Seroconversion in COVID-19 Infection and Comparison of Antibody Responses in Symptomatic Versus Asymptomatic Individuals
Coronavirus disease (COVID-19) is an infectious disease caused by a newly discovered coronavirus. Following infection, antibodies are formed against the spike (S) and nucleocapsid (N) proteins, which are the primary viral antigens of SARS-CoV-2. This study aims to determine the antibody response thr...
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| Format: | Article |
| Language: | English |
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Journal of Pure and Applied Microbiology
2023-03-01
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| Series: | Journal of Pure and Applied Microbiology |
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| Online Access: | https://microbiologyjournal.org/seroconversion-in-covid-19-infection-and-comparison-of-antibody-responses-in-symptomatic-versus-asymptomatic-individuals/ |
| _version_ | 1797867147607670784 |
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| author | S. Thasneem Banu S. Vinotha Radhika Katragadda R. Vanaja |
| author_facet | S. Thasneem Banu S. Vinotha Radhika Katragadda R. Vanaja |
| author_sort | S. Thasneem Banu |
| collection | DOAJ |
| description | Coronavirus disease (COVID-19) is an infectious disease caused by a newly discovered coronavirus. Following infection, antibodies are formed against the spike (S) and nucleocapsid (N) proteins, which are the primary viral antigens of SARS-CoV-2. This study aims to determine the antibody response three weeks post-infection and its persistence. To study antibody responses in COVID-19-positive individuals and to compare the degree of antibody response in symptomatic and asymptomatic individuals. The persistence of the antibody response was also assessed. Adult patients (> 15 years of age) who were diagnosed as COVID-19-positive by RT-PCR, three weeks after swab positivity were tested for total antibody levels against COVID-19 antigens by electrochemiluminescence assay. Out of 226 individuals, 129 were symptomatic and 97 were asymptomatic. Among the 129 symptomatic individuals, 74 exhibited an antibody response, whereas in the asymptomatic individuals, only 10 exhibited an antibody response. The antibody response was found to be significant in symptomatic individuals compared to that in asymptomatic individuals (p < 0.05). All follow-up individuals were seropositive at the end of both 6 and 8 months. Antibodies against SARS-CoV-2 persist for 8 months following infection. Despite the waning of antibodies against the nucleocapsid antigen, there was no complete disappearance of antibodies. |
| first_indexed | 2024-04-09T23:35:41Z |
| format | Article |
| id | doaj.art-f006a307e76d41918e0a554ef6689039 |
| institution | Directory Open Access Journal |
| issn | 0973-7510 2581-690X |
| language | English |
| last_indexed | 2024-04-09T23:35:41Z |
| publishDate | 2023-03-01 |
| publisher | Journal of Pure and Applied Microbiology |
| record_format | Article |
| series | Journal of Pure and Applied Microbiology |
| spelling | doaj.art-f006a307e76d41918e0a554ef66890392023-03-20T12:14:22ZengJournal of Pure and Applied MicrobiologyJournal of Pure and Applied Microbiology0973-75102581-690X2023-03-01171590596https://doi.org/10.22207/JPAM.17.1.56Seroconversion in COVID-19 Infection and Comparison of Antibody Responses in Symptomatic Versus Asymptomatic IndividualsS. Thasneem Banuhttps://orcid.org/0000-0001-7810-8183 S. Vinothahttps://orcid.org/0000-0003-1625-8869Radhika Katragaddahttps://orcid.org/0000-0002-6756-7306R. Vanajahttps://orcid.org/0009-0001-2465-3917Coronavirus disease (COVID-19) is an infectious disease caused by a newly discovered coronavirus. Following infection, antibodies are formed against the spike (S) and nucleocapsid (N) proteins, which are the primary viral antigens of SARS-CoV-2. This study aims to determine the antibody response three weeks post-infection and its persistence. To study antibody responses in COVID-19-positive individuals and to compare the degree of antibody response in symptomatic and asymptomatic individuals. The persistence of the antibody response was also assessed. Adult patients (> 15 years of age) who were diagnosed as COVID-19-positive by RT-PCR, three weeks after swab positivity were tested for total antibody levels against COVID-19 antigens by electrochemiluminescence assay. Out of 226 individuals, 129 were symptomatic and 97 were asymptomatic. Among the 129 symptomatic individuals, 74 exhibited an antibody response, whereas in the asymptomatic individuals, only 10 exhibited an antibody response. The antibody response was found to be significant in symptomatic individuals compared to that in asymptomatic individuals (p < 0.05). All follow-up individuals were seropositive at the end of both 6 and 8 months. Antibodies against SARS-CoV-2 persist for 8 months following infection. Despite the waning of antibodies against the nucleocapsid antigen, there was no complete disappearance of antibodies.https://microbiologyjournal.org/seroconversion-in-covid-19-infection-and-comparison-of-antibody-responses-in-symptomatic-versus-asymptomatic-individuals/covid-19rt-pcrelectrochemiluminescence assay |
| spellingShingle | S. Thasneem Banu S. Vinotha Radhika Katragadda R. Vanaja Seroconversion in COVID-19 Infection and Comparison of Antibody Responses in Symptomatic Versus Asymptomatic Individuals Journal of Pure and Applied Microbiology covid-19 rt-pcr electrochemiluminescence assay |
| title | Seroconversion in COVID-19 Infection and Comparison of Antibody Responses in Symptomatic Versus Asymptomatic Individuals |
| title_full | Seroconversion in COVID-19 Infection and Comparison of Antibody Responses in Symptomatic Versus Asymptomatic Individuals |
| title_fullStr | Seroconversion in COVID-19 Infection and Comparison of Antibody Responses in Symptomatic Versus Asymptomatic Individuals |
| title_full_unstemmed | Seroconversion in COVID-19 Infection and Comparison of Antibody Responses in Symptomatic Versus Asymptomatic Individuals |
| title_short | Seroconversion in COVID-19 Infection and Comparison of Antibody Responses in Symptomatic Versus Asymptomatic Individuals |
| title_sort | seroconversion in covid 19 infection and comparison of antibody responses in symptomatic versus asymptomatic individuals |
| topic | covid-19 rt-pcr electrochemiluminescence assay |
| url | https://microbiologyjournal.org/seroconversion-in-covid-19-infection-and-comparison-of-antibody-responses-in-symptomatic-versus-asymptomatic-individuals/ |
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