Cellular correlates of gray matter volume changes in magnetic resonance morphometry identified by two-photon microscopy
Abstract Magnetic resonance imaging (MRI) of the brain combined with voxel-based morphometry (VBM) revealed changes in gray matter volume (GMV) in various disorders. However, the cellular basis of GMV changes has remained largely unclear. We correlated changes in GMV with cellular metrics by imaging...
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| Format: | Article |
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Nature Portfolio
2021-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-83491-8 |
| _version_ | 1818982378818764800 |
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| author | Livia Asan Claudia Falfán-Melgoza Carlo A. Beretta Markus Sack Lei Zheng Wolfgang Weber-Fahr Thomas Kuner Johannes Knabbe |
| author_facet | Livia Asan Claudia Falfán-Melgoza Carlo A. Beretta Markus Sack Lei Zheng Wolfgang Weber-Fahr Thomas Kuner Johannes Knabbe |
| author_sort | Livia Asan |
| collection | DOAJ |
| description | Abstract Magnetic resonance imaging (MRI) of the brain combined with voxel-based morphometry (VBM) revealed changes in gray matter volume (GMV) in various disorders. However, the cellular basis of GMV changes has remained largely unclear. We correlated changes in GMV with cellular metrics by imaging mice with MRI and two-photon in vivo microscopy at three time points within 12 weeks, taking advantage of age-dependent changes in brain structure. Imaging fluorescent cell nuclei allowed inferences on (i) physical tissue volume as determined from reference spaces outlined by nuclei, (ii) cell density, (iii) the extent of cell clustering, and (iv) the volume of cell nuclei. Our data indicate that physical tissue volume alterations only account for 13.0% of the variance in GMV change. However, when including comprehensive measurements of nucleus volume and cell density, 35.6% of the GMV variance could be explained, highlighting the influence of distinct cellular mechanisms on VBM results. |
| first_indexed | 2024-12-20T17:46:16Z |
| format | Article |
| id | doaj.art-efff63cb8c634002b9771ee8ae1f390e |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-20T17:46:16Z |
| publishDate | 2021-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-efff63cb8c634002b9771ee8ae1f390e2022-12-21T19:30:58ZengNature PortfolioScientific Reports2045-23222021-02-0111112010.1038/s41598-021-83491-8Cellular correlates of gray matter volume changes in magnetic resonance morphometry identified by two-photon microscopyLivia Asan0Claudia Falfán-Melgoza1Carlo A. Beretta2Markus Sack3Lei Zheng4Wolfgang Weber-Fahr5Thomas Kuner6Johannes Knabbe7Department of Functional Neuroanatomy, Institute for Anatomy and Cell Biology, Heidelberg UniversityTranslational Imaging Research Group, Central Institute of Mental Health, Medical Faculty of Mannheim, Heidelberg UniversityDepartment of Functional Neuroanatomy, Institute for Anatomy and Cell Biology, Heidelberg UniversityTranslational Imaging Research Group, Central Institute of Mental Health, Medical Faculty of Mannheim, Heidelberg UniversityTranslational Imaging Research Group, Central Institute of Mental Health, Medical Faculty of Mannheim, Heidelberg UniversityTranslational Imaging Research Group, Central Institute of Mental Health, Medical Faculty of Mannheim, Heidelberg UniversityDepartment of Functional Neuroanatomy, Institute for Anatomy and Cell Biology, Heidelberg UniversityDepartment of Functional Neuroanatomy, Institute for Anatomy and Cell Biology, Heidelberg UniversityAbstract Magnetic resonance imaging (MRI) of the brain combined with voxel-based morphometry (VBM) revealed changes in gray matter volume (GMV) in various disorders. However, the cellular basis of GMV changes has remained largely unclear. We correlated changes in GMV with cellular metrics by imaging mice with MRI and two-photon in vivo microscopy at three time points within 12 weeks, taking advantage of age-dependent changes in brain structure. Imaging fluorescent cell nuclei allowed inferences on (i) physical tissue volume as determined from reference spaces outlined by nuclei, (ii) cell density, (iii) the extent of cell clustering, and (iv) the volume of cell nuclei. Our data indicate that physical tissue volume alterations only account for 13.0% of the variance in GMV change. However, when including comprehensive measurements of nucleus volume and cell density, 35.6% of the GMV variance could be explained, highlighting the influence of distinct cellular mechanisms on VBM results.https://doi.org/10.1038/s41598-021-83491-8 |
| spellingShingle | Livia Asan Claudia Falfán-Melgoza Carlo A. Beretta Markus Sack Lei Zheng Wolfgang Weber-Fahr Thomas Kuner Johannes Knabbe Cellular correlates of gray matter volume changes in magnetic resonance morphometry identified by two-photon microscopy Scientific Reports |
| title | Cellular correlates of gray matter volume changes in magnetic resonance morphometry identified by two-photon microscopy |
| title_full | Cellular correlates of gray matter volume changes in magnetic resonance morphometry identified by two-photon microscopy |
| title_fullStr | Cellular correlates of gray matter volume changes in magnetic resonance morphometry identified by two-photon microscopy |
| title_full_unstemmed | Cellular correlates of gray matter volume changes in magnetic resonance morphometry identified by two-photon microscopy |
| title_short | Cellular correlates of gray matter volume changes in magnetic resonance morphometry identified by two-photon microscopy |
| title_sort | cellular correlates of gray matter volume changes in magnetic resonance morphometry identified by two photon microscopy |
| url | https://doi.org/10.1038/s41598-021-83491-8 |
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