Ovicidal and larvicidal potential of Rosmarinus officinalis to control gastrointestinal nematodes of sheep
Abstract Gastrointestinal Nematode Infection (GIN) are the main constraint to the production of small ruminants. Studies of medicinal plants have been an important alternative in the effort to control these parasites. Therefore, the purpose of this study was to evaluate the in vitro ovicidal and lar...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Colégio Brasileiro de Parasitologia Veterinaria
|
| Series: | Revista Brasileira de Parasitologia Veterinária |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019005015104&lng=en&tlng=en |
| _version_ | 1828758478609448960 |
|---|---|
| author | Natália Berne Pinto Leonardo Mortagua de Castro Rosária Helena Machado Azambuja Gabriela de Almeida Capella Micaele Quintana de Moura Wesley Douglas Terto Rogério Antonio Freitag Sabrina Taiza Jeske Marcos Marreiro Villela Marlete Brum Cleff Fábio Pereira Leivas Leite |
| author_facet | Natália Berne Pinto Leonardo Mortagua de Castro Rosária Helena Machado Azambuja Gabriela de Almeida Capella Micaele Quintana de Moura Wesley Douglas Terto Rogério Antonio Freitag Sabrina Taiza Jeske Marcos Marreiro Villela Marlete Brum Cleff Fábio Pereira Leivas Leite |
| author_sort | Natália Berne Pinto |
| collection | DOAJ |
| description | Abstract Gastrointestinal Nematode Infection (GIN) are the main constraint to the production of small ruminants. Studies of medicinal plants have been an important alternative in the effort to control these parasites. Therefore, the purpose of this study was to evaluate the in vitro ovicidal and larvicidal activity of essential oil of Rosmarinus officinalis. The oil was extracted, analyzed by gas chromatography and tested on GIN eggs and larvae in six concentrations, 227.5mg/mL, 113.7mg/mL, 56.8mg/mL, 28.4mg/mL, 14.2mg/mL and 7.1mg/mL. To determine the ovicidal activity, GIN eggs were recovered from sheep feces and incubated for 48h with different concentrations of the oil. For the evaluation of larval migration, third-stage larvae (L3) were obtained by fecal culture, and associated with the essential oil for 24h at the same concentrations, after which they were left for another 24 hours on microsieves, followed by the count of migrating and non-migrating larvae. The assays of R. officinalis oil showed a significant (p<0.05) 97.4% to 100% inhibition of egg hatching and a significant (p<0.05) 20% to 74% inhibition of larval migration. The main constituent revealed by gas chromatography was Eucalyptol. The results indicate that R. officinalis essential oil has ovicidal and larvicidal activity on sheep GINs. |
| first_indexed | 2024-12-11T00:42:36Z |
| format | Article |
| id | doaj.art-f01bf946dd9547ec9ec261cf4e42822d |
| institution | Directory Open Access Journal |
| issn | 1984-2961 |
| language | English |
| last_indexed | 2024-12-11T00:42:36Z |
| publisher | Colégio Brasileiro de Parasitologia Veterinaria |
| record_format | Article |
| series | Revista Brasileira de Parasitologia Veterinária |
| spelling | doaj.art-f01bf946dd9547ec9ec261cf4e42822d2022-12-22T01:26:53ZengColégio Brasileiro de Parasitologia VeterinariaRevista Brasileira de Parasitologia Veterinária1984-296110.1590/s1984-29612019060S1984-29612019005015104Ovicidal and larvicidal potential of Rosmarinus officinalis to control gastrointestinal nematodes of sheepNatália Berne PintoLeonardo Mortagua de CastroRosária Helena Machado AzambujaGabriela de Almeida CapellaMicaele Quintana de MouraWesley Douglas TertoRogério Antonio FreitagSabrina Taiza JeskeMarcos Marreiro VillelaMarlete Brum CleffFábio Pereira Leivas LeiteAbstract Gastrointestinal Nematode Infection (GIN) are the main constraint to the production of small ruminants. Studies of medicinal plants have been an important alternative in the effort to control these parasites. Therefore, the purpose of this study