Characterization of VvmiR166s-Target Modules and Their Interaction Pathways in Modulation of Gibberellic-Acid-Induced Grape Seedless Berries
Exogenous GA is widely used to efficiently induce grape seedless berry development for significantly improving berry quality. Recently, we found that VvmiR166s are important regulators of response to GA in grapes, but its roles in GA-induced seedless grape berry development remain elusive. Here, the...
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| Format: | Article |
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MDPI AG
2023-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/22/16279 |
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| author | Yunhe Bai Zhuangwei Wang Linjia Luo Xuxian Xuan Wei Tang Ziyang Qu Tianyu Dong Ziyang Qi Mucheng Yu Weimin Wu Jinggui Fang Chen Wang |
| author_facet | Yunhe Bai Zhuangwei Wang Linjia Luo Xuxian Xuan Wei Tang Ziyang Qu Tianyu Dong Ziyang Qi Mucheng Yu Weimin Wu Jinggui Fang Chen Wang |
| author_sort | Yunhe Bai |
| collection | DOAJ |
| description | Exogenous GA is widely used to efficiently induce grape seedless berry development for significantly improving berry quality. Recently, we found that VvmiR166s are important regulators of response to GA in grapes, but its roles in GA-induced seedless grape berry development remain elusive. Here, the precise sequences of VvmiR166s and their targets <i>VvREV</i>, <i>VvHB15</i> and <i>VvHOX32</i> were determined in grape cv. ‘<i>Rosario Bianco</i>’, and the cleavage interactions of VvmiR166s-<i>VvHB15/VvHOX32/VvREV</i> modules and the variations in their cleavage roles were confirmed in grape berries. Exogenous GA treatment significantly induced a change in their expression correlations from positive to negative between VvmiR166s and their target genes at the seeds during the stone-hardening stages (32 DAF–46 DAF) in grape berries, indicating exogenous GA change action modes of VvmiR166s on their targets in this process, in which exogenous GA mainly enhanced the negative regulatory roles of VvmiR166s on <i>VvHB15</i> among all three VvmiR166s-target pairs. The transient OE-VvmiR166a-h/OE-VvHB15 in tobacco confirmed that out of the VvmiR166 family, VvmiR166h/a/b might be the main factors in modulating lignin synthesis through inhibiting <i>VvHB15</i>, of which VvmiR166h-<i>VvHB15</i>-<i>NtPAL4/NtCCR1/NtCCR2/NtCCoAMT5/NtCOMT1</i> and VvmiR166a/b-<i>VvHB15</i>-<i>NtCAD1</i> are the potential key regulatory modules in lignin synthesis. Together with the GA-induced expression modes of VvmiR166s-<i>VvHB15</i> and genes related to lignin synthesis in grape berries, we revealed that GA might repress lignin synthesis mainly by repressing <i>VvCAD1/VvCCR2/VvPAL2/VvPAL3/Vv4CL/VvLac7</i> levels via mediating VvmiR166s-<i>VvHB15</i> modules in GA-induced grape seedless berries. Our findings present a novel insight into the roles of VvmiR66s that are responsive to GA in repressing the lignin synthesis of grape seedless berries, with different lignin-synthesis-enzyme-dependent action pathways in diverse plants, which have important implications for the molecular breeding of high-quality seedless grape berries. |
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| language | English |
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| spelling | doaj.art-effe9acc039541fd845bf6586f2521132023-11-24T14:46:58ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-11-0124221627910.3390/ijms242216279Characterization of VvmiR166s-Target Modules and Their Interaction Pathways in Modulation of Gibberellic-Acid-Induced Grape Seedless BerriesYunhe Bai0Zhuangwei Wang1Linjia Luo2Xuxian Xuan3Wei Tang4Ziyang Qu5Tianyu Dong6Ziyang Qi7Mucheng Yu8Weimin Wu9Jinggui Fang10Chen Wang11College of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaJiangsu Academy of Agricultural Sciences, Institute of Pomology, Nanjing 210014, ChinaCollege of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaJiangsu