Using Mesoporous Silica-Based Dual Biomimetic Nano-Erythrocytes for an Improved Antitumor Effect
The nano-delivery system with a dual biomimetic effect can penetrate deeper in tumor microenvironments (TMEs) and release sufficient antitumor drugs, which has attracted much attention. In this study, we synthesized erythrocyte-like mesoporous silica nanoparticles (EMSNs) as the core loaded with dox...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-12-01
|
| Series: | Pharmaceutics |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1999-4923/15/12/2785 |
| _version_ | 1797379680922238976 |
|---|---|
| author | Ziyue Xi Yingying Jiang Zitong Ma Qun Li Xinran Xi Chuanyong Fan Shuang Zhu Junjie Zhang Lu Xu |
| author_facet | Ziyue Xi Yingying Jiang Zitong Ma Qun Li Xinran Xi Chuanyong Fan Shuang Zhu Junjie Zhang Lu Xu |
| author_sort | Ziyue Xi |
| collection | DOAJ |
| description | The nano-delivery system with a dual biomimetic effect can penetrate deeper in tumor microenvironments (TMEs) and release sufficient antitumor drugs, which has attracted much attention. In this study, we synthesized erythrocyte-like mesoporous silica nanoparticles (EMSNs) as the core loaded with doxorubicin (DOX) and coated them with calcium phosphate (CaP) and erythrocyte membrane (EM) to obtain DOX/EsPMs. The transmission electron microscopy (TEM), fluorescent co-localization and protein bands of SDS-PAGE were used to confirm the complete fabrication of EsPMs. The EsPMs with erythrocyte-like shape exhibited superior penetration ability in in vitro diffusion and tumor-sphere penetration experiments. Intracellular Ca<sup>2+</sup> and ROS detection experiments showed that the CaP membranes of EsPMs with pH-sensitivity could provide Ca<sup>2+</sup> continuously to induce reactive oxide species’ (ROS) generation in the TME. The EM as a perfect “camouflaged clothing” which could confuse macrophagocytes into prolonging blood circulation. Hemolysis and non-specific protein adsorption tests proved the desirable biocompatibility of EsPMs. An in vivo pharmacodynamics evaluation showed that the DOX/EsPMs group had a satisfactory tumor-inhibition effect. These advantages of the nano-erythrocytes suggest that by modifying the existing materials to construct a nano-delivery system, nanoparticles will achieve a biomimetic effect from both their structure and function with a facilitated and sufficient drug release profile, which is of great significance for antitumor therapy. |
| first_indexed | 2024-03-08T20:26:30Z |
| format | Article |
| id | doaj.art-f00b0560dfc047aea44abdc036a2ec72 |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-08T20:26:30Z |
| publishDate | 2023-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-f00b0560dfc047aea44abdc036a2ec722023-12-22T14:32:20ZengMDPI AGPharmaceutics1999-49232023-12-011512278510.3390/pharmaceutics15122785Using Mesoporous Silica-Based Dual Biomimetic Nano-Erythrocytes for an Improved Antitumor EffectZiyue Xi0Yingying Jiang1Zitong Ma2Qun Li3Xinran Xi4Chuanyong Fan5Shuang Zhu6Junjie Zhang7Lu Xu8School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, ChinaSchool of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, ChinaSchool of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, ChinaSchool of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, ChinaSchool of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, ChinaSchool of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, ChinaSchool of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, ChinaSchool of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, ChinaSchool of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, ChinaThe nano-delivery system with a dual biomimetic effect can penetrate deeper in tumor microenvironments (TMEs) and release sufficient antitumor drugs, which has attracted much attention. In this study, we synthesized erythrocyte-like mesoporous silica nanoparticles (EMSNs) as the core loaded with doxorubicin (DOX) and coated them with calcium phosphate (CaP) and erythrocyte membrane (EM) to obtain DOX/EsPMs. The transmission electron microscopy (TEM), fluorescent co-localization and protein bands of SDS-PAGE were used to confirm the complete fabrication of EsPMs. The EsPMs with erythrocyte-like shape exhibited superior penetration ability in in vitro diffusion and tumor-sphere penetration experiments. Intracellular Ca<sup>2+</sup> and ROS detection experiments showed that the CaP membranes of EsPMs with pH-sensitivity could provide Ca<sup>2+</sup> continuously to induce reactive oxide species’ (ROS) generation in the TME. The EM as a perfect “camouflaged clothing” which could confuse macrophagocytes into prolonging blood circulation. Hemolysis and non-specific protein adsorption tests proved the desirable biocompatibility of EsPMs. An in vivo pharmacodynamics evaluation showed that the DOX/EsPMs group had a satisfactory tumor-inhibition effect. These advantages of the nano-erythrocytes suggest that by modifying the existing materials to construct a nano-delivery system, nanoparticles will achieve a biomimetic effect from both their structure and function with a facilitated and sufficient drug release profile, which is of great significance for antitumor therapy.https://www.mdpi.com/1999-4923/15/12/2785biomimeticerythrocyte-like nanoparticleserythrocyte membranepH-sensitivityantitumor |
| spellingShingle | Ziyue Xi Yingying Jiang Zitong Ma Qun Li Xinran Xi Chuanyong Fan Shuang Zhu Junjie Zhang Lu Xu Using Mesoporous Silica-Based Dual Biomimetic Nano-Erythrocytes for an Improved Antitumor Effect Pharmaceutics biomimetic erythrocyte-like nanoparticles erythrocyte membrane pH-sensitivity antitumor |
| title | Using Mesoporous Silica-Based Dual Biomimetic Nano-Erythrocytes for an Improved Antitumor Effect |
| title_full | Using Mesoporous Silica-Based Dual Biomimetic Nano-Erythrocytes for an Improved Antitumor Effect |
| title_fullStr | Using Mesoporous Silica-Based Dual Biomimetic Nano-Erythrocytes for an Improved Antitumor Effect |
| title_full_unstemmed | Using Mesoporous Silica-Based Dual Biomimetic Nano-Erythrocytes for an Improved Antitumor Effect |
| title_short | Using Mesoporous Silica-Based Dual Biomimetic Nano-Erythrocytes for an Improved Antitumor Effect |
| title_sort | using mesoporous silica based dual biomimetic nano erythrocytes for an improved antitumor effect |
| topic | biomimetic erythrocyte-like nanoparticles erythrocyte membrane pH-sensitivity antitumor |
| url | https://www.mdpi.com/1999-4923/15/12/2785 |
| work_keys_str_mv | AT ziyuexi usingmesoporoussilicabaseddualbiomimeticnanoerythrocytesforanimprovedantitumoreffect AT yingyingjiang usingmesoporoussilicabaseddualbiomimeticnanoerythrocytesforanimprovedantitumoreffect AT zitongma usingmesoporoussilicabaseddualbiomimeticnanoerythrocytesforanimprovedantitumoreffect AT qunli usingmesoporoussilicabaseddualbiomimeticnanoerythrocytesforanimprovedantitumoreffect AT xinranxi usingmesoporoussilicabaseddualbiomimeticnanoerythrocytesforanimprovedantitumoreffect AT chuanyongfan usingmesoporoussilicabaseddualbiomimeticnanoerythrocytesforanimprovedantitumoreffect AT shuangzhu usingmesoporoussilicabaseddualbiomimeticnanoerythrocytesforanimprovedantitumoreffect AT junjiezhang usingmesoporoussilicabaseddualbiomimeticnanoerythrocytesforanimprovedantitumoreffect AT luxu usingmesoporoussilicabaseddualbiomimeticnanoerythrocytesforanimprovedantitumoreffect |