Kinase-Based Screening of Marine Natural Extracts Leads to the Identification of a Cytotoxic High Molecular Weight Metabolite from the Mediterranean Sponge <i>Crambe tailliezi</i>
Regulated cell death (RCD) results from the activation of one or more signal transduction modules both in physiological or pathological conditions. It is now established that RCD is involved in numerous human diseases, including cancer. As regulated cell death processes can be modulated by pharmacol...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-10-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/17/10/569 |
| _version_ | 1798041434896465920 |
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| author | Thi-Ngoc-Dung Nguyen Omid Feizbakhsh Estelle Sfecci Blandine Baratte Claire Delehouzé Adrien Garcia Corentin Moulin Pierre Colas Sandrine Ruchaud Mohamed Mehiri Stéphane Bach |
| author_facet | Thi-Ngoc-Dung Nguyen Omid Feizbakhsh Estelle Sfecci Blandine Baratte Claire Delehouzé Adrien Garcia Corentin Moulin Pierre Colas Sandrine Ruchaud Mohamed Mehiri Stéphane Bach |
| author_sort | Thi-Ngoc-Dung Nguyen |
| collection | DOAJ |
| description | Regulated cell death (RCD) results from the activation of one or more signal transduction modules both in physiological or pathological conditions. It is now established that RCD is involved in numerous human diseases, including cancer. As regulated cell death processes can be modulated by pharmacological tools, the research reported here aims to characterize new marine compounds acting as RCD modulators. Protein kinases (PKs) are key signaling actors in various RCDs notably through the control of either mitosis (e.g., the PKs Aurora A and B) or necroptosis (e.g., RIPK1 and RIPK3). From the primary screening of 27 various extracts of marine organisms collected in the Mediterranean Sea, an extract and subsequently a purified high molecular weight compound dubbed P3, were isolated from the marine sponge <i>Crambe tailliezi</i> and characterized as a selective inhibitor of PKs Aurora A and B. Furthermore, P3 was shown to induce apoptosis and to decrease proliferation and mitotic index of human osteosarcoma U-2 OS cells. |
| first_indexed | 2024-04-11T22:21:29Z |
| format | Article |
| id | doaj.art-efe9d9089528481dabd378f52d0fc097 |
| institution | Directory Open Access Journal |
| issn | 1660-3397 |
| language | English |
| last_indexed | 2024-04-11T22:21:29Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Marine Drugs |
| spelling | doaj.art-efe9d9089528481dabd378f52d0fc0972022-12-22T04:00:08ZengMDPI AGMarine Drugs1660-33972019-10-01171056910.3390/md17100569md17100569Kinase-Based Screening of Marine Natural Extracts Leads to the Identification of a Cytotoxic High Molecular Weight Metabolite from the Mediterranean Sponge <i>Crambe tailliezi</i>Thi-Ngoc-Dung Nguyen0Omid Feizbakhsh1Estelle Sfecci2Blandine Baratte3Claire Delehouzé4Adrien Garcia5Corentin Moulin6Pierre Colas7Sandrine Ruchaud8Mohamed Mehiri9Stéphane Bach10Integrative Biology of Marine Models Laboratory (LBI2M), Sorbonne Université, CNRS, UMR 8227, Station Biologique de Roscoff, CS 90074, 29688 Roscoff Cedex, FranceIntegrative Biology of Marine Models Laboratory (LBI2M), Sorbonne Université, CNRS, UMR 8227, Station Biologique de Roscoff, CS 90074, 29688 Roscoff Cedex, FranceMarine Natural Products Team, Université Côte d’Azur, CNRS, Institut de Chimie de Nice, UMR 7272, 06108 Nice, FranceIntegrative Biology of Marine Models Laboratory (LBI2M), Sorbonne Université, CNRS, UMR 8227, Station Biologique de Roscoff, CS 90074, 29688 Roscoff Cedex, FranceIntegrative Biology of Marine Models Laboratory (LBI2M), Sorbonne Université, CNRS, UMR 8227, Station Biologique de Roscoff, CS 90074, 29688 Roscoff Cedex, FranceMarine Natural Products Team, Université Côte d’Azur, CNRS, Institut de Chimie de Nice, UMR 7272, 06108 