Plasma miRNA profile at COVID-19 onset predicts severity status and mortality
Background MicroRNAs (miRNAs) have a crucial role in regulating immune response against infectious diseases, showing changes early in disease onset and before the detection of the pathogen. Thus, we aimed to analyze the plasma miRNA profile at COVID-19 onset to identify miRNAs as early prognostic bi...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2022-02-01
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| Series: | Emerging Microbes and Infections |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/22221751.2022.2038021 |
| _version_ | 1828885036912345088 |
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| author | Asier Fernández-Pato Ana Virseda-Berdices Salvador Resino Pablo Ryan Oscar Martínez-González Felipe Peréz-García María Martin-Vicente Daniel Valle-Millares Oscar Brochado-Kith Rafael Blancas Amalia Martínez Francisco C. Ceballos Sofía Bartolome-Sánchez Erick Joan Vidal-Alcántara David Alonso Natalia Blanca-López Ignacio Ramirez Martinez-Acitores Laura Martin-Pedraza María Ángeles Jiménez-Sousa Amanda Fernández-Rodríguez |
| author_facet | Asier Fernández-Pato Ana Virseda-Berdices Salvador Resino Pablo Ryan Oscar Martínez-González Felipe Peréz-García María Martin-Vicente Daniel Valle-Millares Oscar Brochado-Kith Rafael Blancas Amalia Martínez Francisco C. Ceballos Sofía Bartolome-Sánchez Erick Joan Vidal-Alcántara David Alonso Natalia Blanca-López Ignacio Ramirez Martinez-Acitores Laura Martin-Pedraza María Ángeles Jiménez-Sousa Amanda Fernández-Rodríguez |
| author_sort | Asier Fernández-Pato |
| collection | DOAJ |
| description | Background MicroRNAs (miRNAs) have a crucial role in regulating immune response against infectious diseases, showing changes early in disease onset and before the detection of the pathogen. Thus, we aimed to analyze the plasma miRNA profile at COVID-19 onset to identify miRNAs as early prognostic biomarkers of severity and survival. Methods and results Plasma miRNome of 96 COVID-19 patients that developed asymptomatic/mild, moderate and severe disease was sequenced together with a group of healthy controls. Plasma immune-related biomarkers were also assessed. COVID-19 patients showed 200 significant differentially expressed (SDE) miRNAs concerning healthy controls, with upregulated putative targets of SARS-CoV-2, and inflammatory miRNAs. Among COVID-19 patients, 75 SDE miRNAs were observed in asymptomatic/mild compared to symptomatic patients, which were involved in platelet aggregation and cytokine pathways, among others. Moreover, 137 SDE miRNAs were identified between severe and moderate patients, where miRNAs targeting the SARS CoV-2 genome were the most strongly disrupted. Finally, we constructed a mortality predictive risk score (miRNA-MRS) with ten miRNAs. Patients with higher values had a higher risk of 90-days mortality (hazard ratio=4.60; p-value<0.001). Besides, the discriminant power of miRNA-MRS was significantly higher than the observed for age and gender (AUROC=0.970 vs. 0.881; p=0.042). Conclusions SARS-CoV-2 infection deeply disturbs the plasma miRNome from an early stage of COVID-19, making miRNAs highly valuable as early predictors of severity and mortality. |
| first_indexed | 2024-12-13T11:18:03Z |
| format | Article |
| id | doaj.art-f01b436ef66345b083424359a84a3209 |
| institution | Directory Open Access Journal |
| issn | 2222-1751 |
| language | English |
| last_indexed | 2024-12-13T11:18:03Z |
| publishDate | 2022-02-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Emerging Microbes and Infections |
