Effect of thermal manipulation during embryogenesis on gene expression of myogenic upstream activation factors pre- and post-hatch in broilers
This study aimed to determine the optimum timing of embryonic thermal manipulation (TM) that may result in improvement of mRNA expression of myogenic upstream activation factors viz. Wnt family member-3 (Wnt-3), sonic hedgehog gene (Shh), proliferating cell nuclear antigen (PCNA) and paired-box tra...
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| Format: | Article |
| Language: | English |
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Indian Council of Agricultural Research
2023-05-01
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| Series: | Indian Journal of Animal Sciences |
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| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/126816 |
| _version_ | 1797833526306930688 |
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| author | ABDELHAFEED DALAB ABDELHAY ALI THNIAN AL-THNIAN SAEED Y AL-RAMADAN KHALID ALKHODAIR |
| author_facet | ABDELHAFEED DALAB ABDELHAY ALI THNIAN AL-THNIAN SAEED Y AL-RAMADAN KHALID ALKHODAIR |
| author_sort | ABDELHAFEED DALAB |
| collection | DOAJ |
| description |
This study aimed to determine the optimum timing of embryonic thermal manipulation (TM) that may result in improvement of mRNA expression of myogenic upstream activation factors viz. Wnt family member-3 (Wnt-3), sonic hedgehog gene (Shh), proliferating cell nuclear antigen (PCNA) and paired-box transcription factor-7 (Pax-7) during development and histogenesis of broiler muscle. Fertile eggs (1440) were divided randomly and equally into 5 treatment groups including control (no TM) and four treatment groups (TM1, TM2 TM3 and TM4) that were daily subjected to 39 ̊C for 18 h with 65% relative humidity during embryonic days ED 7–11, ED 11–15, ED 15–18 and ED 7–18 respectively. Pectoral and thigh muscle mRNA expressions of myogenic upstream activation factors were evaluated by semi-quantitative real-time RT-PCR. Out of TM conditions that were investigated, TM1 resulted in a significant improvement of Wnt-3, Shh, PCNA and Pax-7 expressions in broiler pectoral and thigh muscles during embryonic and post-hatch life when compared to the control. Thus, thermal manipulation during early embryongenesis (embryonic days ED 7-11) enhance broiler skeletal muscle myoblast proliferation by triggering and inducing transcription factors that regulates myogenesis and subsequently may lead to improve cell number and size of skeletal muscle. The outcome of this study indicates that TM during ED7-11 improved muscle response to heat stress, was safe to the pectoral and thigh muscles and this method may enhance myogenesis and muscle growth in a positive manner.
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| first_indexed | 2024-04-09T14:24:29Z |
| format | Article |
| id | doaj.art-f025486766524f6a9edcea34646a6fa0 |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-04-09T14:24:29Z |
| publishDate | 2023-05-01 |
| publisher | Indian Council of Agricultural Research |
| record_format | Article |
| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-f025486766524f6a9edcea34646a6fa02023-05-04T10:34:07ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272023-05-01930410.56093/ijans.v93i04.126816Effect of thermal manipulation during embryogenesis on gene expression of myogenic upstream activation factors pre- and post-hatch in broilersABDELHAFEED DALAB0ABDELHAY ALI1THNIAN AL-THNIAN2SAEED Y AL-RAMADAN3KHALID ALKHODAIR4College of Agriculture and Veterinary Medicine, An-Najah National University. PO. Box 7 Nablus, West Bank, PalestineCollege of Veterinary Medicine, King Faisal University, P.O. Box 400, Al-Ahsa, Saudi ArabiaKing Faisal University, Al-Hassa 31982, Saudi ArabiaKing Faisal University, Al-Hassa 31982, Saudi ArabiaKing Faisal University, Al-Hassa 31982, Saudi Arabia This study aimed to determine the optimum timing of embryonic thermal manipulation (TM) that may result in improvement of mRNA expression of myogenic upstream activation factors viz. Wnt family member-3 (Wnt-3), sonic hedgehog gene (Shh), proliferating cell nuclear antigen (PCNA) and paired-box transcription factor-7 (Pax-7) during development and histogenesis of broiler muscle. Fertile eggs (1440) were divided randomly and equally into 5 treatment groups including control (no TM) and four treatment groups (TM1, TM2 TM3 and TM4) that were daily subjected to 39 ̊C for 18 h with 65% relative humidity during embryonic days ED 7–11, ED 11–15, ED 15–18 and ED 7–18 respectively. Pectoral and thigh muscle mRNA expressions of myogenic upstream activation factors were evaluated by semi-quantitative real-time RT-PCR. Out of TM conditions that were investigated, TM1 resulted in a significant improvement of Wnt-3, Shh, PCNA and Pax-7 expressions in broiler pectoral and thigh muscles during embryonic and post-hatch life when compared to the control. Thus, thermal manipulation during early embryongenesis (embryonic days ED 7-11) enhance broiler skeletal muscle myoblast proliferation by triggering and inducing transcription factors that regulates myogenesis and subsequently may lead to improve cell number and size of skeletal muscle. The outcome of this study indicates that TM during ED7-11 improved muscle response to heat stress, was safe to the pectoral and thigh muscles and this method may enhance myogenesis and muscle growth in a positive manner. https://epubs.icar.org.in/index.php/IJAnS/article/view/126816Broiler, Myogenesis, Paired-box transcription factor-7, Sonic hedgehog, Thermal Manipulation, Wnt family member-3 |
| spellingShingle | ABDELHAFEED DALAB ABDELHAY ALI THNIAN AL-THNIAN SAEED Y AL-RAMADAN KHALID ALKHODAIR Effect of thermal manipulation during embryogenesis on gene expression of myogenic upstream activation factors pre- and post-hatch in broilers Indian Journal of Animal Sciences Broiler, Myogenesis, Paired-box transcription factor-7, Sonic hedgehog, Thermal Manipulation, Wnt family member-3 |
| title | Effect of thermal manipulation during embryogenesis on gene expression of myogenic upstream activation factors pre- and post-hatch in broilers |
| title_full | Effect of thermal manipulation during embryogenesis on gene expression of myogenic upstream activation factors pre- and post-hatch in broilers |
| title_fullStr | Effect of thermal manipulation during embryogenesis on gene expression of myogenic upstream activation factors pre- and post-hatch in broilers |
| title_full_unstemmed | Effect of thermal manipulation during embryogenesis on gene expression of myogenic upstream activation factors pre- and post-hatch in broilers |
| title_short | Effect of thermal manipulation during embryogenesis on gene expression of myogenic upstream activation factors pre- and post-hatch in broilers |
| title_sort | effect of thermal manipulation during embryogenesis on gene expression of myogenic upstream activation factors pre and post hatch in broilers |
| topic | Broiler, Myogenesis, Paired-box transcription factor-7, Sonic hedgehog, Thermal Manipulation, Wnt family member-3 |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/126816 |
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