Antidiarrhoeal screening of Himalayan edible plant Begonia rubrovenia and its marker followed by its validation using computational analysis
Abstract Background Diarrhoea has become one of the major areas of concern due to its high mortality rate contributing it to be the second largest cause of death in world. To explore the effectiveness of medicinal plant, the present investigation was undertaken to scientifically justify the traditio...
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| Format: | Article |
| Language: | English |
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SpringerOpen
2024-02-01
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| Series: | Future Journal of Pharmaceutical Sciences |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s43094-024-00592-7 |
| _version_ | 1797275585879212032 |
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| author | Rupali S. Prasad Jun M. Kalita Nitish Rai Nikhil Y. Yenorkar Suhas R. Dhaswadikar Pravesh Sharma Mahaveer Dhobi Damiki Laloo Saurabh K. Sinha Prakash R. Itankar Satyendra K. Prasad |
| author_facet | Rupali S. Prasad Jun M. Kalita Nitish Rai Nikhil Y. Yenorkar Suhas R. Dhaswadikar Pravesh Sharma Mahaveer Dhobi Damiki Laloo Saurabh K. Sinha Prakash R. Itankar Satyendra K. Prasad |
| author_sort | Rupali S. Prasad |
| collection | DOAJ |
| description | Abstract Background Diarrhoea has become one of the major areas of concern due to its high mortality rate contributing it to be the second largest cause of death in world. To explore the effectiveness of medicinal plant, the present investigation was undertaken to scientifically justify the traditional claim of the ethanolic root extract of the plant Begonia rubrovenia (EBV) against diarrhoea. Results EBV was standardized using HPLC with quercetin as marker and was further subjected to normal fecal excretion study at 100, 200 and 300 mg/kg, p.o. along with quercetin and loperamide. The study confirmed the effectiveness of EBV at 200 and 300 mg/kg followed by quercetin. In castor oil induced diarrhoea rat model, EBV at 200 and 300 mg/kg significantly delayed onset of diarrhoea, reduced the diarrhoeal faecal output which contributed in higher % protection. The effectiveness of EBV at 200 mg/kg was also confirmed through gastrointestinal motility, fluid accumulation and PGE2 induced enteropooling tests. EBV and its marker quercetin also reduced the elevated level of NO and cytokines and restored the alterations in antioxidant enzymes, ions and enhanced Na+/K+–ATPase activity. Molecular docking, dynamics and network pharmacology study confirmed the role of quercetin in modulating the inflammatory mediators IL-1β, TNF-α and EP3 prostanoid receptor, where quercetin formed more stable complex with EP3 prostanoid receptor. Conclusion The study has scientifically justified the traditional use of the plants B. rubrovenia in treating diarrhoea, where quercetin played a critical role in the observed antidiarrhoeal potential of B. rubrovenia contributing in maintaining electrolyte balance, antioxidant status and inhibiting inflammatory mediators. |
| first_indexed | 2024-03-07T15:16:35Z |
| format | Article |
| id | doaj.art-eff2456a8da741e092e36f5e30e9c3fd |
| institution | Directory Open Access Journal |
| issn | 2314-7253 |
| language | English |
| last_indexed | 2024-03-07T15:16:35Z |
| publishDate | 2024-02-01 |
| publisher | SpringerOpen |
| record_format | Article |
| series | Future Journal of Pharmaceutical Sciences |
| spelling | doaj.art-eff2456a8da741e092e36f5e30e9c3fd2024-03-05T17:55:13ZengSpringerOpenFuture Journal of Pharmaceutical Sciences2314-72532024-02-0110111510.1186/s43094-024-00592-7Antidiarrhoeal screening of Himalayan edible plant Begonia rubrovenia and its marker followed by its validation using computational analysisRupali S. Prasad0Jun M. Kalita1Nitish Rai2Nikhil Y. Yenorkar3Suhas R. Dhaswadikar4Pravesh Sharma5Mahaveer Dhobi6Damiki Laloo7Saurabh K. Sinha8Prakash R. Itankar9Satyendra K. Prasad10Department of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur UniversitySchool of Pharmaceutical Science, Girijananda Chowdhury UniversityDepartment of Biotechnology, Mohanlal