Improved glucose tolerance in acyl CoA:diacylglycerol acyltransferase 1-null mice is dependent on diet
<p>Abstract</p> <p>Background</p> <p>Mice that lack acyl CoA:diacylglycerol acyltransferase (<it>Dgat1</it><sup>-/- </sup>mice) are reported to have a reduced body fat content and improved glucose tolerance and insulin sensitivity. Studies so far...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2007-01-01
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| Series: | Lipids in Health and Disease |
| Online Access: | http://www.lipidworld.com/content/6/1/2 |
| _version_ | 1811260326691209216 |
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| author | Cawthorne Michael A O'Dowd Jacqueline Cornick Claire Wang Steven JY Arch Jonathan RS |
| author_facet | Cawthorne Michael A O'Dowd Jacqueline Cornick Claire Wang Steven JY Arch Jonathan RS |
| author_sort | Cawthorne Michael A |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Mice that lack acyl CoA:diacylglycerol acyltransferase (<it>Dgat1</it><sup>-/- </sup>mice) are reported to have a reduced body fat content and improved glucose tolerance and insulin sensitivity. Studies so far have focussed on male null mice fed a high fat diet and there are few data on heterozygotes. We compared male and female <it>Dgat1</it><sup>-/-</sup>, <it>Dgat1</it><sup>+/- </sup>and <it>Dgat1</it><sup>+/+ </sup>C57Bl/6 mice fed on either standard chow or a high fat diet.</p> <p>Results</p> <p>Body fat content was lower in the <it>Dgat1</it><sup>-/- </sup>than the <it>Dgat1</it><sup>+/+ </sup>mice in both experiments; lean body mass was higher in male <it>Dgat1</it><sup>-/- </sup>than <it>Dgat1</it><sup>+/+ </sup>mice fed on the high fat diet. Energy intake and expenditure were higher in male <it>Dgat1</it><sup>-/- </sup>than <it>Dgat1</it><sup>+/+ </sup>mice; these differences were less marked or absent in females. The body fat content of female <it>Dgat1</it><sup>+/- </sup>mice was intermediate between that of <it>Dgat1</it><sup>-/- </sup>and <it>Dgat1</it><sup>+/+ </sup>mice, whereas male Dgat1<sup>+/- </sup>mice were similar to or fatter than <it>Dgat1</it><sup>+/+ </sup>mice. Glucose tolerance was improved and plasma insulin reduced in <it>Dgat1</it><sup>-/- </sup>mice fed on the high fat diet, but not on the chow diet. Both male and female <it>Dgat1</it><sup>+/- </sup>mice had similar glucose tolerance to <it>Dgat1</it><sup>+/+ </sup>mice.</p> <p>Conclusion</p> <p>These results suggest that although ablation of DGAT1 improves glucose tolerance by preventing obesity in mice fed on a high fat diet, it does not improve glucose tolerance in mice fed on a low fat diet.</p> |
| first_indexed | 2024-04-12T18:45:47Z |
| format | Article |
| id | doaj.art-effb900c25d24926bb309e3a6aef6f5c |
| institution | Directory Open Access Journal |
| issn | 1476-511X |
| language | English |
| last_indexed | 2024-04-12T18:45:47Z |
| publishDate | 2007-01-01 |
| publisher | BMC |
| record_format | Article |
| series | Lipids in Health and Disease |
| spelling | doaj.art-effb900c25d24926bb309e3a6aef6f5c2022-12-22T03:20:37ZengBMCLipids in Health and Disease1476-511X2007-01-0161210.1186/1476-511X-6-2Improved glucose tolerance in acyl CoA:diacylglycerol acyltransferase 1-null mice is dependent on dietCawthorne Michael AO'Dowd JacquelineCornick ClaireWang Steven JYArch Jonathan RS<p>Abstract</p> <p>Background</p> <p>Mice that lack acyl CoA:diacylglycerol acyltransferase (<it>Dgat1</it><sup>-/- </sup>mice) are reported to have a reduced body fat content and improved glucose tolerance and insulin sensitivity. Studies so far have focussed on male null mice fed a high fat diet and there are few data on heterozygotes. We compared male and female <it>Dgat1</it><sup>-/-</sup>, <it>Dgat1</it><sup>+/- </sup>and <it>Dgat1</it><sup>+/+ </sup>C57Bl/6 mice fed on either standard chow or a high fat diet.</p> <p>Results</p> <p>Body fat content was lower in the <it>Dgat1</it><sup>-/- </sup>than the <it>Dgat1</it><sup>+/+ </sup>mice in both experiments; lean body mass was higher in male <it>Dgat1</it><sup>-/- </sup>than <it>Dgat1</it><sup>+/+ </sup>mice fed on the high fat diet. Energy intake and expenditure were higher in male <it>Dgat1</it><sup>-/- </sup>than <it>Dgat1</it><sup>+/+ </sup>mice; these differences were less marked or absent in females. The body fat content of female <it>Dgat1</it><sup>+/- </sup>mice was intermediate between that of <it>Dgat1</it><sup>-/- </sup>and <it>Dgat1</it><sup>+/+ </sup>mice, whereas male Dgat1<sup>+/- </sup>mice were similar to or fatter than <it>Dgat1</it><sup>+/+ </sup>mice. Glucose tolerance was improved and plasma insulin reduced in <it>Dgat1</it><sup>-/- </sup>mice fed on the high fat diet, but not on the chow diet. Both male and female <it>Dgat1</it><sup>+/- </sup>mice had similar glucose tolerance to <it>Dgat1</it><sup>+/+ </sup>mice.</p> <p>Conclusion</p> <p>These results suggest that although ablation of DGAT1 improves glucose tolerance by preventing obesity in mice fed on a high fat diet, it does not improve glucose tolerance in mice fed on a low fat diet.</p>http://www.lipidworld.com/content/6/1/2 |
| spellingShingle | Cawthorne Michael A O'Dowd Jacqueline Cornick Claire Wang Steven JY Arch Jonathan RS Improved glucose tolerance in acyl CoA:diacylglycerol acyltransferase 1-null mice is dependent on diet Lipids in Health and Disease |
| title | Improved glucose tolerance in acyl CoA:diacylglycerol acyltransferase 1-null mice is dependent on diet |
| title_full | Improved glucose tolerance in acyl CoA:diacylglycerol acyltransferase 1-null mice is dependent on diet |
| title_fullStr | Improved glucose tolerance in acyl CoA:diacylglycerol acyltransferase 1-null mice is dependent on diet |
| title_full_unstemmed | Improved glucose tolerance in acyl CoA:diacylglycerol acyltransferase 1-null mice is dependent on diet |
| title_short | Improved glucose tolerance in acyl CoA:diacylglycerol acyltransferase 1-null mice is dependent on diet |
| title_sort | improved glucose tolerance in acyl coa diacylglycerol acyltransferase 1 null mice is dependent on diet |
| url | http://www.lipidworld.com/content/6/1/2 |
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