Replication and virulence in pigs of the first African swine fever virus isolated in China
ABSTRACTAfrican swine fever (ASF) entered China in August 2018 and rapidly spread across the entire country, severely threatening the Chinese domestic pig population, which accounts for more than 50% of the pig population worldwide. In this study, an ASFV isolate, Pig/Heilongjiang/2018 (Pig/HLJ/18),...
| Main Authors: | , , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2019-01-01
|
| Series: | Emerging Microbes and Infections |
| Subjects: | |
| Online Access: | https://www.tandfonline.com/doi/10.1080/22221751.2019.1590128 |
| _version_ | 1797385803704303616 |
|---|---|
| author | Dongming Zhao Renqiang Liu Xianfeng Zhang Fang Li Jingfei Wang Jiwen Zhang Xing Liu Lulu Wang Jiaoer Zhang Xinzhou Wu Yuntao Guan Weiye Chen Xijun Wang Xijun He Zhigao Bu |
| author_facet | Dongming Zhao Renqiang Liu Xianfeng Zhang Fang Li Jingfei Wang Jiwen Zhang Xing Liu Lulu Wang Jiaoer Zhang Xinzhou Wu Yuntao Guan Weiye Chen Xijun Wang Xijun He Zhigao Bu |
| author_sort | Dongming Zhao |
| collection | DOAJ |
| description | ABSTRACTAfrican swine fever (ASF) entered China in August 2018 and rapidly spread across the entire country, severely threatening the Chinese domestic pig population, which accounts for more than 50% of the pig population worldwide. In this study, an ASFV isolate, Pig/Heilongjiang/2018 (Pig/HLJ/18), was isolated in primary porcine alveolar macrophages (PAMs) from a pig sample from an ASF outbreak farm. The isolate was characterized by using the haemadsorption (HAD) test, Western blotting and immunofluorescence, and electronic microscopy. Phylogenetic analysis of the viral p72 gene revealed that Pig/HLJ/18 belongs to Genotype II. Infectious titres of virus propagated in primary PAMs and pig marrow macrophages were as high as 107.2 HAD50/ml. Specific-pathogen-free pigs intramuscularly inoculated with different virus dosages at 103.5–106.5 HAD50 showed acute disease with fever and haemorrhagic signs. The incubation periods were 3–5 days for virus-inoculated pigs and 9 days for contact pigs. All virus-inoculated pigs died between 6–9 days post-inoculation (p.i.), and the contact pigs died between 13–14 days post-contact (p.c.). Viremia started on day 2 p.i. in inoculated pigs and on day 9 p.c. in contact pigs. Viral genomic DNA started to be detected from oral and rectal swab samples on 2–5 days p.i. in virus-inoculated pigs, and 6–10 days p.c. in contact pigs. These results indicate that Pig/HLJ/18 is highly virulent and transmissible in domestic pigs. Our study demonstrates the threat of ASFV and emphasizes the need to control and eradicate ASF in China. |
| first_indexed | 2024-03-08T21:59:38Z |
| format | Article |
| id | doaj.art-f00ea9e1cdbb4f108838b59b359e9cec |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-03-08T21:59:38Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-f00ea9e1cdbb4f108838b59b359e9cec2023-12-19T16:09:57ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512019-01-018143844710.1080/22221751.2019.1590128Replication and virulence in pigs of the first African swine fever virus isolated in ChinaDongming Zhao0Renqiang Liu1Xianfeng Zhang2Fang Li3Jingfei Wang4Jiwen Zhang5Xing Liu6Lulu Wang7Jiaoer Zhang8Xinzhou Wu9Yuntao Guan10Weiye Chen11Xijun Wang12Xijun He13Zhigao Bu14State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People’s Republic of ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People’s Republic of ChinaNational High Containment Laboratory for Animal Diseases Control and Prevention, Harbin, People’s Republic of ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People’s Republic of ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People’s Republic of ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People’s Republic of ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People’s Republic of ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People’s Republic of ChinaNational High Containment Laboratory for Animal Diseases Control and Prevention, Harbin, People’s Republic of ChinaNational High Containment Laboratory for Animal Diseases Control and Prevention, Harbin, People’s Republic of ChinaNational High Containment Laboratory for Animal Diseases Control and Prevention, Harbin, People’s Republic of ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People’s Republic of ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People’s Republic of