Expression Profile of Glossina pallidipes MicroRNAs During Symptomatic and Asymptomatic Infection With Glossina pallidipes Salivary Gland Hypertrophy Virus (Hytrosavirus)
The Glossina pallidipes salivary gland hypertrophy virus (GpSGHV) infects tsetse flies predominantly asymptomatically and occasionally symptomatically. Symptomatic infections are characterized by overt salivary gland hypertrophy (SGH) in mass reared tsetse flies, which causes reproductive dysfunctio...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-09-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fmicb.2018.02037/full |
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| author | Irene K. Meki Irene K. Meki İkbal A. İnce İkbal A. İnce Henry M. Kariithi Henry M. Kariithi Drion G. Boucias Orhan Ozcan Andrew G. Parker Just M. Vlak Monique M. van Oers Adly M. M. Abd-Alla |
| author_facet | Irene K. Meki Irene K. Meki İkbal A. İnce İkbal A. İnce Henry M. Kariithi Henry M. Kariithi Drion G. Boucias Orhan Ozcan Andrew G. Parker Just M. Vlak Monique M. van Oers Adly M. M. Abd-Alla |
| author_sort | Irene K. Meki |
| collection | DOAJ |
| description | The Glossina pallidipes salivary gland hypertrophy virus (GpSGHV) infects tsetse flies predominantly asymptomatically and occasionally symptomatically. Symptomatic infections are characterized by overt salivary gland hypertrophy (SGH) in mass reared tsetse flies, which causes reproductive dysfunctions and colony collapse, thus hindering tsetse control via sterile insect technique (SIT). Asymptomatic infections have no apparent cost to the fly’s fitness. Here, small RNAs were sequenced and profiles in asymptomatically and symptomatically infected G. pallidipes flies determined. Thirty-eight host-encoded microRNAs (miRNAs) were present in both the asymptomatic and symptomatic fly profiles, while nine host miRNAs were expressed specifically in asymptomatic flies versus 10 in symptomatic flies. Of the shared 38 miRNAs, 15 were differentially expressed when comparing asymptomatic with symptomatic flies. The most up-regulated host miRNAs in symptomatic flies was predicted to target immune-related mRNAs of the host. Six GpSGHV-encoded miRNAs were identified, of which five of them were only in symptomatic flies. These virus-encoded miRNAs may not only target host immune genes but may also participate in viral immune evasion. This evidence of differential host miRNA profile in Glossina in symptomatic flies advances our understanding of the GpSGHV-Glossina interactions and provides potential new avenues, for instance by utilization of particular miRNA inhibitors or mimics to better manage GpSGHV infections in tsetse mass-rearing facilities, a prerequisite for successful SIT implementation. |
| first_indexed | 2024-04-12T21:59:23Z |
| format | Article |
| id | doaj.art-f0158e276813401eba63fbed0c72d600 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-12T21:59:23Z |
| publishDate | 2018-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-f0158e276813401eba63fbed0c72d6002022-12-22T03:15:11ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2018-09-01910.3389/fmicb.2018.02037383966Expression Profile of Glossina pallidipes MicroRNAs During Symptomatic and Asymptomatic Infection With Glossina pallidipes Salivary Gland Hypertrophy Virus (Hytrosavirus)Irene K. Meki0Irene K. Meki1İkbal A. İnce2İkbal A. İnce3Henry M. Kariithi4Henry M. Kariithi5Drion G. Boucias6Orhan Ozcan7Andrew G. Parker8Just M. Vlak9Monique M. van Oers10Adly M. M. Abd-Alla11Insect Pest Control Laboratory, Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Vienna, AustriaLaboratory of Virology, Wageningen University and Research, Wageningen, NetherlandsDepartment of Medical Microbiology, School of Medicine, Acıbadem Mehmet Ali Aydınlar University, Istanbul, TurkeyDepartment of Biostatistics and Medical Informatics, Acıbadem Mehmet Ali Aydınlar University, Istanbul, TurkeyInsect Pest Control Laboratory, Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Vienna, AustriaBiotechnology Research Institute, Kenya Agricultural and Livestock Research Organization, Nairobi, KenyaEntomology and Nematology Department, University