In situ RT-PCR Optimized for Electron Microscopy Allows Description of Subcellular Morphology of Target mRNA-Expressing Cells in the Brain

In situ RT-PCR Optimized for Electron Microscopy Allows Description of Subcellular Morphology of Target mRNA-Expressing Cells in the Brain

In situ RT-PCR detects and amplifies mRNA (cDNA) while obtaining spatial information of gene expression. When the intended use is an ultrastructural analysis of morphology, the procedure may be technically challenging and quality of tissue dramatically altered by proteolytic digestion and extreme as...

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Bibliographic Details
Main Authors: Laura Cubas-Nuñez, María Duran-Moreno, Jessica Castillo-Villalba, Jorge Fuentes-Maestre, Bonaventura Casanova, José M. García-Verdugo, Sara Gil-Perotín
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-05-01
Series:Frontiers in Cellular Neuroscience
Subjects:
gene expression
brain
electron microscopy
immunogold
epoxy resin
pre-embedding
Online Access:http://journal.frontiersin.org/article/10.3389/fncel.2017.00141/full

Internet

http://journal.frontiersin.org/article/10.3389/fncel.2017.00141/full

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