Nice, FranceMarine Natural Products Team, Université Côte d’Azur, CNRS, Institut de Chimie de Nice, UMR 7272, 06108 Nice, FranceIntegrative Biology of Marine Models Laboratory (LBI2M), Sorbonne Université, CNRS, UMR 8227, Station Biologique de Roscoff, CS 90074, 29688 Roscoff Cedex, FranceIntegrative Biology of Marine Models Laboratory (LBI2M), Sorbonne Université, CNRS, UMR 8227, Station Biologique de Roscoff, CS 90074, 29688 Roscoff Cedex, FranceMarine Natural Products Team, Université Côte d’Azur, CNRS, Institut de Chimie de Nice, UMR 7272, 06108 Nice, FranceIntegrative Biology of Marine Models Laboratory (LBI2M), Sorbonne Université, CNRS, UMR 8227, Station Biologique de Roscoff, CS 90074, 29688 Roscoff Cedex, FranceRegulated cell death (RCD) results from the activation of one or more signal transduction modules both in physiological or pathological conditions. It is now established that RCD is involved in numerous human diseases, including cancer. As regulated cell death processes can be modulated by pharmacological tools, the research reported here aims to characterize new marine compounds acting as RCD modulators. Protein kinases (PKs) are key signaling actors in various RCDs notably through the control of either mitosis (e.g., the PKs Aurora A and B) or necroptosis (e.g., RIPK1 and RIPK3). From the primary screening of 27 various extracts of marine organisms collected in the Mediterranean Sea, an extract and subsequently a purified high molecular weight compound dubbed P3, were isolated from the marine sponge <i>Crambe tailliezi</i> and characterized as a selective inhibitor of PKs Aurora A and B. Furthermore, P3 was shown to induce apoptosis and to decrease proliferation and mitotic index of human osteosarcoma U-2 OS cells.https://www.mdpi.com/1660-3397/17/10/569screeningkinase inhibitionregulated cell deathrip1-independent cell deathmarine natural products<i>crambe tailliezi</i> |
| spellingShingle | Thi-Ngoc-Dung Nguyen Omid Feizbakhsh Estelle Sfecci Blandine Baratte Claire Delehouzé Adrien Garcia Corentin Moulin Pierre Colas Sandrine Ruchaud Mohamed Mehiri Stéphane Bach Kinase-Based Screening of Marine Natural Extracts Leads to the Identification of a Cytotoxic High Molecular Weight Metabolite from the Mediterranean Sponge <i>Crambe tailliezi</i> Marine Drugs screening kinase inhibition regulated cell death rip1-independent cell death marine natural products <i>crambe tailliezi</i> |
| title | Kinase-Based Screening of Marine Natural Extracts Leads to the Identification of a Cytotoxic High Molecular Weight Metabolite from the Mediterranean Sponge <i>Crambe tailliezi</i> |
| title_full | Kinase-Based Screening of Marine Natural Extracts Leads to the Identification of a Cytotoxic High Molecular Weight Metabolite from the Mediterranean Sponge <i>Crambe tailliezi</i> |
| title_fullStr | Kinase-Based Screening of Marine Natural Extracts Leads to the Identification of a Cytotoxic High Molecular Weight Metabolite from the Mediterranean Sponge <i>Crambe tailliezi</i> |
| title_full_unstemmed | Kinase-Based Screening of Marine Natural Extracts Leads to the Identification of a Cytotoxic High Molecular Weight Metabolite from the Mediterranean Sponge <i>Crambe tailliezi</i> |
| title_short | Kinase-Based Screening of Marine Natural Extracts Leads to the Identification of a Cytotoxic High Molecular Weight Metabolite from the Mediterranean Sponge <i>Crambe tailliezi</i> |
| title_sort | kinase based screening of marine natural extracts leads to the identification of a cytotoxic high molecular weight metabolite from the mediterranean sponge i crambe tailliezi i |
| topic | screening kinase inhibition regulated cell death rip1-independent cell death marine natural products <i>crambe tailliezi</i> |
| url | https://www.mdpi.com/1660-3397/17/10/569 |
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