Singing from the grave: DNA from a 180 year old type specimen confirms the identity of Chrysoperla carnea (Stephens).
Historically serving as repositories for morphologically-based taxonomic research, natural history collections are now increasingly being targeted in studies utilizing DNA data. The development of advanced molecular techniques has facilitated extraction of useable DNA from old specimens, including t...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2015-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4390323?pdf=render |
| _version_ | 1818262390198763520 |
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| author | Ben W Price Charles S Henry Andie C Hall Atsushi Mochizuki Peter Duelli Stephen J Brooks |
| author_facet | Ben W Price Charles S Henry Andie C Hall Atsushi Mochizuki Peter Duelli Stephen J Brooks |
| author_sort | Ben W Price |
| collection | DOAJ |
| description | Historically serving as repositories for morphologically-based taxonomic research, natural history collections are now increasingly being targeted in studies utilizing DNA data. The development of advanced molecular techniques has facilitated extraction of useable DNA from old specimens, including type material. Sequencing diagnostic molecular markers from type material enables accurate species designation, especially where modern taxonomic hypotheses confirm morphologically cryptic species complexes. One such example is Chrysoperla carnea (Stephens), which belongs to a complex of about 20 cryptic species, most of which can only be reliably distinguished by their pre-mating courtship songs or by DNA analysis. The subtle morphological variation in the group has led to disagreement over the previous designation of the lectotype for C. carnea, an issue that has been further compounded because Chrysoperla carnea is a highly valued biological control agent in arable crops. Archival DNA extraction and sequencing from the 180 year old lectotype specimen, combined with Bayesian and Likelihood based phylogenetic analyses of modern specimens from the entire complex, were used to establish unambiguously the true identity of Chrysoperla carnea. |
| first_indexed | 2024-12-12T19:02:22Z |
| format | Article |
| id | doaj.art-efecb55395384223b37cfbbb8987afa5 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-12T19:02:22Z |
| publishDate | 2015-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-efecb55395384223b37cfbbb8987afa52022-12-22T00:15:03ZengPublic Library of Science (PLoS)PLoS ONE1932-62032015-01-01104e012112710.1371/journal.pone.0121127Singing from the grave: DNA from a 180 year old type specimen confirms the identity of Chrysoperla carnea (Stephens).Ben W PriceCharles S HenryAndie C HallAtsushi MochizukiPeter DuelliStephen J BrooksHistorically serving as repositories for morphologically-based taxonomic research, natural history collections are now increasingly being targeted in studies utilizing DNA data. The development of advanced molecular techniques has facilitated extraction of useable DNA from old specimens, including type material. Sequencing diagnostic molecular markers from type material enables accurate species designation, especially where modern taxonomic hypotheses confirm morphologically cryptic species complexes. One such example is Chrysoperla carnea (Stephens), which belongs to a complex of about 20 cryptic species, most of which can only be reliably distinguished by their pre-mating courtship songs or by DNA analysis. The subtle morphological variation in the group has led to disagreement over the previous designation of the lectotype for C. carnea, an issue that has been further compounded because Chrysoperla carnea is a highly valued biological control agent in arable crops. Archival DNA extraction and sequencing from the 180 year old lectotype specimen, combined with Bayesian and Likelihood based phylogenetic analyses of modern specimens from the entire complex, were used to establish unambiguously the true identity of Chrysoperla carnea.http://europepmc.org/articles/PMC4390323?pdf=render |
| spellingShingle | Ben W Price Charles S Henry Andie C Hall Atsushi Mochizuki Peter Duelli Stephen J Brooks Singing from the grave: DNA from a 180 year old type specimen confirms the identity of Chrysoperla carnea (Stephens). PLoS ONE |
| title | Singing from the grave: DNA from a 180 year old type specimen confirms the identity of Chrysoperla carnea (Stephens). |
| title_full | Singing from the grave: DNA from a 180 year old type specimen confirms the identity of Chrysoperla carnea (Stephens). |
| title_fullStr | Singing from the grave: DNA from a 180 year old type specimen confirms the identity of Chrysoperla carnea (Stephens). |
| title_full_unstemmed | Singing from the grave: DNA from a 180 year old type specimen confirms the identity of Chrysoperla carnea (Stephens). |
| title_short | Singing from the grave: DNA from a 180 year old type specimen confirms the identity of Chrysoperla carnea (Stephens). |
| title_sort | singing from the grave dna from a 180 year old type specimen confirms the identity of chrysoperla carnea stephens |
| url | http://europepmc.org/articles/PMC4390323?pdf=render |
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