Origin of the Rare Hybrid Genus ×<i>Trisetokoeleria</i> Tzvelev (<i>Poaceae</i>) According to Molecular Phylogenetic Data
In our article, we analyzed new data on the origin of the hybrid genus ×<i>Trisetokoeleria</i>. According to the morphological criteria ×<i>T. jurtzevii</i> is a hybrid between <i>Koeleria asiatica</i> s. l. and <i>Trisetum spicatum</i>, ×<i>T. t...
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MDPI AG
2022-12-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/11/24/3533 |
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| author | Alexander A. Gnutikov Nikolai N. Nosov Tatiana M. Koroleva Elizaveta O. Punina Nina S. Probatova Victoria S. Shneyer Alexander V. Rodionov |
| author_facet | Alexander A. Gnutikov Nikolai N. Nosov Tatiana M. Koroleva Elizaveta O. Punina Nina S. Probatova Victoria S. Shneyer Alexander V. Rodionov |
| author_sort | Alexander A. Gnutikov |
| collection | DOAJ |
| description | In our article, we analyzed new data on the origin of the hybrid genus ×<i>Trisetokoeleria</i>. According to the morphological criteria ×<i>T. jurtzevii</i> is a hybrid between <i>Koeleria asiatica</i> s. l. and <i>Trisetum spicatum</i>, ×<i>T. taimyrica</i>, and originated from <i>Koeleria asiatica</i> s. l. and <i>Trisetum subalpestre</i>, ×<i>T. gorodkowii</i>, a hybrid between <i>Koeleria asiatica</i> and <i>Trisetum ruprechtianum</i>. Later ×<i>T. taimyrica</i> was transferred to <i>Koeleria</i>. Parental taxa are prone to active hybridization themselves, thus, new methods of next-generation sequencing (NGS) were needed to clarify the relationships of these genera. For NGS we used the fragment 18S rDNA (part)–ITS1–5.8S rDNA (totally 441 accessions). We analyzed ITS1–5.8S rDNA–ITS2 region, <i>trn</i>L–<i>trn</i>F and <i>trn</i>K–<i>rps</i>16 from eight samples of the five species, using the Sanger method: ×<i>Trisetokoeleria jurtzevii</i>, ×<i>T. taimyrica</i>, <i>Koeleria asiatica</i>, <i>Sibirotrisetum sibiricum</i> (=<i>Trisetum sibiricum</i>), and <i>Trisetum spicatum</i>. We also studied the pollen fertility of ×<i>Trisetokoeleria</i> and its possible progenitors. Our data partly contradicted previous assumptions, based on morphological grounds, and showed us a picture of developed introgression within and between <i>Koeleria</i> and <i>Trisetum</i>. ×<i>T. jurtzevii</i>, a totally sterile hybrid formed rather recently. We can suppose that ×<i>T. jurtzevii</i> is a hybrid between <i>K. asiatica</i> and some <i>Trisetum</i> s. str. Species, but not <i>T. spicatum</i>. ×<i>T. gorodkowii</i>, a hybrid in the stage of primary stabilization; it has one unique ribotype related to <i>T. spicatum</i> s. l. The second parental species is unrelated to <i>Trisetum ruprechtianum</i>. ×<i>T. taimyrica</i> and is a stabilized hybrid species; it shares major ribotypes with the <i>T. spicatum</i>/<i>T. wrangelense</i> group and has a minor fraction of rDNA related to genus <i>Deyeuxia</i> s. l. |
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| spelling | doaj.art-f016fdab869e47439779ae415686317e2023-11-24T17:28:57ZengMDPI AGPlants2223-77472022-12-011124353310.3390/plants11243533Origin of the Rare Hybrid Genus ×<i>Trisetokoeleria</i> Tzvelev (<i>Poaceae</i>) According to Molecular Phylogenetic DataAlexander A. Gnutikov0Nikolai N. Nosov1Tatiana M. Koroleva2Elizaveta O. Punina3Nina S. Probatova4Victoria S. Shneyer5Alexander V. Rodionov6Department of Genetic Resources of Oat, Barley, Rye, Federal Research Center N. I. Vavilov All-Russian Institute of Plant Genetic Resources (VIR), 190000 St. Petersburg, RussiaLaboratory of Biosystematics and Cytology, Komarov Botanical Institute of the Russian Academy of Sciences, 197376 St. Petersburg, RussiaLaboratory of Geography and Vegetation Mapping, Komarov Botanical Institute of the Russian Academy of Sciences, 197376 St. Petersburg, RussiaLaboratory of Biosystematics and Cytology, Komarov Botanical Institute of the