MicroRNA‐200c overexpression in cancer‐associated fibroblasts decreases interleukin‐2 secretion
Abstract miR‐200c‐3p is demonstrated to play the role of tumour suppressor in different tumours. However, the miR‐200c‐3p biological function in normal fibroblast (NF) and cancer‐associated fibroblast (CAF) remains unclear. This investigation aims to study the regulatory role of miR‐200c‐3p in the s...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-06-01
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| Series: | Clinical and Translational Discovery |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ctd2.90 |
| _version_ | 1797945505544667136 |
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| author | Laleh Shariati Golnaz Vaseghi Nazanin Vaziri Nasim Shenavar Ali Zarrabi Shaghayegh Haghjooy Javanmard |
| author_facet | Laleh Shariati Golnaz Vaseghi Nazanin Vaziri Nasim Shenavar Ali Zarrabi Shaghayegh Haghjooy Javanmard |
| author_sort | Laleh Shariati |
| collection | DOAJ |
| description | Abstract miR‐200c‐3p is demonstrated to play the role of tumour suppressor in different tumours. However, the miR‐200c‐3p biological function in normal fibroblast (NF) and cancer‐associated fibroblast (CAF) remains unclear. This investigation aims to study the regulatory role of miR‐200c‐3p in the secretion of Interleukin‐2 (IL‐2) in CAF and NF. CAFs and NFs were isolated from tumour and normal tissue specimens respectively. Immunocytochemistry was used to confirm the presence of a fibroblast specific marker, alpha‐actin smooth muscle, in NFs and CAFs. NF and CAF were transfected with scramble and miR‐200c‐3p utilizing the lipofectamine 2000 reagent. The protein levels of IL‐2 were measured in CAFs, NFs, and transfected groups with miR‐200c‐3p and scrambled using an IL‐2 enzyme‐linked immunoassay kit. miR‐200c decreased secretion of IL‐2 in transfected CAF and NF compared to controls. Results elucidated that transfection of MiR‐200c‐3p can decrease the IL‐2 secretion and consequently reduce IL‐induced tumourigenic manner in the CAF. |
| first_indexed | 2024-04-10T20:56:11Z |
| format | Article |
| id | doaj.art-f02675c4f8784a358714cb826122269b |
| institution | Directory Open Access Journal |
| issn | 2768-0622 |
| language | English |
| last_indexed | 2024-04-10T20:56:11Z |
| publishDate | 2022-06-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Translational Discovery |
| spelling | doaj.art-f02675c4f8784a358714cb826122269b2023-01-23T05:21:29ZengWileyClinical and Translational Discovery2768-06222022-06-0122n/an/a10.1002/ctd2.90MicroRNA‐200c overexpression in cancer‐associated fibroblasts decreases interleukin‐2 secretionLaleh Shariati0Golnaz Vaseghi1Nazanin Vaziri2Nasim Shenavar3Ali Zarrabi4Shaghayegh Haghjooy Javanmard5Biosensor Research Center, School of Advanced Technologies in Medicine Isfahan University of Medical Sciences Isfahan IranIsfahan Cardiovascular Research Center, Isfahan Cardiovascular Research Institute Isfahan University of Medical Sciences Isfahan IranDepartment of Medical Genetics, Cumming School of Medicine University of Calgary Calgary CanadaApplied Physiology Research Center, Isfahan Cardiovascular Research Institute Isfahan University of Medical Sciences Isfahan IranDepartment of Biomedical Engineering, Faculty of Engineering and Natural Sciences Istinye University Sariyer TurkeyApplied Physiology Research Center, Isfahan Cardiovascular Research Institute Isfahan University of Medical Sciences Isfahan IranAbstract miR‐200c‐3p is demonstrated to play the role of tumour suppressor in different tumours. However, the miR‐200c‐3p biological function in normal fibroblast (NF) and cancer‐associated fibroblast (CAF) remains unclear. This investigation aims to study the regulatory role of miR‐200c‐3p in the secretion of Interleukin‐2 (IL‐2) in CAF and NF. CAFs and NFs were isolated from tumour and normal tissue specimens respectively. Immunocytochemistry was used to confirm the presence of a fibroblast specific marker, alpha‐actin smooth muscle, in NFs and CAFs. NF and CAF were transfected with scramble and miR‐200c‐3p utilizing the lipofectamine 2000 reagent. The protein levels of IL‐2 were measured in CAFs, NFs, and transfected groups with miR‐200c‐3p and scrambled using an IL‐2 enzyme‐linked immunoassay kit. miR‐200c decreased secretion of IL‐2 in transfected CAF and NF compared to controls. Results elucidated that transfection of MiR‐200c‐3p can decrease the IL‐2 secretion and consequently reduce IL‐induced tumourigenic manner in the CAF.https://doi.org/10.1002/ctd2.90Breast cancercancer‐associated fibroblastmiR‐200c‐3pNormal fibroblast |
| spellingShingle | Laleh Shariati Golnaz Vaseghi Nazanin Vaziri Nasim Shenavar Ali Zarrabi Shaghayegh Haghjooy Javanmard MicroRNA‐200c overexpression in cancer‐associated fibroblasts decreases interleukin‐2 secretion Clinical and Translational Discovery Breast cancer cancer‐associated fibroblast miR‐200c‐3p Normal fibroblast |
| title | MicroRNA‐200c overexpression in cancer‐associated fibroblasts decreases interleukin‐2 secretion |
| title_full | MicroRNA‐200c overexpression in cancer‐associated fibroblasts decreases interleukin‐2 secretion |
| title_fullStr | MicroRNA‐200c overexpression in cancer‐associated fibroblasts decreases interleukin‐2 secretion |
| title_full_unstemmed | MicroRNA‐200c overexpression in cancer‐associated fibroblasts decreases interleukin‐2 secretion |
| title_short | MicroRNA‐200c overexpression in cancer‐associated fibroblasts decreases interleukin‐2 secretion |
| title_sort | microrna 200c overexpression in cancer associated fibroblasts decreases interleukin 2 secretion |
| topic | Breast cancer cancer‐associated fibroblast miR‐200c‐3p Normal fibroblast |
| url | https://doi.org/10.1002/ctd2.90 |
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