Water-mediated green synthesis of PbS quantum dot and its glutathione and biotin conjugates for non-invasive live cell imaging
This study addresses the cellular uptake of nanomaterials in the field of bio-applications. In the present study, we have synthesized water-soluble lead sulfide quantum dot (PbS QD) with glutathione and 3-MPA (mercaptopropionic acid) as the stabilizing ligand using a green approach. 3-MPA-capped QDs...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
The Royal Society
2018-01-01
|
| Series: | Royal Society Open Science |
| Subjects: | |
| Online Access: | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.171614 |
| _version_ | 1819198943443025920 |
|---|---|
| author | M. Vijaya Bharathi Santanu Maiti Bidisha Sarkar Kaustab Ghosh Priyankar Paira |
| author_facet | M. Vijaya Bharathi Santanu Maiti Bidisha Sarkar Kaustab Ghosh Priyankar Paira |
| author_sort | M. Vijaya Bharathi |
| collection | DOAJ |
| description | This study addresses the cellular uptake of nanomaterials in the field of bio-applications. In the present study, we have synthesized water-soluble lead sulfide quantum dot (PbS QD) with glutathione and 3-MPA (mercaptopropionic acid) as the stabilizing ligand using a green approach. 3-MPA-capped QDs were further modified with streptavidin and then bound to biotin because of its high conjugation efficiency. Labelling and bio-imaging of cells with these bio-conjugated QDs were evaluated. The bright red fluorescence from these types of QDs in HeLa cells makes these materials suitable for deep tissue imaging. |
| first_indexed | 2024-12-23T03:08:28Z |
| format | Article |
| id | doaj.art-f020e703b7c84278bcd6330b34b2fd18 |
| institution | Directory Open Access Journal |
| issn | 2054-5703 |
| language | English |
| last_indexed | 2024-12-23T03:08:28Z |
| publishDate | 2018-01-01 |
| publisher | The Royal Society |
| record_format | Article |
| series | Royal Society Open Science |
| spelling | doaj.art-f020e703b7c84278bcd6330b34b2fd182022-12-21T18:02:17ZengThe Royal SocietyRoyal Society Open Science2054-57032018-01-015310.1098/rsos.171614171614Water-mediated green synthesis of PbS quantum dot and its glutathione and biotin conjugates for non-invasive live cell imagingM. Vijaya BharathiSantanu MaitiBidisha SarkarKaustab GhoshPriyankar PairaThis study addresses the cellular uptake of nanomaterials in the field of bio-applications. In the present study, we have synthesized water-soluble lead sulfide quantum dot (PbS QD) with glutathione and 3-MPA (mercaptopropionic acid) as the stabilizing ligand using a green approach. 3-MPA-capped QDs were further modified with streptavidin and then bound to biotin because of its high conjugation efficiency. Labelling and bio-imaging of cells with these bio-conjugated QDs were evaluated. The bright red fluorescence from these types of QDs in HeLa cells makes these materials suitable for deep tissue imaging.https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.171614pbs quantum dotlive cell imaginggreen synthesisglutathionebiotinfluorescence microscopy |
| spellingShingle | M. Vijaya Bharathi Santanu Maiti Bidisha Sarkar Kaustab Ghosh Priyankar Paira Water-mediated green synthesis of PbS quantum dot and its glutathione and biotin conjugates for non-invasive live cell imaging Royal Society Open Science pbs quantum dot live cell imaging green synthesis glutathione biotin fluorescence microscopy |
| title | Water-mediated green synthesis of PbS quantum dot and its glutathione and biotin conjugates for non-invasive live cell imaging |
| title_full | Water-mediated green synthesis of PbS quantum dot and its glutathione and biotin conjugates for non-invasive live cell imaging |
| title_fullStr | Water-mediated green synthesis of PbS quantum dot and its glutathione and biotin conjugates for non-invasive live cell imaging |
| title_full_unstemmed | Water-mediated green synthesis of PbS quantum dot and its glutathione and biotin conjugates for non-invasive live cell imaging |
| title_short | Water-mediated green synthesis of PbS quantum dot and its glutathione and biotin conjugates for non-invasive live cell imaging |
| title_sort | water mediated green synthesis of pbs quantum dot and its glutathione and biotin conjugates for non invasive live cell imaging |
| topic | pbs quantum dot live cell imaging green synthesis glutathione biotin fluorescence microscopy |
| url | https://royalsocietypublishing.org/doi/pdf/10.1098/rsos.171614 |
| work_keys_str_mv | AT mvijayabharathi watermediatedgreensynthesisofpbsquantumdotanditsglutathioneandbiotinconjugatesfornoninvasivelivecellimaging AT santanumaiti watermediatedgreensynthesisofpbsquantumdotanditsglutathioneandbiotinconjugatesfornoninvasivelivecellimaging AT bidishasarkar watermediatedgreensynthesisofpbsquantumdotanditsglutathioneandbiotinconjugatesfornoninvasivelivecellimaging AT kaustabghosh watermediatedgreensynthesisofpbsquantumdotanditsglutathioneandbiotinconjugatesfornoninvasivelivecellimaging AT priyankarpaira watermediatedgreensynthesisofpbsquantumdotanditsglutathioneandbiotinconjugatesfornoninvasivelivecellimaging |