impacts on the Serum Level of Luteinizing Hormone in Wistar RatsParastoo Rahdar0Homayoun Khazali1Department of Animal Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, IranDepartment of Animal Sciences and Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, IranIntroduction: It is well-established that gonadotropin-inhibitory hormone (GnIH) and its mammalian orthologues (RFRP: RF-amid related peptides) can decrease gonadotrophin secretion. Moreover, substance P (SP) is another modulator of the secretion of gonadotropins in a species-dependent manner. This study aimed to find out the impacts of concomitant infusion of RFRP-3 and SP on luteinizing hormone (LH) concentration. Methods: Forty-two rats were arbitrarily assigned to 7 groups (n=6 per group). Animals in the experimental groups were intracerebroventricularly injected with saline +DMSO, SP, RFRP-3, SP + RFRP-3, SP + RF9 (RFRP-3 receptor antagonist), SP + P234 (kisspeptin receptor antagonist) + RFRP-3 and SP + CP-96,345 (SP receptor antagonist) + RFRP-3 in a final volume of 3 µL. Blood samples were collected at 30-minute intervals after injections, and serum was used to measure the LH concentration by radioimmunoassay. Results: According to the results, injections led to the elevation of LH serum concentration at 30-minute post injection (P<0.05) in the SP and SP+RF9 groups. Moreover, administration of either RFRP-3 or SP + RFRP-3 + SP receptor antagonist strikingly decreased the LH mean serum concentration at 30-minute after injections (P<0.05). On the contrary, the infusion of SP+RFRP-3 and SP+RFRP-3+P234 caused no dramatic changes in the LH mean serum concentration. Conclusion: In general, the data showed that SP suppresses the impacts of RFRP-3 on the serum levels of LH.http://ijbsm.zbmu.ac.ir/PDF/ijbsm-3255Substance pRF-amid-related peptide-3GnRHLuteinizing hormoneKisspeptinRat |
| spellingShingle | Parastoo Rahdar Homayoun Khazali Central Substance P Attenuates RF-amid-related Peptide-3 Impacts on the Serum Level of Luteinizing Hormone in Wistar Rats International Journal of Basic Science in Medicine Substance p RF-amid-related peptide-3 GnRH Luteinizing hormone Kisspeptin Rat |
| title | Central Substance P Attenuates RF-amid-related Peptide-3 Impacts on the Serum Level of Luteinizing Hormone in Wistar Rats |
| title_full | Central Substance P Attenuates RF-amid-related Peptide-3 Impacts on the Serum Level of Luteinizing Hormone in Wistar Rats |
| title_fullStr | Central Substance P Attenuates RF-amid-related Peptide-3 Impacts on the Serum Level of Luteinizing Hormone in Wistar Rats |
| title_full_unstemmed | Central Substance P Attenuates RF-amid-related Peptide-3 Impacts on the Serum Level of Luteinizing Hormone in Wistar Rats |
| title_short | Central Substance P Attenuates RF-amid-related Peptide-3 Impacts on the Serum Level of Luteinizing Hormone in Wistar Rats |
| title_sort | central substance p attenuates rf amid related peptide 3 impacts on the serum level of luteinizing hormone in wistar rats |
| topic | Substance p RF-amid-related peptide-3 GnRH Luteinizing hormone Kisspeptin Rat |
| url | http://ijbsm.zbmu.ac.ir/PDF/ijbsm-3255 |
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