Natural Materials Modified and Applied to the Detection of Drugs In Situ: Modification of Eggshell and Quantification of Oxytetracycline
This work describes a novel sensing system using eggshells as substrate for the first time, targeting the detection and semiquantitative determination of antibiotics in waters from aquaculture, enabling simple, inexpensive, and in situ drug monitoring. Eggshell was ground and the resulting powder wa...
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| Format: | Article |
| Language: | English |
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MDPI AG
2022-08-01
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| Series: | Sensors |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1424-8220/22/15/5746 |
| _version_ | 1797432670045601792 |
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| author | Helena I. A. S. Gomes M. Goreti F. Sales |
| author_facet | Helena I. A. S. Gomes M. Goreti F. Sales |
| author_sort | Helena I. A. S. Gomes |
| collection | DOAJ |
| description | This work describes a novel sensing system using eggshells as substrate for the first time, targeting the detection and semiquantitative determination of antibiotics in waters from aquaculture, enabling simple, inexpensive, and in situ drug monitoring. Eggshell was ground and the resulting powder was modified by adsorption of suitable reagents, and it takes a typical colour after contact with the antibiotic. The colour intensity is correlated with the concentration of the antibiotic. This novel approach was applied to oxytetracycline, one of the antibiotics commonly used in aquaculture. The chemical changes on the eggshell powder were evaluated and optimised to produce an intense colour change as a function of the concentration of the antibiotic. The colour changes were evaluated by visual comparison with images taken with a digital camera, applying an appropriate mathematical treatment to the colour coordinates of the HSL system used by Windows. The selectivity of the response was tested against other antibiotic drugs. The materials were also used in the analysis of a spiked environmental water sample. Overall, this work presents a rapid, inexpensive, simple and equipment-free method for screening and discrimination of tetracycline drugs in aquaculture. The method is a green approach by reusing eggshells and decreasing the level of contamination correlated to analytical methods, thus being a promising tool for local, rapid, and cost-effective antibiotic monitoring. |
| first_indexed | 2024-03-09T10:05:58Z |
| format | Article |
| id | doaj.art-eff6874ad1d449ba925a019f284d1949 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-09T10:05:58Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-eff6874ad1d449ba925a019f284d19492023-12-01T23:10:11ZengMDPI AGSensors1424-82202022-08-012215574610.3390/s22155746Natural Materials Modified and Applied to the Detection of Drugs In Situ: Modification of Eggshell and Quantification of OxytetracyclineHelena I. A. S. Gomes0M. Goreti F. Sales1BioMark@ISEP/CEB—Centre of Biological Engineering/LABBELS, School of Engineering, Polytechnic Institute of Porto, Rua Dr. António Bernardino de Almeida, 4249-015 Porto, PortugalBioMark@ISEP/CEB—Centre of Biological Engineering/LABBELS, School of Engineering, Polytechnic Institute of Porto, Rua Dr. António Bernardino de Almeida, 4249-015 Porto, PortugalThis work describes a novel sensing system using eggshells as substrate for the first time, targeting the detection and semiquantitative determination of antibiotics in waters from aquaculture, enabling simple, inexpensive, and in situ drug monitoring. Eggshell was ground and the resulting powder was modified by adsorption of suitable reagents, and it takes a typical colour after contact with the antibiotic. The colour intensity is correlated with the concentration of the antibiotic. This novel approach was applied to oxytetracycline, one of the antibiotics commonly used in aquaculture. The chemical changes on the eggshell powder were evaluated and optimised to produce an intense colour change as a function of the concentration of the antibiotic. The colour changes were evaluated by visual comparison with images taken with a digital camera, applying an appropriate mathematical treatment to the colour coordinates of the HSL system used by Windows. The selectivity of the response was tested against other antibiotic drugs. The materials were also used in the analysis of a spiked environmental water sample. Overall, this work presents a rapid, inexpensive, simple and equipment-free method for screening and discrimination of tetracycline drugs in aquaculture. The method is a green approach by reusing eggshells and decreasing the level of contamination correlated to analytical methods, thus being a promising tool for local, rapid, and cost-effective antibiotic monitoring.https://www.mdpi.com/1424-8220/22/15/5746eggshell substrateantibioticwaterenvironmentaquaculture |
| spellingShingle | Helena I. A. S. Gomes M. Goreti F. Sales Natural Materials Modified and Applied to the Detection of Drugs In Situ: Modification of Eggshell and Quantification of Oxytetracycline Sensors eggshell substrate antibiotic water environment aquaculture |
| title | Natural Materials Modified and Applied to the Detection of Drugs In Situ: Modification of Eggshell and Quantification of Oxytetracycline |
| title_full | Natural Materials Modified and Applied to the Detection of Drugs In Situ: Modification of Eggshell and Quantification of Oxytetracycline |
| title_fullStr | Natural Materials Modified and Applied to the Detection of Drugs In Situ: Modification of Eggshell and Quantification of Oxytetracycline |
| title_full_unstemmed | Natural Materials Modified and Applied to the Detection of Drugs In Situ: Modification of Eggshell and Quantification of Oxytetracycline |
| title_short | Natural Materials Modified and Applied to the Detection of Drugs In Situ: Modification of Eggshell and Quantification of Oxytetracycline |
| title_sort | natural materials modified and applied to the detection of drugs in situ modification of eggshell and quantification of oxytetracycline |
| topic | eggshell substrate antibiotic water environment aquaculture |
| url | https://www.mdpi.com/1424-8220/22/15/5746 |
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