Genetic characterization of Cryptosporidium spp. and Giardia duodenalis in dogs and cats in Guangdong, China
Abstract Background There are only limited number of reports on molecular epidemiology of Cryptosporidium spp. and Giardia duodenalis in dogs and cats in China. This study was conducted to assess the infection rates, genetic identity, and public health potential of these parasites in dogs and cats i...
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| Format: | Article |
| Language: | English |
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BMC
2019-11-01
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| Series: | Parasites & Vectors |
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| Online Access: | https://doi.org/10.1186/s13071-019-3822-z |
| _version_ | 1818647649076641792 |
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| author | Jiayu Li Xiaoyu Dan Kexin Zhu Na Li Yaqiong Guo Zezhong Zheng Yaoyu Feng Lihua Xiao |
| author_facet | Jiayu Li Xiaoyu Dan Kexin Zhu Na Li Yaqiong Guo Zezhong Zheng Yaoyu Feng Lihua Xiao |
| author_sort | Jiayu Li |
| collection | DOAJ |
| description | Abstract Background There are only limited number of reports on molecular epidemiology of Cryptosporidium spp. and Giardia duodenalis in dogs and cats in China. This study was conducted to assess the infection rates, genetic identity, and public health potential of these parasites in dogs and cats in Guangdong, China. Methods PCR and sequence analyses were used to identify and genotype Cryptosporidium spp. and G. duodenalis in fecal samples from 641 dogs and 418 cats in Guangdong. Chi-square test and odds ratio analysis were used to compare the occurrence rates of these pathogens and identify risk factors for infection. Results The overall infection rates of Cryptosporidium spp. and G. duodenalis were 6.9% (44/641) and 9.4% (60/641) in dogs, and 6.2% (26/418) and 3.6% (15/418) in cats. Purebred cats (12.4%; χ 2 = 5.110, OR = 2.8, P = 0.024) and dogs (10.8%; χ 2 = 5.597, OR = 4.8, P = 0.018) were more likely to be infected by Cryptosporidium spp. and G. duodenalis, respectively. Dogs (12.0%; χ 2 = 7.589, OR = 2.6, P = 0.006) and cats (13.6%; χ 2 = 8.235, OR = 3.5, P = 0.004) under 6 months had significantly higher infection rates of Cryptosporidium spp. than older animals. Household (13.9%; χ 2 = 10.279, OR = 2.6, P = 0.008) and pet shop dogs (11.0%; χ 2 = 7.182, OR = 2.0, P = 0.048) had higher occurrence of Cryptosporidium spp., as was the case for G. duodenalis occurrence in experimental dogs (13.4%; χ 2 = 9.223, OR = 1.9, P = 0.017). Cryptosporidium canis (n = 42), C. muris (n = 1) and Cryptosporidium rat genotype IV (n = 1) were identified in dogs, while C. felis (n = 21), C. parvum (n = 3), C. muris (n = 1) and Cryptosporidium rat genotype IV (n = 1) were identified in cats. In contrast, the canine-specific assemblages C (n = 27) and D (n = 26) and the feline-specific assemblage F (n = 14) were almost exclusively the only genotypes of G. duodenalis in dogs and cats, respectively. There was no significant difference in infection rates of Cryptosporidium spp. and G. duodenalis between diarrheal and non-diarrheal pets. Conclusions While domestic pets in Guangdong are infected with zoonotic Cryptosporidium species, they are mainly infected with host-specific G. duodenalis genotypes. Risk factors for infections differ between Cryptosporidium spp. and G. duodenalis and between dogs and cats. |
| first_indexed | 2024-12-17T01:05:53Z |
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| id | doaj.art-eff8d7ebd418428ea80412a01e2d988e |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-17T01:05:53Z |
| publishDate | 2019-11-01 |
| publisher | BMC |
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| series | Parasites & Vectors |
