Rhoifolin regulates oxidative stress and proinflammatory cytokine levels in Freund’s adjuvant-induced rheumatoid arthritis via inhibition of NF-κB
Rheumatoid arthritis (RA) is an autoimmune disease of knee joints involving pain and inflammation. Rhoifolin is a plant flavonoid known to have antioxidant and anti-inflammatory properties. This study was taken to identify the effect of rhoifolin on complete Freund’s adjuvant (CFA)-induced arthritis...
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| Format: | Article |
| Language: | English |
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Associação Brasileira de Divulgação Científica
2020-05-01
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| Series: | Brazilian Journal of Medical and Biological Research |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000600606&tlng=en |
| _version_ | 1798019859374669824 |
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| author | Shanqin Peng Congqi Hu Xi Liu Lei Lei Guodong He Chenming Xiong Wenqian Wu |
| author_facet | Shanqin Peng Congqi Hu Xi Liu Lei Lei Guodong He Chenming Xiong Wenqian Wu |
| author_sort | Shanqin Peng |
| collection | DOAJ |
| description | Rheumatoid arthritis (RA) is an autoimmune disease of knee joints involving pain and inflammation. Rhoifolin is a plant flavonoid known to have antioxidant and anti-inflammatory properties. This study was taken to identify the effect of rhoifolin on complete Freund’s adjuvant (CFA)-induced arthritis in the rat model. Treatment with rhoifolin (10 and 20 mg/kg) showed a significant improvement in the overall health parameters such as paw edema and weight loss. This improvement in morphological parameters corroborated the findings with gross morphological changes observed in the histopathological analysis. Rhoifolin treatment also caused a significant decrease in oxidative stress, evident from changes in intracellular levels of glutathione, glutathione peroxidase, malondialdehyde, and superoxide dismutase in the articular cartilage tissue. Moreover, proinflammatory cytokines, tumor necrosis factor (TNF)-α, interleukin(IL)-1β, and IL-6 showed a significant downregulation of gene expression and intracellular protein concentration levels. The NF-κB pathway showed a significant attenuation as evident in the significant reduction in the levels of NF-κB p65 and p-IκB-α. These results indicated that rhoifolin can be a natural therapeutic alternative to the extant regimens, which include non-steroidal anti-inflammatory drugs and immunosuppressants. Additionally, the antioxidant and anti-inflammatory action of rhoifolin was probably mediated by the NF-κB pathway. However, the exact target molecules of this pathway need to be determined in further studies. |
| first_indexed | 2024-04-11T16:47:49Z |
| format | Article |
| id | doaj.art-eff94ce60c9a4a4aa6cf7f8a90847fb2 |
| institution | Directory Open Access Journal |
| issn | 1414-431X |
| language | English |
| last_indexed | 2024-04-11T16:47:49Z |
| publishDate | 2020-05-01 |
| publisher | Associação Brasileira de Divulgação Científica |
| record_format | Article |
| series | Brazilian Journal of Medical and Biological Research |
| spelling | doaj.art-eff94ce60c9a4a4aa6cf7f8a90847fb22022-12-22T04:13:32ZengAssociação Brasileira de Divulgação CientíficaBrazilian Journal of Medical and Biological Research1414-431X2020-05-0153610.1590/1414-431x20209489Rhoifolin regulates oxidative stress and proinflammatory cytokine levels in Freund’s adjuvant-induced rheumatoid arthritis via inhibition of NF-κBShanqin Penghttps://orcid.org/0000-0002-0800-4247Congqi Huhttps://orcid.org/0000-0001-9711-6998Xi Liuhttps://orcid.org/0000-0003-1527-9836Lei Leihttps://orcid.org/0000-0003-1490-1419Guodong Hehttps://orcid.org/0000-0003-3249-9419Chenming Xionghttps://orcid.org/0000-0001-8580-8831Wenqian Wuhttps://orcid.org/0000-0002-9369-7817Rheumatoid arthritis (RA) is an autoimmune disease of knee joints involving pain and inflammation. Rhoifolin is a plant flavonoid known to have antioxidant and anti-inflammatory properties. This study was taken to identify the effect of rhoifolin on complete Freund’s adjuvant (CFA)-induced arthritis in the rat model. Treatment with rhoifolin (10 and 20 mg/kg) showed a significant improvement in the overall health parameters such as paw edema and weight loss. This improvement in morphological parameters corroborated the findings with gross morphological changes observed in the histopathological analysis. Rhoifolin treatment also caused a significant decrease in oxidative stress, evident from changes in intracellular levels of glutathione, glutathione peroxidase, malondialdehyde, and superoxide dismutase in the articular cartilage tissue. Moreover, proinflammatory cytokines, tumor necrosis factor (TNF)-α, interleukin(IL)-1β, and IL-6 showed a significant downregulation of gene expression and intracellular protein concentration levels. The NF-κB pathway showed a significant attenuation as evident in the significant reduction in the levels of NF-κB p65 and p-IκB-α. These results indicated that rhoifolin can be a natural therapeutic alternative to the extant regimens, which include non-steroidal anti-inflammatory drugs and immunosuppressants. Additionally, the antioxidant and anti-inflammatory action of rhoifolin was probably mediated by the NF-κB pathway. However, the exact target molecules of this pathway need to be determined in further studies.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000600606&tlng=enFlavonoidsRheumatoid arthritisOxidative stressAnti-inflammatoryCytokines |
| spellingShingle | Shanqin Peng Congqi Hu Xi Liu Lei Lei Guodong He Chenming Xiong Wenqian Wu Rhoifolin regulates oxidative stress and proinflammatory cytokine levels in Freund’s adjuvant-induced rheumatoid arthritis via inhibition of NF-κB Brazilian Journal of Medical and Biological Research Flavonoids Rheumatoid arthritis Oxidative stress Anti-inflammatory Cytokines |
| title | Rhoifolin regulates oxidative stress and proinflammatory cytokine levels in Freund’s adjuvant-induced rheumatoid arthritis via inhibition of NF-κB |
| title_full | Rhoifolin regulates oxidative stress and proinflammatory cytokine levels in Freund’s adjuvant-induced rheumatoid arthritis via inhibition of NF-κB |
| title_fullStr | Rhoifolin regulates oxidative stress and proinflammatory cytokine levels in Freund’s adjuvant-induced rheumatoid arthritis via inhibition of NF-κB |
| title_full_unstemmed | Rhoifolin regulates oxidative stress and proinflammatory cytokine levels in Freund’s adjuvant-induced rheumatoid arthritis via inhibition of NF-κB |
| title_short | Rhoifolin regulates oxidative stress and proinflammatory cytokine levels in Freund’s adjuvant-induced rheumatoid arthritis via inhibition of NF-κB |
| title_sort | rhoifolin regulates oxidative stress and proinflammatory cytokine levels in freund s adjuvant induced rheumatoid arthritis via inhibition of nf κb |
| topic | Flavonoids Rheumatoid arthritis Oxidative stress Anti-inflammatory Cytokines |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000600606&tlng=en |
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