Hedgehog Promotes Production of Inhibitory Interneurons in Vivo and in Vitro from Pluripotent Stem Cells
Loss or damage of cortical inhibitory interneurons characterizes a number of neurological disorders. There is therefore a great deal of interest in learning how to generate these neurons from a pluripotent stem cell source so they can be used for cell replacement therapies or for in vitro drug testi...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2016-08-01
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| Series: | Journal of Developmental Biology |
| Subjects: | |
| Online Access: | http://www.mdpi.com/2221-3759/4/3/26 |
| _version_ | 1818198492367028224 |
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| author | Nickesha C. Anderson Christopher Y. Chen Laura Grabel |
| author_facet | Nickesha C. Anderson Christopher Y. Chen Laura Grabel |
| author_sort | Nickesha C. Anderson |
| collection | DOAJ |
| description | Loss or damage of cortical inhibitory interneurons characterizes a number of neurological disorders. There is therefore a great deal of interest in learning how to generate these neurons from a pluripotent stem cell source so they can be used for cell replacement therapies or for in vitro drug testing. To design a directed differentiation protocol, a number of groups have used the information gained in the last 15 years detailing the conditions that promote interneuron progenitor differentiation in the ventral telencephalon during embryogenesis. The use of Hedgehog peptides and agonists is featured prominently in these approaches. We review here the data documenting a role for Hedgehog in specifying interneurons in both the embryonic brain during development and in vitro during the directed differentiation of pluripotent stem cells. |
| first_indexed | 2024-12-12T02:06:44Z |
| format | Article |
| id | doaj.art-efec5d408ee7498fb78e41f57cee535f |
| institution | Directory Open Access Journal |
| issn | 2221-3759 |
| language | English |
| last_indexed | 2024-12-12T02:06:44Z |
| publishDate | 2016-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Developmental Biology |
| spelling | doaj.art-efec5d408ee7498fb78e41f57cee535f2022-12-22T00:42:02ZengMDPI AGJournal of Developmental Biology2221-37592016-08-01432610.3390/jdb4030026jdb4030026Hedgehog Promotes Production of Inhibitory Interneurons in Vivo and in Vitro from Pluripotent Stem CellsNickesha C. Anderson0Christopher Y. Chen1Laura Grabel2Department of Biology, Wesleyan University, 52 Lawn Avenue, Middletown, CT 06459, USADepartment of Biology, Wesleyan University, 52 Lawn Avenue, Middletown, CT 06459, USADepartment of Biology, Wesleyan University, 52 Lawn Avenue, Middletown, CT 06459, USALoss or damage of cortical inhibitory interneurons characterizes a number of neurological disorders. There is therefore a great deal of interest in learning how to generate these neurons from a pluripotent stem cell source so they can be used for cell replacement therapies or for in vitro drug testing. To design a directed differentiation protocol, a number of groups have used the information gained in the last 15 years detailing the conditions that promote interneuron progenitor differentiation in the ventral telencephalon during embryogenesis. The use of Hedgehog peptides and agonists is featured prominently in these approaches. We review here the data documenting a role for Hedgehog in specifying interneurons in both the embryonic brain during development and in vitro during the directed differentiation of pluripotent stem cells.http://www.mdpi.com/2221-3759/4/3/26Sonic hedgehogGABAergic interneuronsmedial ganglionic eminencepluripotent stem cells |
| spellingShingle | Nickesha C. Anderson Christopher Y. Chen Laura Grabel Hedgehog Promotes Production of Inhibitory Interneurons in Vivo and in Vitro from Pluripotent Stem Cells Journal of Developmental Biology Sonic hedgehog GABAergic interneurons medial ganglionic eminence pluripotent stem cells |
| title | Hedgehog Promotes Production of Inhibitory Interneurons in Vivo and in Vitro from Pluripotent Stem Cells |
| title_full | Hedgehog Promotes Production of Inhibitory Interneurons in Vivo and in Vitro from Pluripotent Stem Cells |
| title_fullStr | Hedgehog Promotes Production of Inhibitory Interneurons in Vivo and in Vitro from Pluripotent Stem Cells |
| title_full_unstemmed | Hedgehog Promotes Production of Inhibitory Interneurons in Vivo and in Vitro from Pluripotent Stem Cells |
| title_short | Hedgehog Promotes Production of Inhibitory Interneurons in Vivo and in Vitro from Pluripotent Stem Cells |
| title_sort | hedgehog promotes production of inhibitory interneurons in vivo and in vitro from pluripotent stem cells |
| topic | Sonic hedgehog GABAergic interneurons medial ganglionic eminence pluripotent stem cells |
| url | http://www.mdpi.com/2221-3759/4/3/26 |
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