Exploring the interactions of Ti3C2Tx, AgNPs, and Ti3C2Tx/AgNPs with human serum albumin: Insights into conformational changes and biotoxicity reduction
Ag nanoparticles (AgNPs) are toxic and can accumulate in the human body or the environment, resulting in biotoxic effects. Ti3C2Tx, a new class of graphene-like 2D nanomaterials, is used in numerous applications due to its special physicochemical properties. There is great concern about the potentia...
| Main Authors: | , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-11-01
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| Series: | Chemical Engineering Journal Advances |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666821123001242 |
| _version_ | 1797388714836492288 |
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| author | Juan Bao Tingting Zhang Shiying Wu Chunhu Tan Chang Liu Lingling Wang Weihua Li Xianhuai Huang Jiansheng Li Rui Lu |
| author_facet | Juan Bao Tingting Zhang Shiying Wu Chunhu Tan Chang Liu Lingling Wang Weihua Li Xianhuai Huang Jiansheng Li Rui Lu |
| author_sort | Juan Bao |
| collection | DOAJ |
| description | Ag nanoparticles (AgNPs) are toxic and can accumulate in the human body or the environment, resulting in biotoxic effects. Ti3C2Tx, a new class of graphene-like 2D nanomaterials, is used in numerous applications due to its special physicochemical properties. There is great concern about the potential damage that this material may cause to the environment and human health. In this study, the interaction mechanisms of Ti3C2Tx, AgNPs and Ti3C2Tx/AgNPs materials with human serum albumin (HSA) were characterized using multispectral analysis and zeta potential measurements, respectively. The results showed that the effects of the three materials on the conformational changes of HSA were AgNPs > Ti3C2Tx/AgNPs > Ti3C2Tx in order. Hydrogen bonding and electrostatic interaction forces played an important role in the binding process of Ti3C2Tx and its complexes with HSA. Ag nanoparticles have a comparatively high level of biotoxicity and have a more significant effect on the conformational changes of proteins. Ti3C2Tx itself has biocompatibility and a large specific surface area. The biotoxicity of Ti3C2Tx/AgNPs complex is lower than that of AgNPs, indicating that the addition of Ti3C2Tx can reduce the biotoxicity of Ag nanoparticles, and Ti3C2Tx may be used as a carrier substance for Ag nanoparticles, which can reduce the toxicity of AgNPs while giving it unique properties similar to those of Ti3C2Tx, thus improve the biosafety of Ag nanoparticles in the application process. |
| first_indexed | 2024-03-08T22:44:48Z |
| format | Article |
| id | doaj.art-f0141a85dcc74c0a8a10cd74c0066453 |
| institution | Directory Open Access Journal |
| issn | 2666-8211 |
| language | English |
| last_indexed | 2024-03-08T22:44:48Z |
| publishDate | 2023-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Chemical Engineering Journal Advances |
| spelling | doaj.art-f0141a85dcc74c0a8a10cd74c00664532023-12-17T06:42:21ZengElsevierChemical Engineering Journal Advances2666-82112023-11-0116100567Exploring the interactions of Ti3C2Tx, AgNPs, and Ti3C2Tx/AgNPs with human serum albumin: Insights into conformational changes and biotoxicity reductionJuan Bao0Tingting Zhang1Shiying Wu2Chunhu Tan3Chang Liu4Lingling Wang5Weihua Li6Xianhuai Huang7Jiansheng Li8Rui Lu9Jiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, ChinaJiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, ChinaJiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, ChinaJiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, ChinaInformation Materials and Intelligent Sensing Laboratory of Anhui Province, Anhui University, Hefei 230601, ChinaGuangdong Provincial Key Laboratory of Microbial Culture Collection and Application, State Key Laboratory of Applied Microbiology Southern China, Institute of Microbiology, Guangdong Academy of Sciences, Guangzhou 