Phloem Exudate Protein Profiles during Drought and Recovery Reveal Abiotic Stress Responses in Tomato Vasculature
Drought is the leading cause of agricultural yield loss among all abiotic stresses, and the link between water deficit and phloem protein contents is relatively unexplored. Here we collected phloem exudates from <i>Solanum lycopersicum</i> leaves during periods of drought stress and reco...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/12/4461 |
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| author | Aaron J. Ogden Jishnu J. Bhatt Heather M. Brewer Jack Kintigh Samwel M. Kariuki Sairam Rudrabhatla Joshua N. Adkins Wayne R. Curtis |
| author_facet | Aaron J. Ogden Jishnu J. Bhatt Heather M. Brewer Jack Kintigh Samwel M. Kariuki Sairam Rudrabhatla Joshua N. Adkins Wayne R. Curtis |
| author_sort | Aaron J. Ogden |
| collection | DOAJ |
| description | Drought is the leading cause of agricultural yield loss among all abiotic stresses, and the link between water deficit and phloem protein contents is relatively unexplored. Here we collected phloem exudates from <i>Solanum lycopersicum</i> leaves during periods of drought stress and recovery. Our analysis identified 2558 proteins, the most abundant of which were previously localized to the phloem. Independent of drought, enrichment analysis of the total phloem exudate protein profiles from all samples suggests that the protein content of phloem sap is complex, and includes proteins that function in chaperone systems, branched-chain amino acid synthesis, trehalose metabolism, and RNA silencing. We observed 169 proteins whose abundance changed significantly within the phloem sap, either during drought or recovery. Proteins that became significantly more abundant during drought include members of lipid metabolism, chaperone-mediated protein folding, carboxylic acid metabolism, abscisic acid signaling, cytokinin biosynthesis, and amino acid metabolism. Conversely, proteins involved in lipid signaling, sphingolipid metabolism, cell wall organization, carbohydrate metabolism, and a mitogen-activated protein kinase are decreased during drought. Our experiment has achieved an in-depth profiling of phloem sap protein contents during drought stress and recovery that supports previous findings and provides new evidence that multiple biological processes are involved in drought adaptation. |
| first_indexed | 2024-03-10T18:56:51Z |
| format | Article |
| id | doaj.art-eff9529471c547269707fdf45cef4902 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T18:56:51Z |
| publishDate | 2020-06-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-eff9529471c547269707fdf45cef49022023-11-20T04:45:24ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-06-012112446110.3390/ijms21124461Phloem Exudate Protein Profiles during Drought and Recovery Reveal Abiotic Stress Responses in Tomato VasculatureAaron J. Ogden0Jishnu J. Bhatt1Heather M. Brewer2Jack Kintigh3Samwel M. Kariuki4Sairam Rudrabhatla5Joshua N. Adkins6Wayne R. Curtis7Earth and Biological Sciences Directorate, Pacific Northwest National Laboratories, 902 Battelle Blvd, Richland, WA 99301, USAPlant Biology Graduate Program, The Pennsylvania State University, University Park, PA 16802, USAEarth and Biological Sciences Directorate, Pacific Northwest National Laboratories, 902 Battelle Blvd, Richland, WA 99301, USADepartment of Chemical Engineering, The Pennsylvania State University, University Park, PA 16802, USADepartment of Chemical Engineering, The Pennsylvania State University, University Park, PA 16802, USASchool of Science, Engineering, and Technology, The Pennsylvania State University, Harrisburg Campus, 777 W Harrisburg Pike, Middletown, PA 17057, USAEarth and Biological Sciences Directorate, Pacific Northwest National Laboratories, 902 Battelle Blvd, Richland, WA 99301, USAPlant Biology Graduate Program, The Pennsylvania State University, University Park, PA 16802, USADrought is the leading cause of agricultural yield loss among all abiotic stresses, and the link between water deficit and phloem protein contents is relatively unexplored. Here we collected phloem exudates from <i>Solanum lycopersicum</i> leaves during periods of drought stress and recovery. Our analysis identified 2558 proteins, the most abundant of which were previously localized to the phloem. Independent of drought, enrichment analysis of the total phloem exudate protein profiles from all samples suggests that the protein content of phloem sap is complex, and includes proteins that function in chaperone systems, branched-chain amino acid synthesis, trehalose metabolism, and RNA silencing. We observed 169 proteins whose abundance changed significantly within the phloem sap, either during drought or recovery. Proteins that became significantly more abundant during drought include members of lipid metabolism, chaperone-mediated protein folding, carboxylic acid metabolism, abscisic acid signaling, cytokinin biosynthesis, and amino acid metabolism. Conversely, proteins involved in lipid signaling, sphingolipid metabolism, cell wall organization, carbohydrate metabolism, and a mitogen-activated protein kinase are decreased during drought. Our experiment has achieved an in-depth profiling of phloem sap protein contents during drought stress and recovery that supports previous findings and provides new evidence that multiple biological processes are involved in drought adaptation.https://www.mdpi.com/1422-0067/21/12/4461phloem exudateproteomicsdroughtabiotic stress |
| spellingShingle | Aaron J. Ogden Jishnu J. Bhatt Heather M. Brewer Jack Kintigh Samwel M. Kariuki Sairam Rudrabhatla Joshua N. Adkins Wayne R. Curtis Phloem Exudate Protein Profiles during Drought and Recovery Reveal Abiotic Stress Responses in Tomato Vasculature International Journal of Molecular Sciences phloem exudate proteomics drought abiotic stress |
| title | Phloem Exudate Protein Profiles during Drought and Recovery Reveal Abiotic Stress Responses in Tomato Vasculature |
| title_full | Phloem Exudate Protein Profiles during Drought and Recovery Reveal Abiotic Stress Responses in Tomato Vasculature |
| title_fullStr | Phloem Exudate Protein Profiles during Drought and Recovery Reveal Abiotic Stress Responses in Tomato Vasculature |
| title_full_unstemmed | Phloem Exudate Protein Profiles during Drought and Recovery Reveal Abiotic Stress Responses in Tomato Vasculature |
| title_short | Phloem Exudate Protein Profiles during Drought and Recovery Reveal Abiotic Stress Responses in Tomato Vasculature |
| title_sort | phloem exudate protein profiles during drought and recovery reveal abiotic stress responses in tomato vasculature |
| topic | phloem exudate proteomics drought abiotic stress |
| url | https://www.mdpi.com/1422-0067/21/12/4461 |
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