of Florida, Gainesville, FL, United StatesDepartment of Biostatistics and Medical Informatics, Acıbadem Mehmet Ali Aydınlar University, Istanbul, TurkeyInsect Pest Control Laboratory, Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Vienna, AustriaLaboratory of Virology, Wageningen University and Research, Wageningen, NetherlandsLaboratory of Virology, Wageningen University and Research, Wageningen, NetherlandsInsect Pest Control Laboratory, Joint FAO/IAEA Programme of Nuclear Techniques in Food and Agriculture, International Atomic Energy Agency, Vienna, AustriaThe Glossina pallidipes salivary gland hypertrophy virus (GpSGHV) infects tsetse flies predominantly asymptomatically and occasionally symptomatically. Symptomatic infections are characterized by overt salivary gland hypertrophy (SGH) in mass reared tsetse flies, which causes reproductive dysfunctions and colony collapse, thus hindering tsetse control via sterile insect technique (SIT). Asymptomatic infections have no apparent cost to the fly’s fitness. Here, small RNAs were sequenced and profiles in asymptomatically and symptomatically infected G. pallidipes flies determined. Thirty-eight host-encoded microRNAs (miRNAs) were present in both the asymptomatic and symptomatic fly profiles, while nine host miRNAs were expressed specifically in asymptomatic flies versus 10 in symptomatic flies. Of the shared 38 miRNAs, 15 were differentially expressed when comparing asymptomatic with symptomatic flies. The most up-regulated host miRNAs in symptomatic flies was predicted to target immune-related mRNAs of the host. Six GpSGHV-encoded miRNAs were identified, of which five of them were only in symptomatic flies. These virus-encoded miRNAs may not only target host immune genes but may also participate in viral immune evasion. This evidence of differential host miRNA profile in Glossina in symptomatic flies advances our understanding of the GpSGHV-Glossina interactions and provides potential new avenues, for instance by utilization of particular miRNA inhibitors or mimics to better manage GpSGHV infections in tsetse mass-rearing facilities, a prerequisite for successful SIT implementation.https://www.frontiersin.org/article/10.3389/fmicb.2018.02037/fullHytrosaviridaeGlossinamiRNAimmune systemGpSGHVsterile insect technique |
| spellingShingle | Irene K. Meki Irene K. Meki İkbal A. İnce İkbal A. İnce Henry M. Kariithi Henry M. Kariithi Drion G. Boucias Orhan Ozcan Andrew G. Parker Just M. Vlak Monique M. van Oers Adly M. M. Abd-Alla Expression Profile of Glossina pallidipes MicroRNAs During Symptomatic and Asymptomatic Infection With Glossina pallidipes Salivary Gland Hypertrophy Virus (Hytrosavirus) Frontiers in Microbiology Hytrosaviridae Glossina miRNA immune system GpSGHV sterile insect technique |
| title | Expression Profile of Glossina pallidipes MicroRNAs During Symptomatic and Asymptomatic Infection With Glossina pallidipes Salivary Gland Hypertrophy Virus (Hytrosavirus) |
| title_full | Expression Profile of Glossina pallidipes MicroRNAs During Symptomatic and Asymptomatic Infection With Glossina pallidipes Salivary Gland Hypertrophy Virus (Hytrosavirus) |
| title_fullStr | Expression Profile of Glossina pallidipes MicroRNAs During Symptomatic and Asymptomatic Infection With Glossina pallidipes Salivary Gland Hypertrophy Virus (Hytrosavirus) |
| title_full_unstemmed | Expression Profile of Glossina pallidipes MicroRNAs During Symptomatic and Asymptomatic Infection With Glossina pallidipes Salivary Gland Hypertrophy Virus (Hytrosavirus) |
| title_short | Expression Profile of Glossina pallidipes MicroRNAs During Symptomatic and Asymptomatic Infection With Glossina pallidipes Salivary Gland Hypertrophy Virus (Hytrosavirus) |
| title_sort | expression profile of glossina pallidipes micrornas during symptomatic and asymptomatic infection with glossina pallidipes salivary gland hypertrophy virus hytrosavirus |
| topic | Hytrosaviridae Glossina miRNA immune system GpSGHV sterile insect technique |
| url | https://www.frontiersin.org/article/10.3389/fmicb.2018.02037/full |
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