ChinaNational High Containment Laboratory for Animal Diseases Control and Prevention, Harbin, People’s Republic of ChinaState Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People’s Republic of ChinaABSTRACTAfrican swine fever (ASF) entered China in August 2018 and rapidly spread across the entire country, severely threatening the Chinese domestic pig population, which accounts for more than 50% of the pig population worldwide. In this study, an ASFV isolate, Pig/Heilongjiang/2018 (Pig/HLJ/18), was isolated in primary porcine alveolar macrophages (PAMs) from a pig sample from an ASF outbreak farm. The isolate was characterized by using the haemadsorption (HAD) test, Western blotting and immunofluorescence, and electronic microscopy. Phylogenetic analysis of the viral p72 gene revealed that Pig/HLJ/18 belongs to Genotype II. Infectious titres of virus propagated in primary PAMs and pig marrow macrophages were as high as 107.2 HAD50/ml. Specific-pathogen-free pigs intramuscularly inoculated with different virus dosages at 103.5–106.5 HAD50 showed acute disease with fever and haemorrhagic signs. The incubation periods were 3–5 days for virus-inoculated pigs and 9 days for contact pigs. All virus-inoculated pigs died between 6–9 days post-inoculation (p.i.), and the contact pigs died between 13–14 days post-contact (p.c.). Viremia started on day 2 p.i. in inoculated pigs and on day 9 p.c. in contact pigs. Viral genomic DNA started to be detected from oral and rectal swab samples on 2–5 days p.i. in virus-inoculated pigs, and 6–10 days p.c. in contact pigs. These results indicate that Pig/HLJ/18 is highly virulent and transmissible in domestic pigs. Our study demonstrates the threat of ASFV and emphasizes the need to control and eradicate ASF in China.https://www.tandfonline.com/doi/10.1080/22221751.2019.1590128African swine fever virusvirus isolationanimal studypigtransmission |
| spellingShingle | Dongming Zhao Renqiang Liu Xianfeng Zhang Fang Li Jingfei Wang Jiwen Zhang Xing Liu Lulu Wang Jiaoer Zhang Xinzhou Wu Yuntao Guan Weiye Chen Xijun Wang Xijun He Zhigao Bu Replication and virulence in pigs of the first African swine fever virus isolated in China Emerging Microbes and Infections African swine fever virus virus isolation animal study pig transmission |
| title | Replication and virulence in pigs of the first African swine fever virus isolated in China |
| title_full | Replication and virulence in pigs of the first African swine fever virus isolated in China |
| title_fullStr | Replication and virulence in pigs of the first African swine fever virus isolated in China |
| title_full_unstemmed | Replication and virulence in pigs of the first African swine fever virus isolated in China |
| title_short | Replication and virulence in pigs of the first African swine fever virus isolated in China |
| title_sort | replication and virulence in pigs of the first african swine fever virus isolated in china |
| topic | African swine fever virus virus isolation animal study pig transmission |
| url | https://www.tandfonline.com/doi/10.1080/22221751.2019.1590128 |
| work_keys_str_mv | AT dongmingzhao replicationandvirulenceinpigsofthefirstafricanswinefevervirusisolatedinchina AT renqiangliu replicationandvirulenceinpigsofthefirstafricanswinefevervirusisolatedinchina AT xianfengzhang replicationandvirulenceinpigsofthefirstafricanswinefevervirusisolatedinchina AT fangli replicationandvirulenceinpigsofthefirstafricanswinefevervirusisolatedinchina AT jingfeiwang replicationandvirulenceinpigsofthefirstafricanswinefevervirusisolatedinchina AT jiwenzhang replicationandvirulenceinpigsofthefirstafricanswinefevervirusisolatedinchina AT xingliu replicationandvirulenceinpigsofthefirstafricanswinefevervirusisolatedinchina AT luluwang replicationandvirulenceinpigsofthefirstafricanswinefevervirusisolatedinchina AT jiaoerzhang replicationandvirulenceinpigsofthefirstafricanswinefevervirusisolatedinchina AT xinzhouwu replicationandvirulenceinpigsofthefirstafricanswinefevervirusisolatedinchina AT yuntaoguan replicationandvirulenceinpigsofthefirstafricanswinefevervirusisolatedinchina AT weiyechen replicationandvirulenceinpigsofthefirstafricanswinefevervirusisolatedinchina AT xijunwang replicationandvirulenceinpigsofthefirstafricanswinefevervirusisolatedinchina AT xijunhe replicationandvirulenceinpigsofthefirstafricanswinefevervirusisolatedinchina AT zhigaobu replicationandvirulenceinpigsofthefirstafricanswinefevervirusisolatedinchina |