Sukhadia UniversityDepartment of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur UniversityDepartment of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur UniversityBirla Institute of Technology and Sciences, PilaniDepartment of Pharmacognosy and Phytochemistry, Delhi Pharmaceutical Sciences and Research UniversitySchool of Pharmaceutical Science, Girijananda Chowdhury UniversityDepartment of Pharmaceutical Sciences, Mohanlal Sukhadia UniversityDepartment of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur UniversityDepartment of Pharmaceutical Sciences, Rashtrasant Tukadoji Maharaj Nagpur UniversityAbstract Background Diarrhoea has become one of the major areas of concern due to its high mortality rate contributing it to be the second largest cause of death in world. To explore the effectiveness of medicinal plant, the present investigation was undertaken to scientifically justify the traditional claim of the ethanolic root extract of the plant Begonia rubrovenia (EBV) against diarrhoea. Results EBV was standardized using HPLC with quercetin as marker and was further subjected to normal fecal excretion study at 100, 200 and 300 mg/kg, p.o. along with quercetin and loperamide. The study confirmed the effectiveness of EBV at 200 and 300 mg/kg followed by quercetin. In castor oil induced diarrhoea rat model, EBV at 200 and 300 mg/kg significantly delayed onset of diarrhoea, reduced the diarrhoeal faecal output which contributed in higher % protection. The effectiveness of EBV at 200 mg/kg was also confirmed through gastrointestinal motility, fluid accumulation and PGE2 induced enteropooling tests. EBV and its marker quercetin also reduced the elevated level of NO and cytokines and restored the alterations in antioxidant enzymes, ions and enhanced Na+/K+–ATPase activity. Molecular docking, dynamics and network pharmacology study confirmed the role of quercetin in modulating the inflammatory mediators IL-1β, TNF-α and EP3 prostanoid receptor, where quercetin formed more stable complex with EP3 prostanoid receptor. Conclusion The study has scientifically justified the traditional use of the plants B. rubrovenia in treating diarrhoea, where quercetin played a critical role in the observed antidiarrhoeal potential of B. rubrovenia contributing in maintaining electrolyte balance, antioxidant status and inhibiting inflammatory mediators.https://doi.org/10.1186/s43094-024-00592-7Begonia rubroveniaCytokinesDiarrhoea scoreEnteropooling testEP3 prostanoid receptorQuercetin |
| spellingShingle | Rupali S. Prasad Jun M. Kalita Nitish Rai Nikhil Y. Yenorkar Suhas R. Dhaswadikar Pravesh Sharma Mahaveer Dhobi Damiki Laloo Saurabh K. Sinha Prakash R. Itankar Satyendra K. Prasad Antidiarrhoeal screening of Himalayan edible plant Begonia rubrovenia and its marker followed by its validation using computational analysis Future Journal of Pharmaceutical Sciences Begonia rubrovenia Cytokines Diarrhoea score Enteropooling test EP3 prostanoid receptor Quercetin |
| title | Antidiarrhoeal screening of Himalayan edible plant Begonia rubrovenia and its marker followed by its validation using computational analysis |
| title_full | Antidiarrhoeal screening of Himalayan edible plant Begonia rubrovenia and its marker followed by its validation using computational analysis |
| title_fullStr | Antidiarrhoeal screening of Himalayan edible plant Begonia rubrovenia and its marker followed by its validation using computational analysis |
| title_full_unstemmed | Antidiarrhoeal screening of Himalayan edible plant Begonia rubrovenia and its marker followed by its validation using computational analysis |
| title_short | Antidiarrhoeal screening of Himalayan edible plant Begonia rubrovenia and its marker followed by its validation using computational analysis |
| title_sort | antidiarrhoeal screening of himalayan edible plant begonia rubrovenia and its marker followed by its validation using computational analysis |
| topic | Begonia rubrovenia Cytokines Diarrhoea score Enteropooling test EP3 prostanoid receptor Quercetin |
| url | https://doi.org/10.1186/s43094-024-00592-7 |
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