On Fusogenicity of Positively Charged Phased-Separated Lipid Vesicles: Experiments and Computational Simulations
This paper studies the fusogenicity of cationic liposomes in relation to their surface distribution of cationic lipids and utilizes membrane phase separation to control this surface distribution. It is found that concentrating the cationic lipids into small surface patches on liposomes, through phas...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/13/10/1473 |
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| author | Yifei Wang Yerbol Palzhanov Dang T. Dang Annalisa Quaini Maxim Olshanskii Sheereen Majd |
| author_facet | Yifei Wang Yerbol Palzhanov Dang T. Dang Annalisa Quaini Maxim Olshanskii Sheereen Majd |
| author_sort | Yifei Wang |
| collection | DOAJ |
| description | This paper studies the fusogenicity of cationic liposomes in relation to their surface distribution of cationic lipids and utilizes membrane phase separation to control this surface distribution. It is found that concentrating the cationic lipids into small surface patches on liposomes, through phase-separation, can enhance liposome’s fusogenicity. Further concentrating these lipids into smaller patches on the surface of liposomes led to an increased level of fusogenicity. These experimental findings are supported by numerical simulations using a mathematical model for phase-separated charged liposomes. Findings of this study may be used for design and development of highly fusogenic liposomes with minimal level of toxicity. |
| first_indexed | 2024-03-10T21:24:59Z |
| format | Article |
| id | doaj.art-f00c657ada8f4915b5ecc3b3077830b4 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T21:24:59Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-f00c657ada8f4915b5ecc3b3077830b42023-11-19T15:49:45ZengMDPI AGBiomolecules2218-273X2023-09-011310147310.3390/biom13101473On Fusogenicity of Positively Charged Phased-Separated Lipid Vesicles: Experiments and Computational SimulationsYifei Wang0Yerbol Palzhanov1Dang T. Dang2Annalisa Quaini3Maxim Olshanskii4Sheereen Majd5Department of Biomedical Engineering, University of Houston, 3551 Cullen Blvd, Houston, TX 77204, USADepartment of Mathematics, University of Houston, 3551 Cullen Blvd, Houston, TX 77204, USADepartment of Biomedical Engineering, University of Houston, 3551 Cullen Blvd, Houston, TX 77204, USADepartment of Mathematics, University of Houston, 3551 Cullen Blvd, Houston, TX 77204, USADepartment of Mathematics, University of Houston, 3551 Cullen Blvd, Houston, TX 77204, USADepartment of Biomedical Engineering, University of Houston, 3551 Cullen Blvd, Houston, TX 77204, USAThis paper studies the fusogenicity of cationic liposomes in relation to their surface distribution of cationic lipids and utilizes membrane phase separation to control this surface distribution. It is found that concentrating the cationic lipids into small surface patches on liposomes, through phase-separation, can enhance liposome’s fusogenicity. Further concentrating these lipids into smaller patches on the surface of liposomes led to an increased level of fusogenicity. These experimental findings are supported by numerical simulations using a mathematical model for phase-separated charged liposomes. Findings of this study may be used for design and development of highly fusogenic liposomes with minimal level of toxicity.https://www.mdpi.com/2218-273X/13/10/1473membrane phase separationfusogenic liposomescationic lipidsfluorescence microscopycomputational modeling |
| spellingShingle | Yifei Wang Yerbol Palzhanov Dang T. Dang Annalisa Quaini Maxim Olshanskii Sheereen Majd On Fusogenicity of Positively Charged Phased-Separated Lipid Vesicles: Experiments and Computational Simulations Biomolecules membrane phase separation fusogenic liposomes cationic lipids fluorescence microscopy computational modeling |
| title | On Fusogenicity of Positively Charged Phased-Separated Lipid Vesicles: Experiments and Computational Simulations |
| title_full | On Fusogenicity of Positively Charged Phased-Separated Lipid Vesicles: Experiments and Computational Simulations |
| title_fullStr | On Fusogenicity of Positively Charged Phased-Separated Lipid Vesicles: Experiments and Computational Simulations |
| title_full_unstemmed | On Fusogenicity of Positively Charged Phased-Separated Lipid Vesicles: Experiments and Computational Simulations |
| title_short | On Fusogenicity of Positively Charged Phased-Separated Lipid Vesicles: Experiments and Computational Simulations |
| title_sort | on fusogenicity of positively charged phased separated lipid vesicles experiments and computational simulations |
| topic | membrane phase separation fusogenic liposomes cationic lipids fluorescence microscopy computational modeling |
| url | https://www.mdpi.com/2218-273X/13/10/1473 |
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