Developmental Validation of the Novel Five-Dye-Labeled Multiplex Autosomal STR Panel and Its Forensic Efficiency Evaluation
Short tandem repeats (STRs) are the most frequently used genetic markers in forensic genetics due to their high genetic diversities and abundant distributions in the human genome. Currently, the combined DNA index system is commonly incorporated into various commercial kits for forensic research. So...
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| Format: | Article |
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Frontiers Media S.A.
2022-05-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fgene.2022.897650/full |
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| author | Shimei Huang Xiaoye Jin Hongling Zhang Haiying Jin Zheng Ren Qiyan Wang Yubo Liu Jingyan Ji Meiqing Yang Han Zhang Xingkai Zheng Danlu Song Bingjie Zheng Jiang Huang |
| author_facet | Shimei Huang Xiaoye Jin Hongling Zhang Haiying Jin Zheng Ren Qiyan Wang Yubo Liu Jingyan Ji Meiqing Yang Han Zhang Xingkai Zheng Danlu Song Bingjie Zheng Jiang Huang |
| author_sort | Shimei Huang |
| collection | DOAJ |
| description | Short tandem repeats (STRs) are the most frequently used genetic markers in forensic genetics due to their high genetic diversities and abundant distributions in the human genome. Currently, the combined DNA index system is commonly incorporated into various commercial kits for forensic research. Some novel STRs that are different from the combined DNA index system were not only used to assess complex paternity cases but also could provide more genetic information and higher forensic efficiency in combination with those commonly used STRs. In this study, we validated forensic performance of a novel multiplex amplification STR panel to evaluate its sensitivity, species specificity, forensic application values, and so on. Obtained results revealed that the kit showed high sensitivity, and the complete allelic profile could be observed at 0.125 ng DNA sample. In addition, the kit possessed high species specificity, good tolerance to common inhibitors, and accurate genotyping ability. More importantly, STRs out of the kit displayed high discrimination power and probability of exclusion. To sum up, the novel kit presented in this study can be viewed as a promising tool for forensic human identification and complex paternity analysis. |
| first_indexed | 2024-12-12T13:02:34Z |
| format | Article |
| id | doaj.art-efff4017016d4b99a37c94be21b41863 |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-12T13:02:34Z |
| publishDate | 2022-05-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Genetics |
| spelling | doaj.art-efff4017016d4b99a37c94be21b418632022-12-22T00:23:45ZengFrontiers Media S.A.Frontiers in Genetics1664-80212022-05-011310.3389/fgene.2022.897650897650Developmental Validation of the Novel Five-Dye-Labeled Multiplex Autosomal STR Panel and Its Forensic Efficiency EvaluationShimei Huang0Xiaoye Jin1Hongling Zhang2Haiying Jin3Zheng Ren4Qiyan Wang5Yubo Liu6Jingyan Ji7Meiqing Yang8Han Zhang9Xingkai Zheng10Danlu Song11Bingjie Zheng12Jiang Huang13Department of Forensic Medicine, Guizhou Medical University, Guiyang, ChinaDepartment of Forensic Medicine, Guizhou Medical University, Guiyang, ChinaDepartment of Forensic Medicine, Guizhou Medical University, Guiyang, ChinaNingbo Health Gene Technologies Co.,Ltd, Ningbo, ChinaDepartment of Forensic Medicine, Guizhou Medical University, Guiyang, ChinaDepartment of Forensic Medicine, Guizhou Medical University, Guiyang, ChinaDepartment of Forensic Medicine, Guizhou Medical University, Guiyang, ChinaDepartment of Forensic Medicine, Guizhou Medical University, Guiyang, ChinaDepartment of Forensic Medicine, Guizhou Medical University, Guiyang, ChinaDepartment of Forensic Medicine, Guizhou Medical University, Guiyang, ChinaNingbo Health Gene Technologies Co.,Ltd, Ningbo, ChinaNingbo Health Gene Technologies Co.,Ltd, Ningbo, ChinaNingbo Health Gene Technologies Co.,Ltd, Ningbo, ChinaDepartment of Forensic Medicine, Guizhou Medical University, Guiyang, ChinaShort tandem repeats (STRs) are the most frequently used genetic markers in forensic genetics due to their high genetic diversities and abundant distributions in the human genome. Currently, the combined DNA index system is commonly incorporated into various commercial kits for forensic research. Some novel STRs that are different from the combined DNA index system were not only used to assess complex paternity cases but also could provide more genetic information and higher forensic efficiency in combination with those commonly used STRs. In this study, we validated forensic performance of a novel multiplex amplification STR panel to evaluate its sensitivity, species specificity, forensic application values, and so on. Obtained results revealed that the kit showed high sensitivity, and the complete allelic profile could be observed at 0.125 ng DNA sample. In addition, the kit possessed high species specificity, good tolerance to common inhibitors, and accurate genotyping ability. More importantly, STRs out of the kit displayed high discrimination power and probability of exclusion. To sum up, the novel kit presented in this study can be viewed as a promising tool for forensic human identification and complex paternity analysis.https://www.frontiersin.org/articles/10.3389/fgene.2022.897650/fullSTRsCODISforensic researchdevelopmental validationcomplex paternity analysis |
| spellingShingle | Shimei Huang Xiaoye Jin Hongling Zhang Haiying Jin Zheng Ren Qiyan Wang Yubo Liu Jingyan Ji Meiqing Yang Han Zhang Xingkai Zheng Danlu Song Bingjie Zheng Jiang Huang Developmental Validation of the Novel Five-Dye-Labeled Multiplex Autosomal STR Panel and Its Forensic Efficiency Evaluation Frontiers in Genetics STRs CODIS forensic research developmental validation complex paternity analysis |
| title | Developmental Validation of the Novel Five-Dye-Labeled Multiplex Autosomal STR Panel and Its Forensic Efficiency Evaluation |
| title_full | Developmental Validation of the Novel Five-Dye-Labeled Multiplex Autosomal STR Panel and Its Forensic Efficiency Evaluation |
| title_fullStr | Developmental Validation of the Novel Five-Dye-Labeled Multiplex Autosomal STR Panel and Its Forensic Efficiency Evaluation |
| title_full_unstemmed | Developmental Validation of the Novel Five-Dye-Labeled Multiplex Autosomal STR Panel and Its Forensic Efficiency Evaluation |
| title_short | Developmental Validation of the Novel Five-Dye-Labeled Multiplex Autosomal STR Panel and Its Forensic Efficiency Evaluation |
| title_sort | developmental validation of the novel five dye labeled multiplex autosomal str panel and its forensic efficiency evaluation |
| topic | STRs CODIS forensic research developmental validation complex paternity analysis |
| url | https://www.frontiersin.org/articles/10.3389/fgene.2022.897650/full |
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