Genetic and chemical markers for authentication of three Artemisia species: A. capillaris, A. gmelinii, and A. fukudo.
The genus Artemisia is an important source of medicines in both traditional and modern pharmaceutics, particularly in East Asia. Despite the great benefits of herbal medicine, quality assessment methods for these medicinal herbs are lacking. The young leaves from Artemisia species are generally used...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2022-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0264576 |
| _version_ | 1818335254606249984 |
|---|---|
| author | Yun Sun Lee Sunmin Woo Jin-Kyung Kim Jee Young Park Nur Kholilatul Izzah Hyun-Seung Park Jung Hwa Kang Taek Joo Lee Sang Hyun Sung Kyo Bin Kang Tae-Jin Yang |
| author_facet | Yun Sun Lee Sunmin Woo Jin-Kyung Kim Jee Young Park Nur Kholilatul Izzah Hyun-Seung Park Jung Hwa Kang Taek Joo Lee Sang Hyun Sung Kyo Bin Kang Tae-Jin Yang |
| author_sort | Yun Sun Lee |
| collection | DOAJ |
| description | The genus Artemisia is an important source of medicines in both traditional and modern pharmaceutics, particularly in East Asia. Despite the great benefits of herbal medicine, quality assessment methods for these medicinal herbs are lacking. The young leaves from Artemisia species are generally used, and most of the species have similar morphology, which often leads to adulteration and misuse. This study assembled five complete chloroplast genomes of three Artemisia species, two accessions of A. gmelinii and A. capillaris, and one A. fukudo. Through comparative analysis, we revealed genomic variations and phylogenetic relationships between these species and developed seven InDel-based barcode markers which discriminated the tested species from each other. Additionally, we analyzed specialized metabolites from the species using LC-MS and suggested chemical markers for the identification and authentication of these herbs. We expect that this integrated and complementary authentication method would aid in reducing the misuse of Artemisia species. |
| first_indexed | 2024-12-13T14:20:30Z |
| format | Article |
| id | doaj.art-f0102c5b5a9b4feb93fa7edeb940bbed |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T14:20:30Z |
| publishDate | 2022-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f0102c5b5a9b4feb93fa7edeb940bbed2022-12-21T23:42:07ZengPublic Library of Science (PLoS)PLoS ONE1932-62032022-01-01173e026457610.1371/journal.pone.0264576Genetic and chemical markers for authentication of three Artemisia species: A. capillaris, A. gmelinii, and A. fukudo.Yun Sun LeeSunmin WooJin-Kyung KimJee Young ParkNur Kholilatul IzzahHyun-Seung ParkJung Hwa KangTaek Joo LeeSang Hyun SungKyo Bin KangTae-Jin YangThe genus Artemisia is an important source of medicines in both traditional and modern pharmaceutics, particularly in East Asia. Despite the great benefits of herbal medicine, quality assessment methods for these medicinal herbs are lacking. The young leaves from Artemisia species are generally used, and most of the species have similar morphology, which often leads to adulteration and misuse. This study assembled five complete chloroplast genomes of three Artemisia species, two accessions of A. gmelinii and A. capillaris, and one A. fukudo. Through comparative analysis, we revealed genomic variations and phylogenetic relationships between these species and developed seven InDel-based barcode markers which discriminated the tested species from each other. Additionally, we analyzed specialized metabolites from the species using LC-MS and suggested chemical markers for the identification and authentication of these herbs. We expect that this integrated and complementary authentication method would aid in reducing the misuse of Artemisia species.https://doi.org/10.1371/journal.pone.0264576 |
| spellingShingle | Yun Sun Lee Sunmin Woo Jin-Kyung Kim Jee Young Park Nur Kholilatul Izzah Hyun-Seung Park Jung Hwa Kang Taek Joo Lee Sang Hyun Sung Kyo Bin Kang Tae-Jin Yang Genetic and chemical markers for authentication of three Artemisia species: A. capillaris, A. gmelinii, and A. fukudo. PLoS ONE |
| title | Genetic and chemical markers for authentication of three Artemisia species: A. capillaris, A. gmelinii, and A. fukudo. |
| title_full | Genetic and chemical markers for authentication of three Artemisia species: A. capillaris, A. gmelinii, and A. fukudo. |
| title_fullStr | Genetic and chemical markers for authentication of three Artemisia species: A. capillaris, A. gmelinii, and A. fukudo. |
| title_full_unstemmed | Genetic and chemical markers for authentication of three Artemisia species: A. capillaris, A. gmelinii, and A. fukudo. |
| title_short | Genetic and chemical markers for authentication of three Artemisia species: A. capillaris, A. gmelinii, and A. fukudo. |
| title_sort | genetic and chemical markers for authentication of three artemisia species a capillaris a gmelinii and a fukudo |
| url | https://doi.org/10.1371/journal.pone.0264576 |
| work_keys_str_mv | AT yunsunlee geneticandchemicalmarkersforauthenticationofthreeartemisiaspeciesacapillarisagmeliniiandafukudo AT sunminwoo geneticandchemicalmarkersforauthenticationofthreeartemisiaspeciesacapillarisagmeliniiandafukudo AT jinkyungkim geneticandchemicalmarkersforauthenticationofthreeartemisiaspeciesacapillarisagmeliniiandafukudo AT jeeyoungpark geneticandchemicalmarkersforauthenticationofthreeartemisiaspeciesacapillarisagmeliniiandafukudo AT nurkholilatulizzah geneticandchemicalmarkersforauthenticationofthreeartemisiaspeciesacapillarisagmeliniiandafukudo AT hyunseungpark geneticandchemicalmarkersforauthenticationofthreeartemisiaspeciesacapillarisagmeliniiandafukudo AT junghwakang geneticandchemicalmarkersforauthenticationofthreeartemisiaspeciesacapillarisagmeliniiandafukudo AT taekjoolee geneticandchemicalmarkersforauthenticationofthreeartemisiaspeciesacapillarisagmeliniiandafukudo AT sanghyunsung geneticandchemicalmarkersforauthenticationofthreeartemisiaspeciesacapillarisagmeliniiandafukudo AT kyobinkang geneticandchemicalmarkersforauthenticationofthreeartemisiaspeciesacapillarisagmeliniiandafukudo AT taejinyang geneticandchemicalmarkersforauthenticationofthreeartemisiaspeciesacapillarisagmeliniiandafukudo |