In vitro antioxidant properties and antimicrobial activity of the ethanolic extract of Senecio nutans Sch. Beep. (Asteraceae)
Context: Senecio nutans Sch. Beep. is used by the Andean population of Ayacucho-Peru as traditional medicine for various health problems. Aims: To evaluate the antioxidant and antimicrobial activity of the ethanolic extract of leaves of S. nutans against the enteropathogen Escherichia coli and th...
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| Format: | Article |
| Language: | English |
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GarVal Editorial Ltda.
2022-11-01
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| Series: | Journal of Pharmacy & Pharmacognosy Research |
| Subjects: | |
| Online Access: | https://jppres.com/jppres/pdf/vol10/jppres22.1471_10.6.1026.pdf |
| _version_ | 1811187292811821056 |
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| author | Felipe Surco-Laos Jorge A. Garcia María R. Bendezú Doris Laos-Anchante Juan F. Panay-Centeno Manuel Valle-Campos Juan J. Palomino-Jhong Paulina Eliades Yarasca-Carlos Berta Loja Angel T. Alvarado |
| author_facet | Felipe Surco-Laos Jorge A. Garcia María R. Bendezú Doris Laos-Anchante Juan F. Panay-Centeno Manuel Valle-Campos Juan J. Palomino-Jhong Paulina Eliades Yarasca-Carlos Berta Loja Angel T. Alvarado |
| author_sort | Felipe Surco-Laos |
| collection | DOAJ |
| description | Context: Senecio nutans Sch. Beep. is used by the Andean population of Ayacucho-Peru as traditional medicine for various health problems.
Aims: To evaluate the antioxidant and antimicrobial activity of the ethanolic extract of leaves of S. nutans against the enteropathogen Escherichia coli and the filamentous fungus Aspergillus niger.
Methods: The antioxidant capacity of the ethanolic extract was evaluated by ABTS, DPPH, and FRAP assays; the antimicrobial activity was evaluated by the agar diffusion method, and determining the minimum inhibitory concentration (MIC), minimum bactericidal (MBC), and fungicidal concentration (MFC).
Results: Polyphenols, terpenes, and tannins were identified; the total polyphenolic content was 67.91 ± 0.29 mg GAE/g. The ethanolic extract at 20 mg/mL showed moderate antibacterial activity (79.14 ± 0.02% inhibition, MIC and MBC >400 μg/mL on E. coli), and antifungal (88.90 ± 0.17% inhibition, MIC and MCF >400 μg/mL on A. niger); radical scavenging capacity (ABTS 10.31 ± 0.09 mM TE/g; DPPH 8.28 ± 0.07 IC50 µg/mL) and iron reducing power (FRAP 17.72 ± 0.11 mM TE/g). S. nutans turned out to be a potential antimicrobial and antioxidant species associated with the presence of its bioactive components, for which further investigation is warranted.
Conclusions: The ethanolic extract of leaves S. nutans showed moderate activity against pathogenic bacteria (E. coli) and filamentous fungus (A. niger), as well as antioxidant activity in three in vitro methods. |
| first_indexed | 2024-04-11T13:59:50Z |
| format | Article |
| id | doaj.art-f00165a443de424baf15beced6cce6b9 |
| institution | Directory Open Access Journal |
| issn | 0719-4250 |
| language | English |
| last_indexed | 2024-04-11T13:59:50Z |
| publishDate | 2022-11-01 |
| publisher | GarVal Editorial Ltda. |
| record_format | Article |
| series | Journal of Pharmacy & Pharmacognosy Research |
| spelling | doaj.art-f00165a443de424baf15beced6cce6b92022-12-22T04:20:09ZengGarVal Editorial Ltda.Journal of Pharmacy & Pharmacognosy Research0719-42502022-11-0110610261036https://doi.org/10.56499/jppres22.1471_10.6.1026In vitro antioxidant properties and antimicrobial activity of the ethanolic extract of Senecio nutans Sch. Beep. (Asteraceae)Felipe Surco-Laos0Jorge A. Garcia1María R. Bendezú2Doris Laos-Anchante3Juan F. Panay-Centeno4Manuel Valle-Campos5 Juan J. Palomino-Jhong6Paulina Eliades Yarasca-Carlos7Berta Loja8Angel T. Alvarado9Faculty of Pharmacy and Biochemistry, San Luis Gonzaga National University of Ica, 11004, Ica, Peru.Faculty of Pharmacy and Biochemistry, San Luis Gonzaga National University of Ica, 11004, Ica, Peru.Faculty