Minute Cellular Nodules as Early Lesions in Rats with Silica Exposure via Inhalation
Mechanisms of silicosis have yet to be clarified, and pathological conditions are inaccurately described in some experimental studies on silicosis. This study was aimed at describing initial lesions in silicosis, as observed in rats with silica exposure via inhalation, and major histopathologic alte...
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MDPI AG
2022-05-01
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| Series: | Veterinary Sciences |
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| Online Access: | https://www.mdpi.com/2306-7381/9/6/251 |
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| author | Yaqian Li Fuyu Jin Tian Li Xinyu Yang Wenchen Cai Shifeng Li Xuemin Gao Na Mao Heliang Liu Hong Xu Fang Yang |
| author_facet | Yaqian Li Fuyu Jin Tian Li Xinyu Yang Wenchen Cai Shifeng Li Xuemin Gao Na Mao Heliang Liu Hong Xu Fang Yang |
| author_sort | Yaqian Li |
| collection | DOAJ |
| description | Mechanisms of silicosis have yet to be clarified, and pathological conditions are inaccurately described in some experimental studies on silicosis. This study was aimed at describing initial lesions in silicosis, as observed in rats with silica exposure via inhalation, and major histopathologic alterations. Male Wistar rats were exposed to silica for 24 weeks. Hematoxylin and eosin staining indicated the presence of “cellular nodule+ macrophage alveolitis” in rats exposed to silica from the 2–16 weeks time points and “fibrotic cellular + cellular nodule” in rats exposed to silica via inhalation for 24 weeks. By immunohistochemistry, the following were noted: a continual increase in the positive expression of CD68 in macrophages in the lungs of rats exposed to silica; hyperplasia in alveolar type II cells (AT2); loss of original phenotypes in fibrotic cellular nodules, macrophages, and AT2 cells; loss of endothelial cells in silicotic nodules; and positive expression of α-smooth muscle actin in macrophages. Typical pathological changes in silicosis were also summarized. Among these changes were macrophage alveolitis, cellular nodules, and fibrotic cellular nodules, including an increase in minute cellular nodules in the early stages and the formation of fibrotic cellular nodules in the late stages. |
| first_indexed | 2024-03-09T22:16:46Z |
| format | Article |
| id | doaj.art-f006990dd10c410dbc89b3965a02164e |
| institution | Directory Open Access Journal |
| issn | 2306-7381 |
| language | English |
| last_indexed | 2024-03-09T22:16:46Z |
| publishDate | 2022-05-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Veterinary Sciences |
| spelling | doaj.art-f006990dd10c410dbc89b3965a02164e2023-11-23T19:22:48ZengMDPI AGVeterinary Sciences2306-73812022-05-019625110.3390/vetsci9060251Minute Cellular Nodules as Early Lesions in Rats with Silica Exposure via InhalationYaqian Li0Fuyu Jin1Tian Li2Xinyu Yang3Wenchen Cai4Shifeng Li5Xuemin Gao6Na Mao7Heliang Liu8Hong Xu9Fang Yang10Hebei Key Laboratory for Organ Fibrosis Research, School of Public Health, North China University of Science and Technology, Tangshan 063210, ChinaHebei Key Laboratory for Organ Fibrosis Research, School of Public Health, North China University of Science and Technology, Tangshan 063210, ChinaHebei Key Laboratory for Organ Fibrosis Research, School of Public Health, North China University of Science and Technology, Tangshan 063210, ChinaHebei Key Laboratory for Organ Fibrosis Research, School of Public Health, North China University of Science and Technology, Tangshan 063210, ChinaHebei Key Laboratory for Organ Fibrosis Research, School of Public Health, North China University of Science and Technology, Tangshan 063210, ChinaHebei Key Laboratory for Organ Fibrosis Research, School of Public Health, North China University of Science and Technology, Tangshan 063210, ChinaHebei Key Laboratory for Organ Fibrosis Research, School of Public Health, North China University of Science and Technology, Tangshan 063210, ChinaHebei Key Laboratory for Organ Fibrosis Research, School of Public Health, North China University of Science and Technology, Tangshan 063210, ChinaHebei Key Laboratory for Organ Fibrosis Research, School of Public Health, North China University of Science and Technology, Tangshan 063210, ChinaHebei Key Laboratory for Organ Fibrosis Research, School of Public Health, North China University of Science and Technology, Tangshan 063210, ChinaHebei Key Laboratory for Organ Fibrosis Research, School of Public Health, North China University of Science and Technology, Tangshan 063210, ChinaMechanisms of silicosis have yet to be clarified, and pathological conditions are inaccurately described in some experimental studies on silicosis. This study was aimed at describing initial lesions in silicosis, as observed in rats with silica exposure via inhalation, and major histopathologic alterations. Male Wistar rats were exposed to silica for 24 weeks. Hematoxylin and eosin staining indicated the presence of “cellular nodule+ macrophage alveolitis” in rats exposed to silica from the 2–16 weeks time points and “fibrotic cellular + cellular nodule” in rats exposed to silica via inhalation for 24 weeks. By immunohistochemistry, the following were noted: a continual increase in the positive expression of CD68 in macrophages in the lungs of rats exposed to silica; hyperplasia in alveolar type II cells (AT2); loss of original phenotypes in fibrotic cellular nodules, macrophages, and AT2 cells; loss of endothelial cells in silicotic nodules; and positive expression of α-smooth muscle actin in macrophages. Typical pathological changes in silicosis were also summarized. Among these changes were macrophage alveolitis, cellular nodules, and fibrotic cellular nodules, including an increase in minute cellular nodules in the early stages and the formation of fibrotic cellular nodules in the late stages.https://www.mdpi.com/2306-7381/9/6/251ratssilicosismacrophage alveolitiscellular nodulesfibrotic cellular nodules |
| spellingShingle | Yaqian Li Fuyu Jin Tian Li Xinyu Yang Wenchen Cai Shifeng Li Xuemin Gao Na Mao Heliang Liu Hong Xu Fang Yang Minute Cellular Nodules as Early Lesions in Rats with Silica Exposure via Inhalation Veterinary Sciences rats silicosis macrophage alveolitis cellular nodules fibrotic cellular nodules |
| title | Minute Cellular Nodules as Early Lesions in Rats with Silica Exposure via Inhalation |
| title_full | Minute Cellular Nodules as Early Lesions in Rats with Silica Exposure via Inhalation |
| title_fullStr | Minute Cellular Nodules as Early Lesions in Rats with Silica Exposure via Inhalation |
| title_full_unstemmed | Minute Cellular Nodules as Early Lesions in Rats with Silica Exposure via Inhalation |
| title_short | Minute Cellular Nodules as Early Lesions in Rats with Silica Exposure via Inhalation |
| title_sort | minute cellular nodules as early lesions in rats with silica exposure via inhalation |
| topic | rats silicosis macrophage alveolitis cellular nodules fibrotic cellular nodules |
| url | https://www.mdpi.com/2306-7381/9/6/251 |
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