Academy of Agricultural Sciences, Institute of Pomology, Nanjing 210014, ChinaCollege of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaCollege of Horticulture, Nanjing Agricultural University, Nanjing 210095, ChinaExogenous GA is widely used to efficiently induce grape seedless berry development for significantly improving berry quality. Recently, we found that VvmiR166s are important regulators of response to GA in grapes, but its roles in GA-induced seedless grape berry development remain elusive. Here, the precise sequences of VvmiR166s and their targets <i>VvREV</i>, <i>VvHB15</i> and <i>VvHOX32</i> were determined in grape cv. ‘<i>Rosario Bianco</i>’, and the cleavage interactions of VvmiR166s-<i>VvHB15/VvHOX32/VvREV</i> modules and the variations in their cleavage roles were confirmed in grape berries. Exogenous GA treatment significantly induced a change in their expression correlations from positive to negative between VvmiR166s and their target genes at the seeds during the stone-hardening stages (32 DAF–46 DAF) in grape berries, indicating exogenous GA change action modes of VvmiR166s on their targets in this process, in which exogenous GA mainly enhanced the negative regulatory roles of VvmiR166s on <i>VvHB15</i> among all three VvmiR166s-target pairs. The transient OE-VvmiR166a-h/OE-VvHB15 in tobacco confirmed that out of the VvmiR166 family, VvmiR166h/a/b might be the main factors in modulating lignin synthesis through inhibiting <i>VvHB15</i>, of which VvmiR166h-<i>VvHB15</i>-<i>NtPAL4/NtCCR1/NtCCR2/NtCCoAMT5/NtCOMT1</i> and VvmiR166a/b-<i>VvHB15</i>-<i>NtCAD1</i> are the potential key regulatory modules in lignin synthesis. Together with the GA-induced expression modes of VvmiR166s-<i>VvHB15</i> and genes related to lignin synthesis in grape berries, we revealed that GA might repress lignin synthesis mainly by repressing <i>VvCAD1/VvCCR2/VvPAL2/VvPAL3/Vv4CL/VvLac7</i> levels via mediating VvmiR166s-<i>VvHB15</i> modules in GA-induced grape seedless berries. Our findings present a novel insight into the roles of VvmiR66s that are responsive to GA in repressing the lignin synthesis of grape seedless berries, with different lignin-synthesis-enzyme-dependent action pathways in diverse plants, which have important implications for the molecular breeding of high-quality seedless grape berries.https://www.mdpi.com/1422-0067/24/22/16279seedless grapegibberellinVvmiR166sHD-Zip IIIlignin |
| spellingShingle | Yunhe Bai Zhuangwei Wang Linjia Luo Xuxian Xuan Wei Tang Ziyang Qu Tianyu Dong Ziyang Qi Mucheng Yu Weimin Wu Jinggui Fang Chen Wang Characterization of VvmiR166s-Target Modules and Their Interaction Pathways in Modulation of Gibberellic-Acid-Induced Grape Seedless Berries International Journal of Molecular Sciences seedless grape gibberellin VvmiR166s HD-Zip III lignin |
| title | Characterization of VvmiR166s-Target Modules and Their Interaction Pathways in Modulation of Gibberellic-Acid-Induced Grape Seedless Berries |
| title_full | Characterization of VvmiR166s-Target Modules and Their Interaction Pathways in Modulation of Gibberellic-Acid-Induced Grape Seedless Berries |
| title_fullStr | Characterization of VvmiR166s-Target Modules and Their Interaction Pathways in Modulation of Gibberellic-Acid-Induced Grape Seedless Berries |
| title_full_unstemmed | Characterization of VvmiR166s-Target Modules and Their Interaction Pathways in Modulation of Gibberellic-Acid-Induced Grape Seedless Berries |
| title_short | Characterization of VvmiR166s-Target Modules and Their Interaction Pathways in Modulation of Gibberellic-Acid-Induced Grape Seedless Berries |
| title_sort | characterization of vvmir166s target modules and their interaction pathways in modulation of gibberellic acid induced grape seedless berries |
| topic | seedless grape gibberellin VvmiR166s HD-Zip III lignin |
| url | https://www.mdpi.com/1422-0067/24/22/16279 |
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