Sub-2 Å Ewald curvature corrected structure of an AAV2 capsid variant
Single-particle cryo-EM is a powerful method for macromolecular structure determination. Here the authors demonstrate that Ewald sphere curvature correction, sub-Angstrom pixilation and per-particle CTF refinement can improve map quality and resolution and present the 1.86 Å cryo-EM structure of an...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2018-09-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/s41467-018-06076-6 |
| _version_ | 1818716810321592320 |
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| author | Yong Zi Tan Sriram Aiyer Mario Mietzsch Joshua A. Hull Robert McKenna Joshua Grieger R. Jude Samulski Timothy S. Baker Mavis Agbandje-McKenna Dmitry Lyumkis |
| author_facet | Yong Zi Tan Sriram Aiyer Mario Mietzsch Joshua A. Hull Robert McKenna Joshua Grieger R. Jude Samulski Timothy S. Baker Mavis Agbandje-McKenna Dmitry Lyumkis |
| author_sort | Yong Zi Tan |
| collection | DOAJ |
| description | Single-particle cryo-EM is a powerful method for macromolecular structure determination. Here the authors demonstrate that Ewald sphere curvature correction, sub-Angstrom pixilation and per-particle CTF refinement can improve map quality and resolution and present the 1.86 Å cryo-EM structure of an adeno-associated virus serotype 2 variant. |
| first_indexed | 2024-12-17T19:25:10Z |
| format | Article |
| id | doaj.art-f000b02747a14526ae63d8ed17d8476c |
| institution | Directory Open Access Journal |
| issn | 2041-1723 |
| language | English |
| last_indexed | 2024-12-17T19:25:10Z |
| publishDate | 2018-09-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Nature Communications |
| spelling | doaj.art-f000b02747a14526ae63d8ed17d8476c2022-12-21T21:35:24ZengNature PortfolioNature Communications2041-17232018-09-019111110.1038/s41467-018-06076-6Sub-2 Å Ewald curvature corrected structure of an AAV2 capsid variantYong Zi Tan0Sriram Aiyer1Mario Mietzsch2Joshua A. Hull3Robert McKenna4Joshua Grieger5R. Jude Samulski6Timothy S. Baker7Mavis Agbandje-McKenna8Dmitry Lyumkis9The National Resource for Automated Molecular Microscopy, Simons Electron Microscopy Center, New York Structural Biology CenterLaboratory of Genetics and Helmsley Center for Genomic Medicine, The Salk Institute for Biological StudiesDepartment of Biochemistry and Molecular Biology, Center for Structural Biology, The McKnight Brain Institute, College of Medicine, University of FloridaDepartment of Biochemistry and Molecular Biology, Center for Structural Biology, The McKnight Brain Institute, College of Medicine, University of FloridaDepartment of Biochemistry and Molecular Biology, Center for Structural Biology, The McKnight Brain Institute, College of Medicine, University of FloridaGene Therapy Center, University of North Carolina at Chapel HillGene Therapy Center, University of North Carolina at Chapel HillDepartment of Chemistry and Biochemistry and Division of Biological Sciences, University of California-San DiegoDepartment of Biochemistry and Molecular Biology, Center for Structural Biology, The McKnight Brain Institute, College of Medicine, University of FloridaLaboratory of Genetics and Helmsley Center for Genomic Medicine, The Salk Institute for Biological StudiesSingle-particle cryo-EM is a powerful method for macromolecular structure determination. Here the authors demonstrate that Ewald sphere curvature correction, sub-Angstrom pixilation and per-particle CTF refinement can improve map quality and resolution and present the 1.86 Å cryo-EM structure of an adeno-associated virus serotype 2 variant.https://doi.org/10.1038/s41467-018-06076-6 |
| spellingShingle | Yong Zi Tan Sriram Aiyer Mario Mietzsch Joshua A. Hull Robert McKenna Joshua Grieger R. Jude Samulski Timothy S. Baker Mavis Agbandje-McKenna Dmitry Lyumkis Sub-2 Å Ewald curvature corrected structure of an AAV2 capsid variant Nature Communications |
| title | Sub-2 Å Ewald curvature corrected structure of an AAV2 capsid variant |
| title_full | Sub-2 Å Ewald curvature corrected structure of an AAV2 capsid variant |
| title_fullStr | Sub-2 Å Ewald curvature corrected structure of an AAV2 capsid variant |
| title_full_unstemmed | Sub-2 Å Ewald curvature corrected structure of an AAV2 capsid variant |
| title_short | Sub-2 Å Ewald curvature corrected structure of an AAV2 capsid variant |
| title_sort | sub 2 a ewald curvature corrected structure of an aav2 capsid variant |
| url | https://doi.org/10.1038/s41467-018-06076-6 |
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