Comparative Transcriptomic Analysis of Subcutaneous Adipose Tissue from Local Pig Breeds
When compared to modern lean-type breeds, Portuguese local Alentejano (AL) and Bísaro (BI) pig breeds present a high potential for subcutaneous and intramuscular fat (IMF) deposition which contributes for better meat quality. The aim of this work was to explore the genome function to better understa...
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MDPI AG
2020-04-01
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| Series: | Genes |
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| Online Access: | https://www.mdpi.com/2073-4425/11/4/422 |
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| author | André Albuquerque Cristina Óvilo Yolanda Núñez Rita Benítez Adrián López-Garcia Fabián García Maria do Rosário Félix Marta Laranjo Rui Charneca José Manuel Martins |
| author_facet | André Albuquerque Cristina Óvilo Yolanda Núñez Rita Benítez Adrián López-Garcia Fabián García Maria do Rosário Félix Marta Laranjo Rui Charneca José Manuel Martins |
| author_sort | André Albuquerque |
| collection | DOAJ |
| description | When compared to modern lean-type breeds, Portuguese local Alentejano (AL) and Bísaro (BI) pig breeds present a high potential for subcutaneous and intramuscular fat (IMF) deposition which contributes for better meat quality. The aim of this work was to explore the genome function to better understand the underlying physiological mechanisms associated with body fat accretion. Dorsal subcutaneous fat samples were collected at slaughter from adult animals (<i>n</i> = 4 for each breed) with ~150 kg body weight. Total RNA was obtained and sequenced for transcriptome analysis using DESeq2. A total of 458 differentially expressed (DE) genes (q-value < 0.05) were identified, with 263 overexpressed in AL and 195 in BI. Key genes involved in <i>de novo</i> fatty acid biosynthesis, elongation and desaturation were upregulated in AL such as <i>ACLY</i>, <i>FASN</i>, <i>ME1</i>, <i>ELOVL6</i> and <i>SCD</i>. A functional enrichment analysis of the DE genes was performed using Ingenuity Pathway Analysis. Cholesterol synthesis is suggested to be higher in AL via SREBF2, SCAP and PPARG, while lipolytic activity may be more active in BI through GH and AMPK signalling. Increased signalling of CD40 together with the predicted activation of INSIG1 and INSIG2 in BI suggests that this breed is more sensitive to insulin whereas the AL is less sensitive like the Iberian breed. |
| first_indexed | 2024-03-10T20:28:36Z |
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| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-10T20:28:36Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-f00e9465c3ee49a4973209d472a5d9792023-11-19T21:38:41ZengMDPI AGGenes2073-44252020-04-0111442210.3390/genes11040422Comparative Transcriptomic Analysis of Subcutaneous Adipose Tissue from Local Pig BreedsAndré Albuquerque0Cristina Óvilo1Yolanda Núñez2Rita Benítez3Adrián López-Garcia4Fabián García5Maria do Rosário Félix6Marta Laranjo7Rui Charneca8José Manuel Martins9MED-Mediterranean Institute for Agriculture, Environment and Development, Instituto de Investigação e Formação Avançada & Universidade de Évora, Pólo da Mitra, Ap. 94, 7006-554 Évora, PortugalDepartamento de Mejora Genética Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 28040 Madrid, SpainDepartamento de Mejora Genética Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 28040 Madrid, SpainDepartamento de Mejora Genética Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 28040 Madrid, SpainDepartamento de Mejora Genética Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 28040 Madrid, SpainDepartamento de Mejora Genética Animal, Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), 28040 Madrid, SpainMED & Departamento de Fitotecnia, Escola de Ciências e Tecnologia, Universidade de Évora, Pólo da Mitra, Ap. 94, 7006-554 Évora, PortugalMED-Mediterranean Institute for Agriculture, Environment and Development, Instituto de Investigação e Formação Avançada & Universidade de Évora, Pólo da Mitra, Ap. 94, 7006-554 Évora, PortugalMED & Departamento de Medicina Veterinária, Escola de Ciências e Tecnologia, Universidade de Évora, Pólo da Mitra, Ap. 94, 7006-554 Évora, PortugalMED & Departamento de Zootecnia, Escola de Ciências e Tecnologia, Universidade de Évora, Pólo da Mitra, Ap. 94, 7006-554 Évora, PortugalWhen compared to modern lean-type breeds, Portuguese local Alentejano (AL) and Bísaro (BI) pig breeds present a high potential for subcutaneous and intramuscular fat (IMF) deposition which contributes for better meat quality. The aim of this work was to explore the genome function to better understand the underlying physiological mechanisms associated with body fat accretion. Dorsal subcutaneous fat samples were collected at slaughter from adult animals (<i>n</i> = 4 for each breed) with ~150 kg body weight. Total RNA was obtained and sequenced for transcriptome analysis using DESeq2. A total of 458 differentially expressed (DE) genes (q-value < 0.05) were identified, with 263 overexpressed in AL and 195 in BI. Key genes involved in <i>de novo</i> fatty acid biosynthesis, elongation and desaturation were upregulated in AL such as <i>ACLY</i>, <i>FASN</i>, <i>ME1</i>, <i>ELOVL6</i> and <i>SCD</i>. A functional enrichment analysis of the DE genes was performed using Ingenuity Pathway Analysis. Cholesterol synthesis is suggested to be higher in AL via SREBF2, SCAP and PPARG, while lipolytic activity may be more active in BI through GH and AMPK signalling. Increased signalling of CD40 together with the predicted activation of INSIG1 and INSIG2 in BI suggests that this breed is more sensitive to insulin whereas the AL is less sensitive like the Iberian breed.https://www.mdpi.com/2073-4425/11/4/422Alentejano pigBísaro pigRNA-seqdifferentially expressed genes (DEGs)Dorsal subcutaneous fattranscriptome |
| spellingShingle | André Albuquerque Cristina Óvilo Yolanda Núñez Rita Benítez Adrián López-Garcia Fabián García Maria do Rosário Félix Marta Laranjo Rui Charneca José Manuel Martins Comparative Transcriptomic Analysis of Subcutaneous Adipose Tissue from Local Pig Breeds Genes Alentejano pig Bísaro pig RNA-seq differentially expressed genes (DEGs) Dorsal subcutaneous fat transcriptome |
| title | Comparative Transcriptomic Analysis of Subcutaneous Adipose Tissue from Local Pig Breeds |
| title_full | Comparative Transcriptomic Analysis of Subcutaneous Adipose Tissue from Local Pig Breeds |
| title_fullStr | Comparative Transcriptomic Analysis of Subcutaneous Adipose Tissue from Local Pig Breeds |
| title_full_unstemmed | Comparative Transcriptomic Analysis of Subcutaneous Adipose Tissue from Local Pig Breeds |
| title_short | Comparative Transcriptomic Analysis of Subcutaneous Adipose Tissue from Local Pig Breeds |
| title_sort | comparative transcriptomic analysis of subcutaneous adipose tissue from local pig breeds |
| topic | Alentejano pig Bísaro pig RNA-seq differentially expressed genes (DEGs) Dorsal subcutaneous fat transcriptome |
| url | https://www.mdpi.com/2073-4425/11/4/422 |
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