Exploring wild Aspleniaceae ferns as safety sources of polyphenols: The case of Asplenium trichomanes L. and Ceterach officinarum Willd.
The forest ecosystem is a source of material resources used since ancient times by mankind. Ferns are part of different oriental systems of traditional medicine due to the phytochemical variety of their fronds, which have allowed their traditional use to be validated through ethnopharmacological stu...
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| Language: | English |
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Frontiers Media S.A.
2022-09-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.994215/full |
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| author | Adrià Farràs Adrià Farràs Montserrat Mitjans Filippo Maggi Giovanni Caprioli María Pilar Vinardell Víctor López Víctor López |
| author_facet | Adrià Farràs Adrià Farràs Montserrat Mitjans Filippo Maggi Giovanni Caprioli María Pilar Vinardell Víctor López Víctor López |
| author_sort | Adrià Farràs |
| collection | DOAJ |
| description | The forest ecosystem is a source of material resources used since ancient times by mankind. Ferns are part of different oriental systems of traditional medicine due to the phytochemical variety of their fronds, which have allowed their traditional use to be validated through ethnopharmacological studies. In Europe, different cultures have used the same fern with a wide variety of applications due to its presence in most European forests. In recent years, studies on the phytocharacterization and biological activity of the fronds of the main European ferns have been published. In this study, the presence of polyphenolic phytochemicals has been evaluated by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) in the fronds of two wild ferns together with in vitro activities in non-tumoral and human tumoral cell lines. The polyphenols were extracted from Asplenium trichomanes L. and Ceterach officinarum Willd. by cold maceration using methanol. The main phytochemicals of polyphenolic origin in the extracts of A. trichomanes and C. officinarum determined by HPLC-MS/MS were the flavonol hyperoside and the phenolic acid chlorogenic acid, respectively. This different polyphenolic nature of both extracts contributes to the divergence of the behavior experienced in the biological activities tested, but none of the extracts showed a cytotoxic or phototoxic profile in the different tested cell lines. However, the cytoprotective values in front of the H2O2 oxidative stress induced in the 3T3 and HaCaT cell lines position these extracts as possible candidates for future health applications. |
| first_indexed | 2024-04-12T22:58:28Z |
| format | Article |
| id | doaj.art-eff6000d678849a0b1f9925a17de70ea |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-12T22:58:28Z |
| publishDate | 2022-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-eff6000d678849a0b1f9925a17de70ea2022-12-22T03:13:08ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-09-01910.3389/fnut.2022.994215994215Exploring wild Aspleniaceae ferns as safety sources of polyphenols: The case of Asplenium trichomanes L. and Ceterach officinarum Willd.Adrià Farràs0Adrià Farràs1Montserrat Mitjans2Filippo Maggi3Giovanni Caprioli4María Pilar Vinardell5Víctor López6Víctor López7Department of Biochemistry and Physiology, Faculty of Pharmacy and Food Sciences, Universitat de Barcelona, Barcelona, SpainDepartment of Pharmacy, Faculty of Health Sciences, Universidad San Jorge, Zaragoza, SpainDepartment of Biochemistry and Physiology, Faculty of Pharmacy and Food Sciences, Universitat de Barcelona, Barcelona, SpainSchool of Pharmacy, Università di Camerino, Camerino, ItalySchool of Pharmacy, Università di Camerino, Camerino, ItalyDepartment of Biochemistry and Physiology, Faculty of Pharmacy and Food Sciences, Universitat de Barcelona, Barcelona, SpainDepartment of Pharmacy, Faculty of Health Sciences, Universidad San Jorge, Zaragoza, SpainInstituto Agroalimentario de Aragón-IA2, CITA-Universidad de Zaragoza, Zaragoza, SpainThe forest ecosystem is a source of material resources used since ancient times by mankind. Ferns are part of different oriental systems of traditional medicine due to the phytochemical variety of their fronds, which have allowed their traditional use to be validated through ethnopharmacological studies. In Europe, different cultures have used the same fern with a wide variety of applications due to its presence in most European forests. In recent years, studies on the phytocharacterization and biological activity of the fronds of the main European ferns have been published. In this study, the presence of polyphenolic phytochemicals has been evaluated by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) in the fronds of two wild ferns together with in vitro activities in non-tumoral and human tumoral cell lines. The polyphenols were extracted from Asplenium trichomanes L. and Ceterach officinarum Willd. by cold maceration using methanol. The main phytochemicals of polyphenolic origin in the extracts of A. trichomanes and C. officinarum determined by HPLC-MS/MS were the flavonol hyperoside and the phenolic acid chlorogenic acid, respectively. This different polyphenolic nature of both extracts contributes to the divergence of the behavior experienced in the biological activities tested, but none of the extracts showed a cytotoxic or phototoxic profile in the different tested cell lines. However, the cytoprotective values in front of the H2O2 oxidative stress induced in the 3T3 and HaCaT cell lines position these extracts as possible candidates for future health applications.https://www.frontiersin.org/articles/10.3389/fnut.2022.994215/fullbioeconomycytoprotectioncytotoxictyethnopharmacologyfernsfunctional food |
| spellingShingle | Adrià Farràs Adrià Farràs Montserrat Mitjans Filippo Maggi Giovanni Caprioli María Pilar Vinardell Víctor López Víctor López Exploring wild Aspleniaceae ferns as safety sources of polyphenols: The case of Asplenium trichomanes L. and Ceterach officinarum Willd. Frontiers in Nutrition bioeconomy cytoprotection cytotoxicty ethnopharmacology ferns functional food |
| title | Exploring wild Aspleniaceae ferns as safety sources of polyphenols: The case of Asplenium trichomanes L. and Ceterach officinarum Willd. |
| title_full | Exploring wild Aspleniaceae ferns as safety sources of polyphenols: The case of Asplenium trichomanes L. and Ceterach officinarum Willd. |
| title_fullStr | Exploring wild Aspleniaceae ferns as safety sources of polyphenols: The case of Asplenium trichomanes L. and Ceterach officinarum Willd. |
| title_full_unstemmed | Exploring wild Aspleniaceae ferns as safety sources of polyphenols: The case of Asplenium trichomanes L. and Ceterach officinarum Willd. |
| title_short | Exploring wild Aspleniaceae ferns as safety sources of polyphenols: The case of Asplenium trichomanes L. and Ceterach officinarum Willd. |
| title_sort | exploring wild aspleniaceae ferns as safety sources of polyphenols the case of asplenium trichomanes l and ceterach officinarum willd |
| topic | bioeconomy cytoprotection cytotoxicty ethnopharmacology ferns functional food |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.994215/full |
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