510070, ChinaSchool of Environment and Energy Engineering, Anhui Provincial Key Laboratory of Environmental Pollution Control and Resource Reuse, Anhui Jianzhu University, Hefei 230601, ChinaSchool of Environment and Energy Engineering, Anhui Provincial Key Laboratory of Environmental Pollution Control and Resource Reuse, Anhui Jianzhu University, Hefei 230601, ChinaJiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, ChinaJiangsu Key Laboratory of Chemical Pollution Control and Resources Reuse, School of Environmental and Biological Engineering, Nanjing University of Science and Technology, Nanjing 210094, China; Corresponding author.Ag nanoparticles (AgNPs) are toxic and can accumulate in the human body or the environment, resulting in biotoxic effects. Ti3C2Tx, a new class of graphene-like 2D nanomaterials, is used in numerous applications due to its special physicochemical properties. There is great concern about the potential damage that this material may cause to the environment and human health. In this study, the interaction mechanisms of Ti3C2Tx, AgNPs and Ti3C2Tx/AgNPs materials with human serum albumin (HSA) were characterized using multispectral analysis and zeta potential measurements, respectively. The results showed that the effects of the three materials on the conformational changes of HSA were AgNPs > Ti3C2Tx/AgNPs > Ti3C2Tx in order. Hydrogen bonding and electrostatic interaction forces played an important role in the binding process of Ti3C2Tx and its complexes with HSA. Ag nanoparticles have a comparatively high level of biotoxicity and have a more significant effect on the conformational changes of proteins. Ti3C2Tx itself has biocompatibility and a large specific surface area. The biotoxicity of Ti3C2Tx/AgNPs complex is lower than that of AgNPs, indicating that the addition of Ti3C2Tx can reduce the biotoxicity of Ag nanoparticles, and Ti3C2Tx may be used as a carrier substance for Ag nanoparticles, which can reduce the toxicity of AgNPs while giving it unique properties similar to those of Ti3C2Tx, thus improve the biosafety of Ag nanoparticles in the application process.http://www.sciencedirect.com/science/article/pii/S2666821123001242Ti3C2Tx/AgNPsFluorescence spectroscopyCircular dichroismFTIRHSA conformational changes |
| spellingShingle | Juan Bao Tingting Zhang Shiying Wu Chunhu Tan Chang Liu Lingling Wang Weihua Li Xianhuai Huang Jiansheng Li Rui Lu Exploring the interactions of Ti3C2Tx, AgNPs, and Ti3C2Tx/AgNPs with human serum albumin: Insights into conformational changes and biotoxicity reduction Chemical Engineering Journal Advances Ti3C2Tx/AgNPs Fluorescence spectroscopy Circular dichroism FTIR HSA conformational changes |
| title | Exploring the interactions of Ti3C2Tx, AgNPs, and Ti3C2Tx/AgNPs with human serum albumin: Insights into conformational changes and biotoxicity reduction |
| title_full | Exploring the interactions of Ti3C2Tx, AgNPs, and Ti3C2Tx/AgNPs with human serum albumin: Insights into conformational changes and biotoxicity reduction |
| title_fullStr | Exploring the interactions of Ti3C2Tx, AgNPs, and Ti3C2Tx/AgNPs with human serum albumin: Insights into conformational changes and biotoxicity reduction |
| title_full_unstemmed | Exploring the interactions of Ti3C2Tx, AgNPs, and Ti3C2Tx/AgNPs with human serum albumin: Insights into conformational changes and biotoxicity reduction |
| title_short | Exploring the interactions of Ti3C2Tx, AgNPs, and Ti3C2Tx/AgNPs with human serum albumin: Insights into conformational changes and biotoxicity reduction |
| title_sort | exploring the interactions of ti3c2tx agnps and ti3c2tx agnps with human serum albumin insights into conformational changes and biotoxicity reduction |
| topic | Ti3C2Tx/AgNPs Fluorescence spectroscopy Circular dichroism FTIR HSA conformational changes |
| url | http://www.sciencedirect.com/science/article/pii/S2666821123001242 |
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