Molecular occurrence and genetic diversity of Ehrlichia canis in naturally infected dogs from Thailand
Abstract Canine monocytic ehrlichiosis is cause by Ehrlichia canis resulting in hematologic disorders and severe clinical signs. The aim of this study was to scrutinize the molecular detection and genetic diversity of E. canis based on the trp36 gene in dogs from Thailand’s northern and central regi...
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| Format: | Article |
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Nature Portfolio
2023-11-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-023-47784-4 |
| _version_ | 1797453033641082880 |
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| author | Napassorn Poolsawat Siriphan Sangchuai Tassanee Jaroensak Amaya Watthanadirek-Wijidwong Nitipon Srionrod Sutthida Minsakorn Keiichiro Tazawa Panat Anuracpreeda |
| author_facet | Napassorn Poolsawat Siriphan Sangchuai Tassanee Jaroensak Amaya Watthanadirek-Wijidwong Nitipon Srionrod Sutthida Minsakorn Keiichiro Tazawa Panat Anuracpreeda |
| author_sort | Napassorn Poolsawat |
| collection | DOAJ |
| description | Abstract Canine monocytic ehrlichiosis is cause by Ehrlichia canis resulting in hematologic disorders and severe clinical signs. The aim of this study was to scrutinize the molecular detection and genetic diversity of E. canis based on the trp36 gene in dogs from Thailand’s northern and central regions. A total of 120 dogs blood samples were amplified for trp36 gene of E. canis using the polymerase chain reaction (PCR). Forty-seven out of 120 dog blood samples (39.16%, 47/120) were positive for E. canis the trp36 DNA with 790 bp of PCR amplicon size. The factor significantly associated with E. canis infection is animal housing status (p < 0.05). Sequence and phylogenetic analysis showed that E. canis trp36 gene of Thailand isolates was clustered into 1st clade with similarity ranging from 95.65 to 100% together with the US genogroup. The 14 haplotypes of the trp36 gene shown in TCS network exhibited that haplotype #1–4 was found in Thailand. The entropy analysis of the trp36 gene illustrated 751 polymorphic sites and 271 entropy peaks of nucleic and amino acid sequences, respectively. Hence, these findings are crucial for better understanding the epidemiology of Ehrlichia infection and could be helpful for implementing control measures in Thailand. |
| first_indexed | 2024-03-09T15:17:07Z |
| format | Article |
| id | doaj.art-f00e8b0ff0d64775aa31520f18cde16f |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-09T15:17:07Z |
| publishDate | 2023-11-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-f00e8b0ff0d64775aa31520f18cde16f2023-11-26T13:02:21ZengNature PortfolioScientific Reports2045-23222023-11-0113111110.1038/s41598-023-47784-4Molecular occurrence and genetic diversity of Ehrlichia canis in naturally infected dogs from ThailandNapassorn Poolsawat0Siriphan Sangchuai1Tassanee Jaroensak2Amaya Watthanadirek-Wijidwong3Nitipon Srionrod4Sutthida Minsakorn5Keiichiro Tazawa6Panat Anuracpreeda7Parasitology Research Laboratory (PRL), Institute of Molecular Biosciences, Mahidol UniversityParasitology Research Laboratory (PRL), Institute of Molecular Biosciences, Mahidol UniversityParasitology Research Laboratory (PRL), Institute of Molecular Biosciences, Mahidol UniversityParasitology Research Laboratory (PRL), Institute of Molecular Biosciences, Mahidol UniversityParasitology Research Laboratory (PRL), Institute of Molecular Biosciences, Mahidol UniversityParasitology Research Laboratory (PRL), Institute of Molecular Biosciences, Mahidol UniversityWorldwide Veterinary Service ThailandParasitology Research Laboratory (PRL), Institute of Molecular Biosciences, Mahidol UniversityAbstract Canine monocytic ehrlichiosis is cause by Ehrlichia canis resulting in hematologic disorders and severe clinical signs. The aim of this study was to scrutinize the molecular detection and genetic diversity of E. canis based on the trp36 gene in dogs from Thailand’s northern and central regions. A total of 120 dogs blood samples were amplified for trp36 gene of E. canis using the polymerase chain reaction (PCR). Forty-seven out of 120 dog blood samples (39.16%, 47/120) were positive for E. canis the trp36 DNA with 790 bp of PCR amplicon size. The factor significantly associated with E. canis infection is animal housing status (p < 0.05). Sequence and phylogenetic analysis showed that E. canis trp36 gene of Thailand isolates was clustered into 1st clade with similarity ranging from 95.65 to 100% together with the US genogroup. The 14 haplotypes of the trp36 gene shown in TCS network exhibited that haplotype #1–4 was found in Thailand. The entropy analysis of the trp36 gene illustrated 751 polymorphic sites and 271 entropy peaks of nucleic and amino acid sequences, respectively. Hence, these findings are crucial for better understanding the epidemiology of Ehrlichia infection and could be helpful for implementing control measures in Thailand.https://doi.org/10.1038/s41598-023-47784-4 |
| spellingShingle | Napassorn Poolsawat Siriphan Sangchuai Tassanee Jaroensak Amaya Watthanadirek-Wijidwong Nitipon Srionrod Sutthida Minsakorn Keiichiro Tazawa Panat Anuracpreeda Molecular occurrence and genetic diversity of Ehrlichia canis in naturally infected dogs from Thailand Scientific Reports |
| title | Molecular occurrence and genetic diversity of Ehrlichia canis in naturally infected dogs from Thailand |
| title_full | Molecular occurrence and genetic diversity of Ehrlichia canis in naturally infected dogs from Thailand |
| title_fullStr | Molecular occurrence and genetic diversity of Ehrlichia canis in naturally infected dogs from Thailand |
| title_full_unstemmed | Molecular occurrence and genetic diversity of Ehrlichia canis in naturally infected dogs from Thailand |
| title_short | Molecular occurrence and genetic diversity of Ehrlichia canis in naturally infected dogs from Thailand |
| title_sort | molecular occurrence and genetic diversity of ehrlichia canis in naturally infected dogs from thailand |
| url | https://doi.org/10.1038/s41598-023-47784-4 |
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