Quantification of Mouse Embryonic Eye Development with Optical Coherence Tomography In Utero
Mouse models are commonly used as research tools to understand regulatory pathways affected by human diseases and disorders. Live imaging tools for visualization of mouse embryonic ocular tissues would be beneficial in research associated with developmental ocular defects. In this study, in utero qu...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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Samara National Research University
2015-03-01
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| Series: | Journal of Biomedical Photonics & Engineering |
| Subjects: | |
| Online Access: | http://jbpe.ssau.ru/index.php/JBPE/article/view/2039 |
| _version_ | 1818061719396679680 |
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| author | Narendran Sudheendran Maleeha Mashiatulla Raksha Raghunathan Saba H. Syed Mary E. Dickinson Irina V. Larina Kirill V. Larin |
| author_facet | Narendran Sudheendran Maleeha Mashiatulla Raksha Raghunathan Saba H. Syed Mary E. Dickinson Irina V. Larina Kirill V. Larin |
| author_sort | Narendran Sudheendran |
| collection | DOAJ |
| description | Mouse models are commonly used as research tools to understand regulatory pathways affected by human diseases and disorders. Live imaging tools for visualization of mouse embryonic ocular tissues would be beneficial in research associated with developmental ocular defects. In this study, in utero quantitative assessment of ocular structures in mouse embryos was performed with a swept-source optical coherence tomography (SSOCT). To define developmental changes in eye morphology in live embryos, the volume of the embryonic eye lens and the globe at different embryonic stages ranging from E13.5 to E18.5 was quantified. It is determined that the major axis diameter of the eye lens and the globe was found to increase from 0.44±0.18 mm to 0.98±0.05 mm and from 0.56±0.22 mm to 1.23±0.14 mm, respectively, as the embryo ages from E13.5 to E18.5. For the same stages, the volume of the eye lens and globe was found to increase from 0.028±0.027 mm3 to 0.32±0.08 mm3 and from 0.085±0.08 mm3 to 0.75±0.27 mm3, respectively. These results suggest that OCT can accurately assess developmental processes of ocular structures and can be potentially used to assess embryonic ocular growth in mouse mutants with eye abnormalities and to study the effect of toxicological and pharmacological agents. |
| first_indexed | 2024-12-10T13:52:47Z |
| format | Article |
| id | doaj.art-eff7a6f8ae034530a9405aca1989c798 |
| institution | Directory Open Access Journal |
| issn | 2411-2844 |
| language | English |
| last_indexed | 2024-12-10T13:52:47Z |
| publishDate | 2015-03-01 |
| publisher | Samara National Research University |
| record_format | Article |
| series | Journal of Biomedical Photonics & Engineering |
| spelling | doaj.art-eff7a6f8ae034530a9405aca1989c7982022-12-22T01:46:08ZengSamara National Research UniversityJournal of Biomedical Photonics & Engineering2411-28442015-03-0111909510.18287/jbpe-2015-1-1-902344Quantification of Mouse Embryonic Eye Development with Optical Coherence Tomography In UteroNarendran Sudheendran0Maleeha Mashiatulla1Raksha Raghunathan2Saba H. Syed3Mary E. Dickinson4Irina V. Larina5Kirill V. Larin6Department of Biomedical Engineering, University of Houston, TX, USADepartment of Biomedical Engineering, University of Houston, TX, USADepartment of Biomedical Engineering, University of Houston, TX, USADepartment Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX, USADepartment Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX, USADepartment Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX, USADepartment of Biomedical Engineering, University of Houston, TX, USA; Department Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, TX, USA; Institute of Optics and Biophotonics, Saratov State University, RussiaMouse models are commonly used as research tools to understand regulatory pathways affected by human diseases and disorders. Live imaging tools for visualization of mouse embryonic ocular tissues would be beneficial in research associated with developmental ocular defects. In this study, in utero quantitative assessment of ocular structures in mouse embryos was performed with a swept-source optical coherence tomography (SSOCT). To define developmental changes in eye morphology in live embryos, the volume of the embryonic eye lens and the globe at different embryonic stages ranging from E13.5 to E18.5 was quantified. It is determined that the major axis diameter of the eye lens and the globe was found to increase from 0.44±0.18 mm to 0.98±0.05 mm and from 0.56±0.22 mm to 1.23±0.14 mm, respectively, as the embryo ages from E13.5 to E18.5. For the same stages, the volume of the eye lens and globe was found to increase from 0.028±0.027 mm3 to 0.32±0.08 mm3 and from 0.085±0.08 mm3 to 0.75±0.27 mm3, respectively. These results suggest that OCT can accurately assess developmental processes of ocular structures and can be potentially used to assess embryonic ocular growth in mouse mutants with eye abnormalities and to study the effect of toxicological and pharmacological agents.http://jbpe.ssau.ru/index.php/JBPE/article/view/2039Eye volumein uteromouse embryooptical coherence tomography |
| spellingShingle | Narendran Sudheendran Maleeha Mashiatulla Raksha Raghunathan Saba H. Syed Mary E. Dickinson Irina V. Larina Kirill V. Larin Quantification of Mouse Embryonic Eye Development with Optical Coherence Tomography In Utero Journal of Biomedical Photonics & Engineering Eye volume in utero mouse embryo optical coherence tomography |
| title | Quantification of Mouse Embryonic Eye Development with Optical Coherence Tomography In Utero |
| title_full | Quantification of Mouse Embryonic Eye Development with Optical Coherence Tomography In Utero |
| title_fullStr | Quantification of Mouse Embryonic Eye Development with Optical Coherence Tomography In Utero |
| title_full_unstemmed | Quantification of Mouse Embryonic Eye Development with Optical Coherence Tomography In Utero |
| title_short | Quantification of Mouse Embryonic Eye Development with Optical Coherence Tomography In Utero |
| title_sort | quantification of mouse embryonic eye development with optical coherence tomography in utero |
| topic | Eye volume in utero mouse embryo optical coherence tomography |
| url | http://jbpe.ssau.ru/index.php/JBPE/article/view/2039 |
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