Russian Academy of Sciences, 197376 St. Petersburg, RussiaLaboratory of Botany, Federal Scientific Center of the East Asia Terrestrial Biodiversity, Far Eastern Branch of the Russian Academy of Sciences, 690022 Vladivostok, RussiaLaboratory of Biosystematics and Cytology, Komarov Botanical Institute of the Russian Academy of Sciences, 197376 St. Petersburg, RussiaLaboratory of Biosystematics and Cytology, Komarov Botanical Institute of the Russian Academy of Sciences, 197376 St. Petersburg, RussiaIn our article, we analyzed new data on the origin of the hybrid genus ×<i>Trisetokoeleria</i>. According to the morphological criteria ×<i>T. jurtzevii</i> is a hybrid between <i>Koeleria asiatica</i> s. l. and <i>Trisetum spicatum</i>, ×<i>T. taimyrica</i>, and originated from <i>Koeleria asiatica</i> s. l. and <i>Trisetum subalpestre</i>, ×<i>T. gorodkowii</i>, a hybrid between <i>Koeleria asiatica</i> and <i>Trisetum ruprechtianum</i>. Later ×<i>T. taimyrica</i> was transferred to <i>Koeleria</i>. Parental taxa are prone to active hybridization themselves, thus, new methods of next-generation sequencing (NGS) were needed to clarify the relationships of these genera. For NGS we used the fragment 18S rDNA (part)–ITS1–5.8S rDNA (totally 441 accessions). We analyzed ITS1–5.8S rDNA–ITS2 region, <i>trn</i>L–<i>trn</i>F and <i>trn</i>K–<i>rps</i>16 from eight samples of the five species, using the Sanger method: ×<i>Trisetokoeleria jurtzevii</i>, ×<i>T. taimyrica</i>, <i>Koeleria asiatica</i>, <i>Sibirotrisetum sibiricum</i> (=<i>Trisetum sibiricum</i>), and <i>Trisetum spicatum</i>. We also studied the pollen fertility of ×<i>Trisetokoeleria</i> and its possible progenitors. Our data partly contradicted previous assumptions, based on morphological grounds, and showed us a picture of developed introgression within and between <i>Koeleria</i> and <i>Trisetum</i>. ×<i>T. jurtzevii</i>, a totally sterile hybrid formed rather recently. We can suppose that ×<i>T. jurtzevii</i> is a hybrid between <i>K. asiatica</i> and some <i>Trisetum</i> s. str. Species, but not <i>T. spicatum</i>. ×<i>T. gorodkowii</i>, a hybrid in the stage of primary stabilization; it has one unique ribotype related to <i>T. spicatum</i> s. l. The second parental species is unrelated to <i>Trisetum ruprechtianum</i>. ×<i>T. taimyrica</i> and is a stabilized hybrid species; it shares major ribotypes with the <i>T. spicatum</i>/<i>T. wrangelense</i> group and has a minor fraction of rDNA related to genus <i>Deyeuxia</i> s. l.https://www.mdpi.com/2223-7747/11/24/3533rRNA genesgrasseshybridizationITSNGSphylogeny |
| spellingShingle | Alexander A. Gnutikov Nikolai N. Nosov Tatiana M. Koroleva Elizaveta O. Punina Nina S. Probatova Victoria S. Shneyer Alexander V. Rodionov Origin of the Rare Hybrid Genus ×<i>Trisetokoeleria</i> Tzvelev (<i>Poaceae</i>) According to Molecular Phylogenetic Data Plants rRNA genes grasses hybridization ITS NGS phylogeny |
| title | Origin of the Rare Hybrid Genus ×<i>Trisetokoeleria</i> Tzvelev (<i>Poaceae</i>) According to Molecular Phylogenetic Data |
| title_full | Origin of the Rare Hybrid Genus ×<i>Trisetokoeleria</i> Tzvelev (<i>Poaceae</i>) According to Molecular Phylogenetic Data |
| title_fullStr | Origin of the Rare Hybrid Genus ×<i>Trisetokoeleria</i> Tzvelev (<i>Poaceae</i>) According to Molecular Phylogenetic Data |
| title_full_unstemmed | Origin of the Rare Hybrid Genus ×<i>Trisetokoeleria</i> Tzvelev (<i>Poaceae</i>) According to Molecular Phylogenetic Data |
| title_short | Origin of the Rare Hybrid Genus ×<i>Trisetokoeleria</i> Tzvelev (<i>Poaceae</i>) According to Molecular Phylogenetic Data |
| title_sort | origin of the rare hybrid genus i trisetokoeleria i tzvelev i poaceae i according to molecular phylogenetic data |
| topic | rRNA genes grasses hybridization ITS NGS phylogeny |
| url | https://www.mdpi.com/2223-7747/11/24/3533 |
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