Optimization of bacterioruberin production from Halorubrum ruber and assessment of its antioxidant potential
Abstract Haloarchaea produce bacterioruberin, a major C50 carotenoid with antioxidant properties that allow for its potential application in the food, cosmetic, and pharmaceutical industries. This study aimed to optimize culture conditions for total carotenoid, predominantly comprising bacterioruber...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2024-01-01
|
| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12934-023-02274-0 |
| _version_ | 1797362749070639104 |
|---|---|
| author | Chi Young Hwang Eui-Sang Cho Sungjun Kim Kyobum Kim Myung-Ji Seo |
| author_facet | Chi Young Hwang Eui-Sang Cho Sungjun Kim Kyobum Kim Myung-Ji Seo |
| author_sort | Chi Young Hwang |
| collection | DOAJ |
| description | Abstract Haloarchaea produce bacterioruberin, a major C50 carotenoid with antioxidant properties that allow for its potential application in the food, cosmetic, and pharmaceutical industries. This study aimed to optimize culture conditions for total carotenoid, predominantly comprising bacterioruberin, production using Halorubrum ruber MBLA0099. A one-factor-at-a-time and statistically-based experimental design were applied to optimize the culture conditions. Culture in the optimized medium caused an increase in total carotenoid production from 0.496 to 1.966 mg L− 1 Maximal carotenoid productivity was achieved in a 7-L laboratory-scale fermentation and represented a 6.05-fold increase (0.492 mg L–1 d–1). The carotenoid extracts from strain MBLA0099 exhibited a 1.8–10.3-fold higher antioxidant activity in vitro, and allowed for a higher survival rate of Caenorhabditis elegans under oxidative stress conditions. These results demonstrated that Hrr. ruber MBLA0099 has significant potential as a haloarchaon for the commercial production of bacterioruberin. |
| first_indexed | 2024-03-08T16:11:55Z |
| format | Article |
| id | doaj.art-efea265c525f418fb81d9905b183b87f |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-03-08T16:11:55Z |
| publishDate | 2024-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-efea265c525f418fb81d9905b183b87f2024-01-07T12:55:39ZengBMCMicrobial Cell Factories1475-28592024-01-0123111910.1186/s12934-023-02274-0Optimization of bacterioruberin production from Halorubrum ruber and assessment of its antioxidant potentialChi Young Hwang0Eui-Sang Cho1Sungjun Kim2Kyobum Kim3Myung-Ji Seo4Department of Bioengineering and Nano-Bioengineering, Graduate School of Incheon National UniversityDepartment of Bioengineering and Nano-Bioengineering, Graduate School of Incheon National UniversityDepartment of Chemical and Biochemical Engineering, Dongguk UniversityDepartment of Chemical and Biochemical Engineering, Dongguk UniversityDepartment of Bioengineering and Nano-Bioengineering, Graduate School of Incheon National UniversityAbstract Haloarchaea produce bacterioruberin, a major C50 carotenoid with antioxidant properties that allow for its potential application in the food, cosmetic, and pharmaceutical industries. This study aimed to optimize culture conditions for total carotenoid, predominantly comprising bacterioruberin, production using Halorubrum ruber MBLA0099. A one-factor-at-a-time and statistically-based experimental design were applied to optimize the culture conditions. Culture in the optimized medium caused an increase in total carotenoid production from 0.496 to 1.966 mg L− 1 Maximal carotenoid productivity was achieved in a 7-L laboratory-scale fermentation and represented a 6.05-fold increase (0.492 mg L–1 d–1). The carotenoid extracts from strain MBLA0099 exhibited a 1.8–10.3-fold higher antioxidant activity in vitro, and allowed for a higher survival rate of Caenorhabditis elegans under oxidative stress conditions. These results demonstrated that Hrr. ruber MBLA0099 has significant potential as a haloarchaon for the commercial production of bacterioruberin.https://doi.org/10.1186/s12934-023-02274-0BacterioruberinHalorubrum ruberCarotenoidOptimizationAntioxidant |
| spellingShingle | Chi Young Hwang Eui-Sang Cho Sungjun Kim Kyobum Kim Myung-Ji Seo Optimization of bacterioruberin production from Halorubrum ruber and assessment of its antioxidant potential Microbial Cell Factories Bacterioruberin Halorubrum ruber Carotenoid Optimization Antioxidant |
| title | Optimization of bacterioruberin production from Halorubrum ruber and assessment of its antioxidant potential |
| title_full | Optimization of bacterioruberin production from Halorubrum ruber and assessment of its antioxidant potential |
| title_fullStr | Optimization of bacterioruberin production from Halorubrum ruber and assessment of its antioxidant potential |
| title_full_unstemmed | Optimization of bacterioruberin production from Halorubrum ruber and assessment of its antioxidant potential |
| title_short | Optimization of bacterioruberin production from Halorubrum ruber and assessment of its antioxidant potential |
| title_sort | optimization of bacterioruberin production from halorubrum ruber and assessment of its antioxidant potential |
| topic | Bacterioruberin Halorubrum ruber Carotenoid Optimization Antioxidant |
| url | https://doi.org/10.1186/s12934-023-02274-0 |
| work_keys_str_mv | AT chiyounghwang optimizationofbacterioruberinproductionfromhalorubrumruberandassessmentofitsantioxidantpotential AT euisangcho optimizationofbacterioruberinproductionfromhalorubrumruberandassessmentofitsantioxidantpotential AT sungjunkim optimizationofbacterioruberinproductionfromhalorubrumruberandassessmentofitsantioxidantpotential AT kyobumkim optimizationofbacterioruberinproductionfromhalorubrumruberandassessmentofitsantioxidantpotential AT myungjiseo optimizationofbacterioruberinproductionfromhalorubrumruberandassessmentofitsantioxidantpotential |