Effect of Granotsit preparation on mesenchymal bone marrow stem cells in experimental model of secondary immune deficiency with the aid of cyclophosphan
Experiments with 60 male and female mice of Balb/c line with mass of 18—21 g have been carried out. All manipulations, including ether euthanasia, were performed within the framework of approved rules for experiments with experimental animals. The mice were divided into four groups each of 15 animal...
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| Language: | English |
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Siberian State Medical University (Tomsk)
2008-09-01
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| Series: | Бюллетень сибирской медицины |
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| Online Access: | https://bulletin.ssmu.ru/jour/article/view/3425 |
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| author | A. N. Baikov V. P. Shakhov N. Yu. Shelgaev V. A. Serebryakova |
| author_facet | A. N. Baikov V. P. Shakhov N. Yu. Shelgaev V. A. Serebryakova |
| author_sort | A. N. Baikov |
| collection | DOAJ |
| description | Experiments with 60 male and female mice of Balb/c line with mass of 18—21 g have been carried out. All manipulations, including ether euthanasia, were performed within the framework of approved rules for experiments with experimental animals. The mice were divided into four groups each of 15 animals. The groups were injected with physiological salt solution (first group), cycliphosphan (second group), Granotsit preparation (recombinant granulocytic colony-stimulating factor) (third group), and cycliphosphan in Granotsit preparation (fourth group). We have studied the total number of leucocytes in blood, bone marrow cellularity, content of granulocytic- macrophage colony-forming cells and mesenchyma l precursors with the aid of tissue culture in vitro. It has been found that the Granotsit preparation can stimulate myelopoiesis (direct specific action) and mesenchymopo iesis (indirect action) in norm. When a cytostatic agent, the studied cytokine has a protective action on both myeloid and mesenchyma l precursors of bone marrow. |
| first_indexed | 2024-04-10T01:12:32Z |
| format | Article |
| id | doaj.art-eff1090167c74dedb955c9e5c75b7ed2 |
| institution | Directory Open Access Journal |
| issn | 1682-0363 1819-3684 |
| language | English |
| last_indexed | 2024-04-10T01:12:32Z |
| publishDate | 2008-09-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj.art-eff1090167c74dedb955c9e5c75b7ed22023-03-13T09:58:08ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842008-09-01735810.20538/1682-0363-2008-3-5-82074Effect of Granotsit preparation on mesenchymal bone marrow stem cells in experimental model of secondary immune deficiency with the aid of cyclophosphanA. N. Baikov0V. P. Shakhov1N. Yu. Shelgaev2V. A. Serebryakova3Сибирский государственный медицинский университетМедицинская промышленная компания «Электропульс»Челябинский областной кожно-венерологический диспансерСибирский государственный медицинский университетExperiments with 60 male and female mice of Balb/c line with mass of 18—21 g have been carried out. All manipulations, including ether euthanasia, were performed within the framework of approved rules for experiments with experimental animals. The mice were divided into four groups each of 15 animals. The groups were injected with physiological salt solution (first group), cycliphosphan (second group), Granotsit preparation (recombinant granulocytic colony-stimulating factor) (third group), and cycliphosphan in Granotsit preparation (fourth group). We have studied the total number of leucocytes in blood, bone marrow cellularity, content of granulocytic- macrophage colony-forming cells and mesenchyma l precursors with the aid of tissue culture in vitro. It has been found that the Granotsit preparation can stimulate myelopoiesis (direct specific action) and mesenchymopo iesis (indirect action) in norm. When a cytostatic agent, the studied cytokine has a protective action on both myeloid and mesenchyma l precursors of bone marrow.https://bulletin.ssmu.ru/jour/article/view/3425мезенхимальные стволовые клеткимезенхимопоэзиммунодефицитыгранулоцитарный колониестимулирующий фактор |
| spellingShingle | A. N. Baikov V. P. Shakhov N. Yu. Shelgaev V. A. Serebryakova Effect of Granotsit preparation on mesenchymal bone marrow stem cells in experimental model of secondary immune deficiency with the aid of cyclophosphan Бюллетень сибирской медицины мезенхимальные стволовые клетки мезенхимопоэз иммунодефициты гранулоцитарный колониестимулирующий фактор |
| title | Effect of Granotsit preparation on mesenchymal bone marrow stem cells in experimental model of secondary immune deficiency with the aid of cyclophosphan |
| title_full | Effect of Granotsit preparation on mesenchymal bone marrow stem cells in experimental model of secondary immune deficiency with the aid of cyclophosphan |
| title_fullStr | Effect of Granotsit preparation on mesenchymal bone marrow stem cells in experimental model of secondary immune deficiency with the aid of cyclophosphan |
| title_full_unstemmed | Effect of Granotsit preparation on mesenchymal bone marrow stem cells in experimental model of secondary immune deficiency with the aid of cyclophosphan |
| title_short | Effect of Granotsit preparation on mesenchymal bone marrow stem cells in experimental model of secondary immune deficiency with the aid of cyclophosphan |
| title_sort | effect of granotsit preparation on mesenchymal bone marrow stem cells in experimental model of secondary immune deficiency with the aid of cyclophosphan |
| topic | мезенхимальные стволовые клетки мезенхимопоэз иммунодефициты гранулоцитарный колониестимулирующий фактор |
| url | https://bulletin.ssmu.ru/jour/article/view/3425 |
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