A low-cost smart system for electrophoresis-based nucleic acids detection at the visible spectrum.
Nucleic acid detection by electrophoresis is still a quick and accessible technique for many diagnosis methods, primarily at research laboratories or at the point of care units. Standard protocols detect DNA/RNA molecules through specific bound chemical dyes using a UV-transilluminator or UV-photo d...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2020-01-01
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| Series: | PLoS ONE |
| Online Access: | https://doi.org/10.1371/journal.pone.0240536 |
| _version_ | 1819128736786677760 |
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| author | Eduardo Nogueira Cunha Maria Fernanda Bezerra de Souza Daniel Carlos Ferreira Lanza João Paulo Matos Santos Lima |
| author_facet | Eduardo Nogueira Cunha Maria Fernanda Bezerra de Souza Daniel Carlos Ferreira Lanza João Paulo Matos Santos Lima |
| author_sort | Eduardo Nogueira Cunha |
| collection | DOAJ |
| description | Nucleic acid detection by electrophoresis is still a quick and accessible technique for many diagnosis methods, primarily at research laboratories or at the point of care units. Standard protocols detect DNA/RNA molecules through specific bound chemical dyes using a UV-transilluminator or UV-photo documentation system. However, the acquisition costs and availability of these devices, mainly the ones with photography and internet connection capabilities, can be prohibitive, especially in developing countries public health units. Also, ultraviolet radiation is a common additional risk factor to professionals that use electrophoresis-based nucleic acid detection. With that in mind, this work describes the development of a low-cost DNA/RNA detection smart system capable of obtaining qualitative and semi-quantitative data from gel analysis. The proposed device explores the visible light absorption range of commonly used DNA/RNA dyes using readily available parts, and simple manufacturing processes, such as light-emitting diodes (LEDs) and 3D impression. By applying IoT techniques, our system covers a wide range of color spectrum in order to detect bands from various commercially used dyes, using Bluetooth communication and a smartphone for hardware control, image capturing, and sharing. The project also enables process scalability and has low manufacturing and maintenance costs. The use of LEDs at the visible spectrum can achieve very reproducible images, providing a high potential for rapid and point-of-care diagnostics as well as applications in several fields such as healthcare, agriculture, and aquaculture. |
| first_indexed | 2024-12-22T08:32:34Z |
| format | Article |
| id | doaj.art-f01d8b15a18843ec9d88d3bd16291cc7 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-22T08:32:34Z |
| publishDate | 2020-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f01d8b15a18843ec9d88d3bd16291cc72022-12-21T18:32:28ZengPublic Library of Science (PLoS)PLoS ONE1932-62032020-01-011510e024053610.1371/journal.pone.0240536A low-cost smart system for electrophoresis-based nucleic acids detection at the visible spectrum.Eduardo Nogueira CunhaMaria Fernanda Bezerra de SouzaDaniel Carlos Ferreira LanzaJoão Paulo Matos Santos LimaNucleic acid detection by electrophoresis is still a quick and accessible technique for many diagnosis methods, primarily at research laboratories or at the point of care units. Standard protocols detect DNA/RNA molecules through specific bound chemical dyes using a UV-transilluminator or UV-photo documentation system. However, the acquisition costs and availability of these devices, mainly the ones with photography and internet connection capabilities, can be prohibitive, especially in developing countries public health units. Also, ultraviolet radiation is a common additional risk factor to professionals that use electrophoresis-based nucleic acid detection. With that in mind, this work describes the development of a low-cost DNA/RNA detection smart system capable of obtaining qualitative and semi-quantitative data from gel analysis. The proposed device explores the visible light absorption range of commonly used DNA/RNA dyes using readily available parts, and simple manufacturing processes, such as light-emitting diodes (LEDs) and 3D impression. By applying IoT techniques, our system covers a wide range of color spectrum in order to detect bands from various commercially used dyes, using Bluetooth communication and a smartphone for hardware control, image capturing, and sharing. The project also enables process scalability and has low manufacturing and maintenance costs. The use of LEDs at the visible spectrum can achieve very reproducible images, providing a high potential for rapid and point-of-care diagnostics as well as applications in several fields such as healthcare, agriculture, and aquaculture.https://doi.org/10.1371/journal.pone.0240536 |
| spellingShingle | Eduardo Nogueira Cunha Maria Fernanda Bezerra de Souza Daniel Carlos Ferreira Lanza João Paulo Matos Santos Lima A low-cost smart system for electrophoresis-based nucleic acids detection at the visible spectrum. PLoS ONE |
| title | A low-cost smart system for electrophoresis-based nucleic acids detection at the visible spectrum. |
| title_full | A low-cost smart system for electrophoresis-based nucleic acids detection at the visible spectrum. |
| title_fullStr | A low-cost smart system for electrophoresis-based nucleic acids detection at the visible spectrum. |
| title_full_unstemmed | A low-cost smart system for electrophoresis-based nucleic acids detection at the visible spectrum. |
| title_short | A low-cost smart system for electrophoresis-based nucleic acids detection at the visible spectrum. |
| title_sort | low cost smart system for electrophoresis based nucleic acids detection at the visible spectrum |
| url | https://doi.org/10.1371/journal.pone.0240536 |
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