| spelling | doaj.art-eff8d7ebd418428ea80412a01e2d988e2022-12-21T22:09:16ZengBMCParasites & Vectors1756-33052019-11-011211910.1186/s13071-019-3822-zGenetic characterization of Cryptosporidium spp. and Giardia duodenalis in dogs and cats in Guangdong, ChinaJiayu Li0Xiaoyu Dan1Kexin Zhu2Na Li3Yaqiong Guo4Zezhong Zheng5Yaoyu Feng6Lihua Xiao7Key Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural UniversityKey Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural UniversityKey Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural UniversityKey Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural UniversityKey Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural UniversityKey Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural UniversityKey Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural UniversityKey Laboratory of Zoonosis of Ministry of Agriculture, College of Veterinary Medicine, South China Agricultural UniversityAbstract Background There are only limited number of reports on molecular epidemiology of Cryptosporidium spp. and Giardia duodenalis in dogs and cats in China. This study was conducted to assess the infection rates, genetic identity, and public health potential of these parasites in dogs and cats in Guangdong, China. Methods PCR and sequence analyses were used to identify and genotype Cryptosporidium spp. and G. duodenalis in fecal samples from 641 dogs and 418 cats in Guangdong. Chi-square test and odds ratio analysis were used to compare the occurrence rates of these pathogens and identify risk factors for infection. Results The overall infection rates of Cryptosporidium spp. and G. duodenalis were 6.9% (44/641) and 9.4% (60/641) in dogs, and 6.2% (26/418) and 3.6% (15/418) in cats. Purebred cats (12.4%; χ 2 = 5.110, OR = 2.8, P = 0.024) and dogs (10.8%; χ 2 = 5.597, OR = 4.8, P = 0.018) were more likely to be infected by Cryptosporidium spp. and G. duodenalis, respectively. Dogs (12.0%; χ 2 = 7.589, OR = 2.6, P = 0.006) and cats (13.6%; χ 2 = 8.235, OR = 3.5, P = 0.004) under 6 months had significantly higher infection rates of Cryptosporidium spp. than older animals. Household (13.9%; χ 2 = 10.279, OR = 2.6, P = 0.008) and pet shop dogs (11.0%; χ 2 = 7.182, OR = 2.0, P = 0.048) had higher occurrence of Cryptosporidium spp., as was the case for G. duodenalis occurrence in experimental dogs (13.4%; χ 2 = 9.223, OR = 1.9, P = 0.017). Cryptosporidium canis (n = 42), C. muris (n = 1) and Cryptosporidium rat genotype IV (n = 1) were identified in dogs, while C. felis (n = 21), C. parvum (n = 3), C. muris (n = 1) and Cryptosporidium rat genotype IV (n = 1) were identified in cats. In contrast, the canine-specific assemblages C (n = 27) and D (n = 26) and the feline-specific assemblage F (n = 14) were almost exclusively the only genotypes of G. duodenalis in dogs and cats, respectively. There was no significant difference in infection rates of Cryptosporidium spp. and G. duodenalis between diarrheal and non-diarrheal pets. Conclusions While domestic pets in Guangdong are infected with zoonotic Cryptosporidium species, they are mainly infected with host-specific G. duodenalis genotypes. Risk factors for infections differ between Cryptosporidium spp. and G. duodenalis and between dogs and cats.https://doi.org/10.1186/s13071-019-3822-zCryptosporidium spp.Giardia duodenalisGenotypeRisk factors |
| spellingShingle | Jiayu Li Xiaoyu Dan Kexin Zhu Na Li Yaqiong Guo Zezhong Zheng Yaoyu Feng Lihua Xiao Genetic characterization of Cryptosporidium spp. and Giardia duodenalis in dogs and cats in Guangdong, China Parasites & Vectors Cryptosporidium spp. Giardia duodenalis Genotype Risk factors |
| title | Genetic characterization of Cryptosporidium spp. and Giardia duodenalis in dogs and cats in Guangdong, China |
| title_full | Genetic characterization of Cryptosporidium spp. and Giardia duodenalis in dogs and cats in Guangdong, China |
| title_fullStr | Genetic characterization of Cryptosporidium spp. and Giardia duodenalis in dogs and cats in Guangdong, China |
| title_full_unstemmed | Genetic characterization of Cryptosporidium spp. and Giardia duodenalis in dogs and cats in Guangdong, China |
| title_short | Genetic characterization of Cryptosporidium spp. and Giardia duodenalis in dogs and cats in Guangdong, China |
| title_sort | genetic characterization of cryptosporidium spp and giardia duodenalis in dogs and cats in guangdong china |
| topic | Cryptosporidium spp. Giardia duodenalis Genotype Risk factors |
| url | https://doi.org/10.1186/s13071-019-3822-z |
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