Hypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditions
<h4>Objective</h4> The oxygen concentration within cancer tissue is known to be low, but is expected to increase rapidly when oxygen is supplied by angiogenesis and hematogenous metastasis, suggesting that rapid increases in oxygen levels might influence cancer cell physiology. Therefore...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2021-01-01
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| Series: | PLoS ONE |
| Online Access: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8535375/?tool=EBI |
| _version_ | 1819002081367818240 |
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| author | Yuta Shinohara Jumpei Washio Yuri Kobayashi Yuki Abiko Keiichi Sasaki Nobuhiro Takahashi |
| author_facet | Yuta Shinohara Jumpei Washio Yuri Kobayashi Yuki Abiko Keiichi Sasaki Nobuhiro Takahashi |
| author_sort | Yuta Shinohara |
| collection | DOAJ |
| description | <h4>Objective</h4> The oxygen concentration within cancer tissue is known to be low, but is expected to increase rapidly when oxygen is supplied by angiogenesis and hematogenous metastasis, suggesting that rapid increases in oxygen levels might influence cancer cell physiology. Therefore, we investigated the effects of oxygen concentration fluctuations on the glucose metabolism of cancer cells. <h4>Methods</h4> The glucose metabolism of oral squamous cell carcinoma (HSC-2 and HSC-3) and normal epithelial (HaCaT) cells cultured under normoxic (21% oxygen) or hypoxic (1% oxygen) conditions was measured using a pH-stat system under normoxic or hypoxic conditions. The acidic end-products and reactive oxygen species (ROS) generated by glucose metabolism were also measured. <h4>Results</h4> Under normoxic conditions, the metabolic activity of hypoxically cultured cancer cells was significantly increased, and the production of acids other than lactate was upregulated, while the normal cells did not respond to rapid increases in oxygen levels. ROS production was higher in normoxic conditions in all cells, especially the hypoxically cultured HSC-3 cells. <h4>Conclusions</h4> Rapid increases in oxygen levels might enhance the glucose metabolism of hypoxically cultured cancer cells by mainly activating the TCA cycle and electron transport system, which might activate cancer cells through the ATP and ROS generation. |
| first_indexed | 2024-12-20T22:59:26Z |
| format | Article |
| id | doaj.art-f02027260c4d417db4e9b8b723b8a416 |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T22:59:26Z |
| publishDate | 2021-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-f02027260c4d417db4e9b8b723b8a4162022-12-21T19:24:03ZengPublic Library of Science (PLoS)PLoS ONE1932-62032021-01-011610Hypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditionsYuta ShinoharaJumpei WashioYuri KobayashiYuki AbikoKeiichi SasakiNobuhiro Takahashi<h4>Objective</h4> The oxygen concentration within cancer tissue is known to be low, but is expected to increase rapidly when oxygen is supplied by angiogenesis and hematogenous metastasis, suggesting that rapid increases in oxygen levels might influence cancer cell physiology. Therefore, we investigated the effects of oxygen concentration fluctuations on the glucose metabolism of cancer cells. <h4>Methods</h4> The glucose metabolism of oral squamous cell carcinoma (HSC-2 and HSC-3) and normal epithelial (HaCaT) cells cultured under normoxic (21% oxygen) or hypoxic (1% oxygen) conditions was measured using a pH-stat system under normoxic or hypoxic conditions. The acidic end-products and reactive oxygen species (ROS) generated by glucose metabolism were also measured. <h4>Results</h4> Under normoxic conditions, the metabolic activity of hypoxically cultured cancer cells was significantly increased, and the production of acids other than lactate was upregulated, while the normal cells did not respond to rapid increases in oxygen levels. ROS production was higher in normoxic conditions in all cells, especially the hypoxically cultured HSC-3 cells. <h4>Conclusions</h4> Rapid increases in oxygen levels might enhance the glucose metabolism of hypoxically cultured cancer cells by mainly activating the TCA cycle and electron transport system, which might activate cancer cells through the ATP and ROS generation.https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8535375/?tool=EBI |
| spellingShingle | Yuta Shinohara Jumpei Washio Yuri Kobayashi Yuki Abiko Keiichi Sasaki Nobuhiro Takahashi Hypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditions PLoS ONE |
| title | Hypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditions |
| title_full | Hypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditions |
| title_fullStr | Hypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditions |
| title_full_unstemmed | Hypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditions |
| title_short | Hypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditions |
| title_sort | hypoxically cultured cells of oral squamous cell carcinoma increased their glucose metabolic activity under normoxic conditions |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8535375/?tool=EBI |
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