<i>Plasmodium vivax</i> MSP1-42 kD Variant Proteins Detected Naturally Induced IgG Antibodies in Patients Regardless of the Infecting Parasite Phenotype in Mesoamerica
Background: The serological tests using blood stage antigens might be helpful for detecting recent exposure to <i>Plasmodium</i> parasites, and seroepidemiological studies would aid in the elimination of malaria. This work produced recombinant proteins of PvMSP1<sub>42</sub>...
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MDPI AG
2023-03-01
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| Series: | Life |
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| Online Access: | https://www.mdpi.com/2075-1729/13/3/704 |
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| author | Lilia Gonzalez-Ceron Barbara Dema Olga L. Palomeque-Culebro Frida Santillan-Valenzuela Alberto Montoya Arturo Reyes-Sandoval |
| author_facet | Lilia Gonzalez-Ceron Barbara Dema Olga L. Palomeque-Culebro Frida Santillan-Valenzuela Alberto Montoya Arturo Reyes-Sandoval |
| author_sort | Lilia Gonzalez-Ceron |
| collection | DOAJ |
| description | Background: The serological tests using blood stage antigens might be helpful for detecting recent exposure to <i>Plasmodium</i> parasites, and seroepidemiological studies would aid in the elimination of malaria. This work produced recombinant proteins of PvMSP1<sub>42</sub> variants and evaluated their capacity to detect IgG antibodies in symptomatic patients from Mesoamerica. Methods: Three variant <i>Pvmsp1<sub>42</sub></i> genes were cloned in the pHL-sec plasmid, expressed in the Expi293F™ eukaryotic system, and the recombinant proteins were purified by affinity chromatography. Using an ELISA, 174 plasma or eluted samples from patients infected with different <i>P. vivax</i> haplotypes were evaluated against PvMSP1<sub>42</sub> proteins and to a native blood stage antigen (NBSA). Results: The antibody IgG OD values toward PvMSP142 variants (v88, v21, and v274) were heterogeneous (<i>n</i> = 178; median = 0.84 IQR 0.28–1.64). The correlation of IgG levels among all proteins was very high (spearman’s rho = 0.96–0.98; <i>p</i> < 0.0001), but was lower between them and the NBSA (rho = 0.771; <i>p</i> < 0.0001). In only a few samples, higher reactivity to the homologous protein was evident. Patients with a past infection who were seropositive had higher IgG levels and lower parasitemia levels than those who did not (<i>p</i> < 0.0001). Conclusions: The PvMSP1<sub>42</sub> variants were similarly efficient in detecting specific IgG antibodies in <i>P. vivax</i> patients from Mesoamerica, regardless of the infecting parasite’s haplotype, and might be good candidates for malaria surveillance and epidemiological studies in the region. |
| first_indexed | 2024-03-11T06:17:46Z |
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| institution | Directory Open Access Journal |
| issn | 2075-1729 |
| language | English |
| last_indexed | 2024-03-11T06:17:46Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Life |
| spelling | doaj.art-eff206dd3aec4f5e945fba3dc9b61ef12023-11-17T12:11:13ZengMDPI AGLife2075-17292023-03-0113370410.3390/life13030704<i>Plasmodium vivax</i> MSP1-42 kD Variant Proteins Detected Naturally Induced IgG Antibodies in Patients Regardless of the Infecting Parasite Phenotype in MesoamericaLilia Gonzalez-Ceron0Barbara Dema1Olga L. Palomeque-Culebro2Frida Santillan-Valenzuela3Alberto Montoya4Arturo Reyes-Sandoval5Regional Centre of Public Health Research, National Institute for Public Health Research, Tapachula 30700, MexicoPandemic Science Institute, Nuffield Department of Medicine, University of Oxford, Oxford OX3 7BN, UKRegional Centre of Public Health Research, National Institute for Public Health Research, Tapachula 30700, MexicoRegional Centre of Public Health Research, National Institute for Public