was to evaluate the in vitro ovicidal and larvicidal activity of essential oil of Rosmarinus officinalis. The oil was extracted, analyzed by gas chromatography and tested on GIN eggs and larvae in six concentrations, 227.5mg/mL, 113.7mg/mL, 56.8mg/mL, 28.4mg/mL, 14.2mg/mL and 7.1mg/mL. To determine the ovicidal activity, GIN eggs were recovered from sheep feces and incubated for 48h with different concentrations of the oil. For the evaluation of larval migration, third-stage larvae (L3) were obtained by fecal culture, and associated with the essential oil for 24h at the same concentrations, after which they were left for another 24 hours on microsieves, followed by the count of migrating and non-migrating larvae. The assays of R. officinalis oil showed a significant (p<0.05) 97.4% to 100% inhibition of egg hatching and a significant (p<0.05) 20% to 74% inhibition of larval migration. The main constituent revealed by gas chromatography was Eucalyptol. The results indicate that R. officinalis essential oil has ovicidal and larvicidal activity on sheep GINs.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019005015104&lng=en&tlng=enHelmintosin vitrofitoterápicosruminantes |
| spellingShingle | Natália Berne Pinto Leonardo Mortagua de Castro Rosária Helena Machado Azambuja Gabriela de Almeida Capella Micaele Quintana de Moura Wesley Douglas Terto Rogério Antonio Freitag Sabrina Taiza Jeske Marcos Marreiro Villela Marlete Brum Cleff Fábio Pereira Leivas Leite Ovicidal and larvicidal potential of Rosmarinus officinalis to control gastrointestinal nematodes of sheep Revista Brasileira de Parasitologia Veterinária Helmintos in vitro fitoterápicos ruminantes |
| title | Ovicidal and larvicidal potential of Rosmarinus officinalis to control gastrointestinal nematodes of sheep |
| title_full | Ovicidal and larvicidal potential of Rosmarinus officinalis to control gastrointestinal nematodes of sheep |
| title_fullStr | Ovicidal and larvicidal potential of Rosmarinus officinalis to control gastrointestinal nematodes of sheep |
| title_full_unstemmed | Ovicidal and larvicidal potential of Rosmarinus officinalis to control gastrointestinal nematodes of sheep |
| title_short | Ovicidal and larvicidal potential of Rosmarinus officinalis to control gastrointestinal nematodes of sheep |
| title_sort | ovicidal and larvicidal potential of rosmarinus officinalis to control gastrointestinal nematodes of sheep |
| topic | Helmintos in vitro fitoterápicos ruminantes |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-29612019005015104&lng=en&tlng=en |
| work_keys_str_mv | AT nataliabernepinto ovicidalandlarvicidalpotentialofrosmarinusofficinalistocontrolgastrointestinalnematodesofsheep AT leonardomortaguadecastro ovicidalandlarvicidalpotentialofrosmarinusofficinalistocontrolgastrointestinalnematodesofsheep AT rosariahelenamachadoazambuja ovicidalandlarvicidalpotentialofrosmarinusofficinalistocontrolgastrointestinalnematodesofsheep AT gabrieladealmeidacapella ovicidalandlarvicidalpotentialofrosmarinusofficinalistocontrolgastrointestinalnematodesofsheep AT micaelequintanademoura ovicidalandlarvicidalpotentialofrosmarinusofficinalistocontrolgastrointestinalnematodesofsheep AT wesleydouglasterto ovicidalandlarvicidalpotentialofrosmarinusofficinalistocontrolgastrointestinalnematodesofsheep AT rogerioantoniofreitag ovicidalandlarvicidalpotentialofrosmarinusofficinalistocontrolgastrointestinalnematodesofsheep AT sabrinataizajeske ovicidalandlarvicidalpotentialofrosmarinusofficinalistocontrolgastrointestinalnematodesofsheep AT marcosmarreirovillela ovicidalandlarvicidalpotentialofrosmarinusofficinalistocontrolgastrointestinalnematodesofsheep AT marletebrumcleff ovicidalandlarvicidalpotentialofrosmarinusofficinalistocontrolgastrointestinalnematodesofsheep AT fabiopereiraleivasleite ovicidalandlarvicidalpotentialofrosmarinusofficinalistocontrolgastrointestinalnematodesofsheep |