of Pharmacy and Biochemistry, San Luis Gonzaga National University of Ica, 11004, Ica, Peru.Faculty of Pharmacy and Biochemistry, San Luis Gonzaga National University of Ica, 11004, Ica, Peru.Faculty of Pharmacy and Biochemistry, San Luis Gonzaga National University of Ica, 11004, Ica, Peru.Faculty of Pharmacy and Biochemistry, San Luis Gonzaga National University of Ica, 11004, Ica, Peru.Faculty of Pharmacy and Biochemistry, San Luis Gonzaga National University of Ica, 11004, Ica, Peru.Biological Sciences Faculty, San Luis Gonzaga National University of Ica, 11004, Ica, Peru.Environmental Engineering, San Ignacio de Loyola University, La Molina 15024, Lima, Peru.International Research Network of Pharmacology and Precision Medicine (REDIFMEP), San Ignacio de Loyola University, La Molina 15024, Lima, Peru.Context: Senecio nutans Sch. Beep. is used by the Andean population of Ayacucho-Peru as traditional medicine for various health problems. Aims: To evaluate the antioxidant and antimicrobial activity of the ethanolic extract of leaves of S. nutans against the enteropathogen Escherichia coli and the filamentous fungus Aspergillus niger. Methods: The antioxidant capacity of the ethanolic extract was evaluated by ABTS, DPPH, and FRAP assays; the antimicrobial activity was evaluated by the agar diffusion method, and determining the minimum inhibitory concentration (MIC), minimum bactericidal (MBC), and fungicidal concentration (MFC). Results: Polyphenols, terpenes, and tannins were identified; the total polyphenolic content was 67.91 ± 0.29 mg GAE/g. The ethanolic extract at 20 mg/mL showed moderate antibacterial activity (79.14 ± 0.02% inhibition, MIC and MBC >400 μg/mL on E. coli), and antifungal (88.90 ± 0.17% inhibition, MIC and MCF >400 μg/mL on A. niger); radical scavenging capacity (ABTS 10.31 ± 0.09 mM TE/g; DPPH 8.28 ± 0.07 IC50 µg/mL) and iron reducing power (FRAP 17.72 ± 0.11 mM TE/g). S. nutans turned out to be a potential antimicrobial and antioxidant species associated with the presence of its bioactive components, for which further investigation is warranted. Conclusions: The ethanolic extract of leaves S. nutans showed moderate activity against pathogenic bacteria (E. coli) and filamentous fungus (A. niger), as well as antioxidant activity in three in vitro methods.https://jppres.com/jppres/pdf/vol10/jppres22.1471_10.6.1026.pdfantimicrobial activitychachacomain vitro antioxidant activityphenolic compoundssenecio nutans |
| spellingShingle | Felipe Surco-Laos Jorge A. Garcia María R. Bendezú Doris Laos-Anchante Juan F. Panay-Centeno Manuel Valle-Campos Juan J. Palomino-Jhong Paulina Eliades Yarasca-Carlos Berta Loja Angel T. Alvarado In vitro antioxidant properties and antimicrobial activity of the ethanolic extract of Senecio nutans Sch. Beep. (Asteraceae) Journal of Pharmacy & Pharmacognosy Research antimicrobial activity chachacoma in vitro antioxidant activity phenolic compounds senecio nutans |
| title | In vitro antioxidant properties and antimicrobial activity of the ethanolic extract of Senecio nutans Sch. Beep. (Asteraceae) |
| title_full | In vitro antioxidant properties and antimicrobial activity of the ethanolic extract of Senecio nutans Sch. Beep. (Asteraceae) |
| title_fullStr | In vitro antioxidant properties and antimicrobial activity of the ethanolic extract of Senecio nutans Sch. Beep. (Asteraceae) |
| title_full_unstemmed | In vitro antioxidant properties and antimicrobial activity of the ethanolic extract of Senecio nutans Sch. Beep. (Asteraceae) |
| title_short | In vitro antioxidant properties and antimicrobial activity of the ethanolic extract of Senecio nutans Sch. Beep. (Asteraceae) |
| title_sort | in vitro antioxidant properties and antimicrobial activity of the ethanolic extract of senecio nutans sch beep asteraceae |
| topic | antimicrobial activity chachacoma in vitro antioxidant activity phenolic compounds senecio nutans |
| url | https://jppres.com/jppres/pdf/vol10/jppres22.1471_10.6.1026.pdf |
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