TMT-Based Quantitative Proteomic Analysis of Intestinal Organoids Infected by <i>Listeria monocytogenes</i> Strains with Different Virulence
<i>L. monocytogenes</i>, consisting of 13 serotypes, is an opportunistic food-borne pathogen that causes different host reactions depending on its serotypes. In this study, highly toxic <i>L. monocytogenes</i> <i>10403s</i> resulted in more severe infections and l...
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-06-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/23/11/6231 |
| Summary: | <i>L. monocytogenes</i>, consisting of 13 serotypes, is an opportunistic food-borne pathogen that causes different host reactions depending on its serotypes. In this study, highly toxic <i>L. monocytogenes</i> <i>10403s</i> resulted in more severe infections and lower survival rates. Additionally, to investigate the remodeling of the host proteome by strains exhibiting differential toxicity, the cellular protein responses of intestinal organoids were analyzed using tandem mass tag (TMT) labeling and high-performance liquid chromatography–mass spectrometry. The virulent strain <i>10403s</i> caused 102 up-regulated and 52 down-regulated proteins, while the low virulent strain <i>M7</i> caused 188 up-regulated and 25 down-regulated proteins. Based on the analysis of gene ontology (GO) and KEGG databases, the expressions of differential proteins in organoids infected by <i>L. monocytogenes</i> <i>10403s</i> (virulent strain) or <i>M7</i> (low virulent strain) were involved in regulating essential processes such as the biological metabolism, the energy metabolism, and immune system processes. The results showed that the immune system process, as the primary host defense response to <i>L. monocytogenes</i>, comprised five pathways, including ECM–receptor interaction, the complement and coagulation cascades, HIF-1, ferroptosis, and NOD-like receptor signaling pathways. As for the <i>L. monocytogenes</i> <i>10403s</i> vs. <i>M7</i> group, the expression of differential proteins was involved in two pathways: systemic lupus erythematosus and transcriptional mis-regulation in cancer. All in all, these results revealed that <i>L. monocytogenes</i> strains with different toxicity induced similar biological functions and immune responses while having different regulations on differential proteins in the pathway. |
|---|---|
| ISSN: | 1661-6596 1422-0067 |