Study on the protective effect of berberine treatment on sepsis based on gut microbiota and metabolomic analysis
IntroductionSepsis, an infection with multiorgan dysfunction, is a serious burden on human health. Berberine (BBR), a bioactive component, has a protective effect on sepsis and the effect may be related to gut microbiota. However, studies on the role of BBR with gut microbiota in sepsis are lacking....
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-12-01
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| Series: | Frontiers in Nutrition |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2022.1049106/full |
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| author | Huibin Pan Lixia Huo Weiyun Shen Zhuquan Dai Ying Bao Chaohui Ji Jie Zhang |
| author_facet | Huibin Pan Lixia Huo Weiyun Shen Zhuquan Dai Ying Bao Chaohui Ji Jie Zhang |
| author_sort | Huibin Pan |
| collection | DOAJ |
| description | IntroductionSepsis, an infection with multiorgan dysfunction, is a serious burden on human health. Berberine (BBR), a bioactive component, has a protective effect on sepsis and the effect may be related to gut microbiota. However, studies on the role of BBR with gut microbiota in sepsis are lacking. Therefore, this study investigated the ameliorative effects and the underlying mechanisms of BBR on cecal ligature and puncture (CLP) rats.MethodsThis study has observed the effect of BBR on pathological injury, Inflammation, intestinal barrier function, gut microbiota, and metabolite change in CLP rats by Hematoxylin-eosin staining, enzyme-linked immunosorbent assays, flow cytometry, 16S rDNA, and metabolomics analyses.ResultsThe inhibition effects of BBR treatment on the histological damage of the lung, kidney, and ileum, the interleukin (IL)-1b, IL-6, IL-17A, and monocyte chemokine-1 levels in serum in CLP rats were proved. Also, the BBR inhibited the diamine-oxidase and fluorescein isothiocyanate-dextran 40 levels, suggesting it can improve intestinal barrier function disorders. The cluster of differentiation (CD) 4+, CD8+, and CD25+ Forkhead box protein P3 (Foxp3) + T lymphocytes in splenocytes were up-regulated by BBR, while the IL-17A+CD4+ cell level was decreased. The abundance of gut microbiota in CLP rats was significantly different from that of the sham and BBR treatment rats. The significantly changed metabolites in the serum mainly included carbohydrates, phenols, benzoic acids, alcohols, vitamins et al. Additionally, this study predicted that the biological mechanism of BBR to ameliorate sepsis involves glycolysis-, nucleotide-, and amino acid-related metabolic pathways.DiscussionThis study proved the strong correlation between the improvement effect of BBR on sepsis and gut microbiota and analyzed by metabolomics that gut microbiota may improve CLP rats through metabolites, providing a scientific basis for BBR to improve sepsis and a new direction for the study of the biological mechanism. |
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| format | Article |
| id | doaj.art-f00152e4a4c244fb8b5f9073741bc4f5 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-04-12T01:00:40Z |
| publishDate | 2022-12-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-f00152e4a4c244fb8b5f9073741bc4f52022-12-22T03:54:29ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2022-12-01910.3389/fnut.2022.10491061049106Study on the protective effect of berberine treatment on sepsis based on gut microbiota and metabolomic analysisHuibin Pan0Lixia Huo1Weiyun Shen2Zhuquan Dai3Ying Bao4Chaohui Ji5Jie Zhang6Emergency Intensive Care Unit, The First Affiliated Hospital of Huzhou University, The First People's Hospital of Huzhou, Huzhou, Zhejiang, ChinaHuzhou Key Laboratory of Translational Medicine, The First Affiliated Hospital of Huzhou University, The First People's Hospital of Huzhou, Huzhou, Zhejiang, ChinaHuzhou Key Laboratory of Translational Medicine, The First Affiliated Hospital of Huzhou University, The First People's Hospital of Huzhou, Huzhou, Zhejiang, ChinaEmergency Intensive Care Unit, The First Affiliated Hospital of Huzhou University, The First People's Hospital of Huzhou, Huzhou, Zhejiang, ChinaDepartment of Surgery, The First Affiliated Hospital of Huzhou University, The First People's Hospital of Huzhou, Huzhou, Zhejiang, ChinaEmergency Intensive Care Unit, The First Affiliated Hospital of Huzhou University, The First People's Hospital of Huzhou, Huzhou, Zhejiang, ChinaEmergency Intensive Care Unit, The First Affiliated Hospital of Huzhou University, The First People's Hospital of Huzhou, Huzhou, Zhejiang, ChinaIntroductionSepsis, an infection with multiorgan dysfunction, is a serious burden on human health. Berberine (BBR), a bioactive component, has a protective effect on sepsis and the effect may be related to gut microbiota. However, studies on the role of BBR with gut microbiota in sepsis are lacking. Therefore, this study investigated the ameliorative effects and the underlying mechanisms of BBR on cecal ligature and puncture (CLP) rats.MethodsThis study has observed the effect of BBR on pathological injury, Inflammation, intestinal barrier function, gut microbiota, and metabolite change in CLP rats by Hematoxylin-eosin staining, enzyme-linked immunosorbent assays, flow cytometry, 16S rDNA, and metabolomics analyses.ResultsThe inhibition effects of BBR treatment on the histological damage of the lung, kidney, and ileum, the interleukin (IL)-1b, IL-6, IL-17A, and monocyte chemokine-1 levels in serum in CLP rats were proved. Also, the BBR inhibited the diamine-oxidase and fluorescein isothiocyanate-dextran 40 levels, suggesting it can improve intestinal barrier function disorders. The cluster of differentiation (CD) 4+, CD8+, and CD25+ Forkhead box protein P3 (Foxp3) + T lymphocytes in splenocytes were up-regulated by BBR, while the IL-17A+CD4+ cell level was decreased. The abundance of gut microbiota in CLP rats was significantly different from that of the sham and BBR treatment rats. The significantly changed metabolites in the serum mainly included carbohydrates, phenols, benzoic acids, alcohols, vitamins et al. Additionally, this study predicted that the biological mechanism of BBR to ameliorate sepsis involves glycolysis-, nucleotide-, and amino acid-related metabolic pathways.DiscussionThis study proved the strong correlation between the improvement effect of BBR on sepsis and gut microbiota and analyzed by metabolomics that gut microbiota may improve CLP rats through metabolites, providing a scientific basis for BBR to improve sepsis and a new direction for the study of the biological mechanism.https://www.frontiersin.org/articles/10.3389/fnut.2022.1049106/fullsepsisberberinegut microbiotametabolomics16S rDNA |
| spellingShingle | Huibin Pan Lixia Huo Weiyun Shen Zhuquan Dai Ying Bao Chaohui Ji Jie Zhang Study on the protective effect of berberine treatment on sepsis based on gut microbiota and metabolomic analysis Frontiers in Nutrition sepsis berberine gut microbiota metabolomics 16S rDNA |
| title | Study on the protective effect of berberine treatment on sepsis based on gut microbiota and metabolomic analysis |
| title_full | Study on the protective effect of berberine treatment on sepsis based on gut microbiota and metabolomic analysis |
| title_fullStr | Study on the protective effect of berberine treatment on sepsis based on gut microbiota and metabolomic analysis |
| title_full_unstemmed | Study on the protective effect of berberine treatment on sepsis based on gut microbiota and metabolomic analysis |
| title_short | Study on the protective effect of berberine treatment on sepsis based on gut microbiota and metabolomic analysis |
| title_sort | study on the protective effect of berberine treatment on sepsis based on gut microbiota and metabolomic analysis |
| topic | sepsis berberine gut microbiota metabolomics 16S rDNA |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2022.1049106/full |
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