| spelling | doaj.art-f01b436ef66345b083424359a84a32092022-12-21T23:48:34ZengTaylor & Francis GroupEmerging Microbes and Infections2222-17512022-02-010016810.1080/22221751.2022.20380212038021Plasma miRNA profile at COVID-19 onset predicts severity status and mortalityAsier Fernández-Pato0Ana Virseda-Berdices1Salvador Resino2Pablo Ryan3Oscar Martínez-González4Felipe Peréz-García5María Martin-Vicente6Daniel Valle-Millares7Oscar Brochado-Kith8Rafael Blancas9Amalia Martínez10Francisco C. Ceballos11Sofía Bartolome-Sánchez12Erick Joan Vidal-Alcántara13David Alonso14Natalia Blanca-López15Ignacio Ramirez Martinez-Acitores16Laura Martin-Pedraza17María Ángeles Jiménez-Sousa18Amanda Fernández-Rodríguez19National Center for Microbiology CNM, Health Institute Carlos III ISCIIINational Center for Microbiology CNM, Health Institute Carlos III ISCIIINational Center for Microbiology CNM, Health Institute Carlos III ISCIIIHospital Universitario Infanta LeonorHospital Universitario del TajoHospital Universitario Príncipe de AsturiasNational Center for Microbiology CNM, Health Institute Carlos III ISCIIINational Center for Microbiology CNM, Health Institute Carlos III ISCIIINational Center for Microbiology CNM, Health Institute Carlos III ISCIIIHospital Universitario del TajoHospital Universitario Infanta LeonorNational Center for Microbiology CNM, Health Institute Carlos III ISCIIINational Center for Microbiology CNM, Health Institute Carlos III ISCIIINational Center for Microbiology CNM, Health Institute Carlos III ISCIIIHospital Universitario Príncipe de AsturiasUniversity Hospital Infanta LeonorHospital Universitario del TajoHospital Universitario Infanta LeonorNational Center for Microbiology CNM, Health Institute Carlos III ISCIIINational Center for Microbiology CNM, Health Institute Carlos III ISCIIIBackground MicroRNAs (miRNAs) have a crucial role in regulating immune response against infectious diseases, showing changes early in disease onset and before the detection of the pathogen. Thus, we aimed to analyze the plasma miRNA profile at COVID-19 onset to identify miRNAs as early prognostic biomarkers of severity and survival. Methods and results Plasma miRNome of 96 COVID-19 patients that developed asymptomatic/mild, moderate and severe disease was sequenced together with a group of healthy controls. Plasma immune-related biomarkers were also assessed. COVID-19 patients showed 200 significant differentially expressed (SDE) miRNAs concerning healthy controls, with upregulated putative targets of SARS-CoV-2, and inflammatory miRNAs. Among COVID-19 patients, 75 SDE miRNAs were observed in asymptomatic/mild compared to symptomatic patients, which were involved in platelet aggregation and cytokine pathways, among others. Moreover, 137 SDE miRNAs were identified between severe and moderate patients, where miRNAs targeting the SARS CoV-2 genome were the most strongly disrupted. Finally, we constructed a mortality predictive risk score (miRNA-MRS) with ten miRNAs. Patients with higher values had a higher risk of 90-days mortality (hazard ratio=4.60; p-value<0.001). Besides, the discriminant power of miRNA-MRS was significantly higher than the observed for age and gender (AUROC=0.970 vs. 0.881; p=0.042). Conclusions SARS-CoV-2 infection deeply disturbs the plasma miRNome from an early stage of COVID-19, making miRNAs highly valuable as early predictors of severity and mortality.http://dx.doi.org/10.1080/22221751.2022.2038021sars-cov2covid-19mirnasseveritymortality |
| spellingShingle | Asier Fernández-Pato Ana Virseda-Berdices Salvador Resino Pablo Ryan Oscar Martínez-González Felipe Peréz-García María Martin-Vicente Daniel Valle-Millares Oscar Brochado-Kith Rafael Blancas Amalia Martínez Francisco C. Ceballos Sofía Bartolome-Sánchez Erick Joan Vidal-Alcántara David Alonso Natalia Blanca-López Ignacio Ramirez Martinez-Acitores Laura Martin-Pedraza María Ángeles Jiménez-Sousa Amanda Fernández-Rodríguez Plasma miRNA profile at COVID-19 onset predicts severity status and mortality Emerging Microbes and Infections sars-cov2 covid-19 mirnas severity mortality |
| title | Plasma miRNA profile at COVID-19 onset predicts severity status and mortality |
| title_full | Plasma miRNA profile at COVID-19 onset predicts severity status and mortality |
| title_fullStr | Plasma miRNA profile at COVID-19 onset predicts severity status and mortality |
| title_full_unstemmed | Plasma miRNA profile at COVID-19 onset predicts severity status and mortality |
| title_short | Plasma miRNA profile at COVID-19 onset predicts severity status and mortality |
| title_sort | plasma mirna profile at covid 19 onset predicts severity status and mortality |
| topic | sars-cov2 covid-19 mirnas severity mortality |
| url | http://dx.doi.org/10.1080/22221751.2022.2038021 |
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