Health Research, Tapachula 30700, MexicoParasitology Department, National Centre for Diagnosis Reference, Ministry of Health, Managua 11165, NicaraguaInstituto Politécnico Nacional (IPN), Unidad Adolfo López Mateos, Av. Luis Enrique Erro s/n., Mexico City 07738, MexicoBackground: The serological tests using blood stage antigens might be helpful for detecting recent exposure to <i>Plasmodium</i> parasites, and seroepidemiological studies would aid in the elimination of malaria. This work produced recombinant proteins of PvMSP1<sub>42</sub> variants and evaluated their capacity to detect IgG antibodies in symptomatic patients from Mesoamerica. Methods: Three variant <i>Pvmsp1<sub>42</sub></i> genes were cloned in the pHL-sec plasmid, expressed in the Expi293F™ eukaryotic system, and the recombinant proteins were purified by affinity chromatography. Using an ELISA, 174 plasma or eluted samples from patients infected with different <i>P. vivax</i> haplotypes were evaluated against PvMSP1<sub>42</sub> proteins and to a native blood stage antigen (NBSA). Results: The antibody IgG OD values toward PvMSP142 variants (v88, v21, and v274) were heterogeneous (<i>n</i> = 178; median = 0.84 IQR 0.28–1.64). The correlation of IgG levels among all proteins was very high (spearman’s rho = 0.96–0.98; <i>p</i> < 0.0001), but was lower between them and the NBSA (rho = 0.771; <i>p</i> < 0.0001). In only a few samples, higher reactivity to the homologous protein was evident. Patients with a past infection who were seropositive had higher IgG levels and lower parasitemia levels than those who did not (<i>p</i> < 0.0001). Conclusions: The PvMSP1<sub>42</sub> variants were similarly efficient in detecting specific IgG antibodies in <i>P. vivax</i> patients from Mesoamerica, regardless of the infecting parasite’s haplotype, and might be good candidates for malaria surveillance and epidemiological studies in the region.https://www.mdpi.com/2075-1729/13/3/704<i>Plasmodium vivax</i>merozoite surface antigen-1PvMSP1<sub>42</sub>recombinant proteinsnatural antibody responsesIgG |
| spellingShingle | Lilia Gonzalez-Ceron Barbara Dema Olga L. Palomeque-Culebro Frida Santillan-Valenzuela Alberto Montoya Arturo Reyes-Sandoval <i>Plasmodium vivax</i> MSP1-42 kD Variant Proteins Detected Naturally Induced IgG Antibodies in Patients Regardless of the Infecting Parasite Phenotype in Mesoamerica Life <i>Plasmodium vivax</i> merozoite surface antigen-1 PvMSP1<sub>42</sub> recombinant proteins natural antibody responses IgG |
| title | <i>Plasmodium vivax</i> MSP1-42 kD Variant Proteins Detected Naturally Induced IgG Antibodies in Patients Regardless of the Infecting Parasite Phenotype in Mesoamerica |
| title_full | <i>Plasmodium vivax</i> MSP1-42 kD Variant Proteins Detected Naturally Induced IgG Antibodies in Patients Regardless of the Infecting Parasite Phenotype in Mesoamerica |
| title_fullStr | <i>Plasmodium vivax</i> MSP1-42 kD Variant Proteins Detected Naturally Induced IgG Antibodies in Patients Regardless of the Infecting Parasite Phenotype in Mesoamerica |
| title_full_unstemmed | <i>Plasmodium vivax</i> MSP1-42 kD Variant Proteins Detected Naturally Induced IgG Antibodies in Patients Regardless of the Infecting Parasite Phenotype in Mesoamerica |
| title_short | <i>Plasmodium vivax</i> MSP1-42 kD Variant Proteins Detected Naturally Induced IgG Antibodies in Patients Regardless of the Infecting Parasite Phenotype in Mesoamerica |
| title_sort | i plasmodium vivax i msp1 42 kd variant proteins detected naturally induced igg antibodies in patients regardless of the infecting parasite phenotype in mesoamerica |
| topic | <i>Plasmodium vivax</i> merozoite surface antigen-1 PvMSP1<sub>42</sub> recombinant proteins natural antibody responses IgG |
| url | https://www.mdpi.com/2075-1729/13/3/704 |
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