Evaluation of the Potential of Lactobacillus paracasei Adjuncts for Flavor Compounds Development and Diversification in Short-Aged Cheddar Cheese
The non-starter microbiota of Cheddar cheese mostly comprises mesophilic lactobacilli, such as Lactobacillus casei, Lactobacillus paracasei, Lactobacillus rhamnosus, and Lactobacillus plantarum. These bacteria are recognized for their potential to improve Cheddar cheese flavor when used as adjunct c...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-07-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.01506/full |
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| author | Ewelina Stefanovic Kieran N. Kilcawley Clara Roces Mary C. Rea Mary C. Rea Maurice O'Sullivan Jeremiah J. Sheehan Olivia McAuliffe |
| author_facet | Ewelina Stefanovic Kieran N. Kilcawley Clara Roces Mary C. Rea Mary C. Rea Maurice O'Sullivan Jeremiah J. Sheehan Olivia McAuliffe |
| author_sort | Ewelina Stefanovic |
| collection | DOAJ |
| description | The non-starter microbiota of Cheddar cheese mostly comprises mesophilic lactobacilli, such as Lactobacillus casei, Lactobacillus paracasei, Lactobacillus rhamnosus, and Lactobacillus plantarum. These bacteria are recognized for their potential to improve Cheddar cheese flavor when used as adjunct cultures. In this study, three strains of L. paracasei (DPC2071, DPC4206, and DPC4536) were evaluated for their contribution to the enhancement and diversification of flavor in short-aged Cheddar cheese. The strains were selected based on their previously determined genomic diversity, variability in proteolytic enzyme activities and metabolic capability in cheese model systems. The addition of adjunct cultures did not affect the gross composition or levels of lipolysis of the cheeses. The levels of free amino acids (FAA) in cheeses showed a significant increase after 28 days of ripening. However, the concentrations of individual amino acids in the cheeses did not significantly differ except for some amino acids (aspartic acid, threonine, serine, and tryptophan) at Day 14. Volatile profile analysis revealed that the main compounds that differentiated the cheeses were of lipid origin, such as long chain aldehydes, acids, ketones, and lactones. This study demonstrated that the adjunct L. paracasei strains contributed to the development and diversification of compounds related to flavor in short-aged Cheddar cheeses. |
| first_indexed | 2024-04-12T22:27:50Z |
| format | Article |
| id | doaj.art-f0060678d4b446ebbbbd30def97933f8 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T22:27:50Z |
| publishDate | 2018-07-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-f0060678d4b446ebbbbd30def97933f82022-12-22T03:14:06ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-07-01910.3389/fmicb.2018.01506378167Evaluation of the Potential of Lactobacillus paracasei Adjuncts for Flavor Compounds Development and Diversification in Short-Aged Cheddar CheeseEwelina Stefanovic0Kieran N. Kilcawley1Clara Roces2Mary C. Rea3Mary C. Rea4Maurice O'Sullivan5Jeremiah J. Sheehan6Olivia McAuliffe7Department of Food Biosciences, Teagasc Food Research Centre, Fermoy, IrelandDepartment of Food Biosciences, Teagasc Food Research Centre, Fermoy, IrelandDepartment of Food Biosciences, Teagasc Food Research Centre, Fermoy, IrelandDepartment of Food Biosciences, Teagasc Food Research Centre, Fermoy, IrelandAPC Microbiome Ireland, University College Cork, Cork, IrelandSchool of Food and Nutritional Sciences, University College Cork, Cork, IrelandDepartment of Food Biosciences, Teagasc Food Research Centre, Fermoy, IrelandDepartment of Food Biosciences, Teagasc Food Research Centre, Fermoy, IrelandThe non-starter microbiota of Cheddar cheese mostly comprises mesophilic lactobacilli, such as Lactobacillus casei, Lactobacillus paracasei, Lactobacillus rhamnosus, and Lactobacillus plantarum. These bacteria are recognized for their potential to improve Cheddar cheese flavor when used as adjunct cultures. In this study, three strains of L. paracasei (DPC2071, DPC4206, and DPC4536) were evaluated for their contribution to the enhancement and diversification of flavor in short-aged Cheddar cheese. The strains were selected based on their previously determined genomic diversity, variability in proteolytic enzyme activities and metabolic capability in cheese model systems. The addition of adjunct cultures did not affect the gross composition or levels of lipolysis of the cheeses. The levels of free amino acids (FAA) in cheeses showed a significant increase after 28 days of ripening. However, the concentrations of individual amino acids in the cheeses did not significantly differ except for some amino acids (aspartic acid, threonine, serine, and tryptophan) at Day 14. Volatile profile analysis revealed that the main compounds that differentiated the cheeses were of lipid origin, such as long chain aldehydes, acids, ketones, and lactones. This study demonstrated that the adjunct L. paracasei strains contributed to the development and diversification of compounds related to flavor in short-aged Cheddar cheeses.https://www.frontiersin.org/article/10.3389/fmicb.2018.01506/fullLactobacillus paracaseidairycheese flavordiversificationvolatiles |
| spellingShingle | Ewelina Stefanovic Kieran N. Kilcawley Clara Roces Mary C. Rea Mary C. Rea Maurice O'Sullivan Jeremiah J. Sheehan Olivia McAuliffe Evaluation of the Potential of Lactobacillus paracasei Adjuncts for Flavor Compounds Development and Diversification in Short-Aged Cheddar Cheese Frontiers in Microbiology Lactobacillus paracasei dairy cheese flavor diversification volatiles |
| title | Evaluation of the Potential of Lactobacillus paracasei Adjuncts for Flavor Compounds Development and Diversification in Short-Aged Cheddar Cheese |
| title_full | Evaluation of the Potential of Lactobacillus paracasei Adjuncts for Flavor Compounds Development and Diversification in Short-Aged Cheddar Cheese |
| title_fullStr | Evaluation of the Potential of Lactobacillus paracasei Adjuncts for Flavor Compounds Development and Diversification in Short-Aged Cheddar Cheese |
| title_full_unstemmed | Evaluation of the Potential of Lactobacillus paracasei Adjuncts for Flavor Compounds Development and Diversification in Short-Aged Cheddar Cheese |
| title_short | Evaluation of the Potential of Lactobacillus paracasei Adjuncts for Flavor Compounds Development and Diversification in Short-Aged Cheddar Cheese |
| title_sort | evaluation of the potential of lactobacillus paracasei adjuncts for flavor compounds development and diversification in short aged cheddar cheese |
| topic | Lactobacillus paracasei dairy cheese flavor diversification volatiles |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.01506/full |
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