Features and Advantages of the Recurrent Approximation of Retention Times in Reversed-Phase High-Performance Liquid Chromatography
<b>Background</b>: Recurrent approximation of retention parameters in reversed-phase HPLC is effective for revealing anomalies that are otherwise difficult to detect, namely, the reversible hydration of analytes. This was demonstrated previously for restricted sets of analytes with aceto...
| Main Authors: | , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2024-02-01
|
| Series: | Separations |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2297-8739/11/3/69 |
| _version_ | 1827305016390582272 |
|---|---|
| author | Igor G. Zenkevich Abdennour Derouiche |
| author_facet | Igor G. Zenkevich Abdennour Derouiche |
| author_sort | Igor G. Zenkevich |
| collection | DOAJ |
| description | <b>Background</b>: Recurrent approximation of retention parameters in reversed-phase HPLC is effective for revealing anomalies that are otherwise difficult to detect, namely, the reversible hydration of analytes. This was demonstrated previously for restricted sets of analytes with acetonitrile–water eluents. Expanding the number of analytes and eluents seems to be a topical problem. Two kinds of derivatives of aromatic carbonyl compounds were characterized: unsubstituted hydrazones and oximes. <b>Methods</b>: If analyte demonstrates no anomalies in dependences of retention times vs. concentration of organic modifier, the recurrent approximations of these dependences are linear. To explain the features of recurrent approximations, the numerical experiments were proposed and considered. The artificial shifting of one, two, or more points allows for the modeling the different kinds of deviations of approximations from linearity. <b>Results</b>: It was shown that hydrazones are the class of analytes having no anomalies of retention parameters. On the contrary, several anomalies were detected for oximes. Downward deviations of points in the plots of recurrent approximations of retention times are the signs of reversible hydration. This effect for methanol–water eluents was detected for the first time. Some of oximes underwent hydrolysis. <b>Conclusions</b>: Recurrent approximation of retention times allows detecting chemical transformations of analytes during RP HPLC analysis. |
| first_indexed | 2024-04-24T17:50:02Z |
| format | Article |
| id | doaj.art-f0326d08c2344c2d8661163cc25024c5 |
| institution | Directory Open Access Journal |
| issn | 2297-8739 |
| language | English |
| last_indexed | 2024-04-24T17:50:02Z |
| publishDate | 2024-02-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Separations |
| spelling | doaj.art-f0326d08c2344c2d8661163cc25024c52024-03-27T14:04:25ZengMDPI AGSeparations2297-87392024-02-011136910.3390/separations11030069Features and Advantages of the Recurrent Approximation of Retention Times in Reversed-Phase High-Performance Liquid ChromatographyIgor G. Zenkevich0Abdennour Derouiche1Institute for Chemistry, St. Petersburg State University, UniversitetskyAve., 26, St. Petersburg 198504, RussiaInstitute for Chemistry, St. Petersburg State University, UniversitetskyAve., 26, St. Petersburg 198504, Russia<b>Background</b>: Recurrent approximation of retention parameters in reversed-phase HPLC is effective for revealing anomalies that are otherwise difficult to detect, namely, the reversible hydration of analytes. This was demonstrated previously for restricted sets of analytes with acetonitrile–water eluents. Expanding the number of analytes and eluents seems to be a topical problem. Two kinds of derivatives of aromatic carbonyl compounds were characterized: unsubstituted hydrazones and oximes. <b>Methods</b>: If analyte demonstrates no anomalies in dependences of retention times vs. concentration of organic modifier, the recurrent approximations of these dependences are linear. To explain the features of recurrent approximations, the numerical experiments were proposed and considered. The artificial shifting of one, two, or more points allows for the modeling the different kinds of deviations of approximations from linearity. <b>Results</b>: It was shown that hydrazones are the class of analytes having no anomalies of retention parameters. On the contrary, several anomalies were detected for oximes. Downward deviations of points in the plots of recurrent approximations of retention times are the signs of reversible hydration. This effect for methanol–water eluents was detected for the first time. Some of oximes underwent hydrolysis. <b>Conclusions</b>: Recurrent approximation of retention times allows detecting chemical transformations of analytes during RP HPLC analysis.https://www.mdpi.com/2297-8739/11/3/69reversed-phase HPLCretention timesrecurrent approximationdetection of anomalieschemical transformations of analytes |
| spellingShingle | Igor G. Zenkevich Abdennour Derouiche Features and Advantages of the Recurrent Approximation of Retention Times in Reversed-Phase High-Performance Liquid Chromatography Separations reversed-phase HPLC retention times recurrent approximation detection of anomalies chemical transformations of analytes |
| title | Features and Advantages of the Recurrent Approximation of Retention Times in Reversed-Phase High-Performance Liquid Chromatography |
| title_full | Features and Advantages of the Recurrent Approximation of Retention Times in Reversed-Phase High-Performance Liquid Chromatography |
| title_fullStr | Features and Advantages of the Recurrent Approximation of Retention Times in Reversed-Phase High-Performance Liquid Chromatography |
| title_full_unstemmed | Features and Advantages of the Recurrent Approximation of Retention Times in Reversed-Phase High-Performance Liquid Chromatography |
| title_short | Features and Advantages of the Recurrent Approximation of Retention Times in Reversed-Phase High-Performance Liquid Chromatography |
| title_sort | features and advantages of the recurrent approximation of retention times in reversed phase high performance liquid chromatography |
| topic | reversed-phase HPLC retention times recurrent approximation detection of anomalies chemical transformations of analytes |
| url | https://www.mdpi.com/2297-8739/11/3/69 |
| work_keys_str_mv | AT igorgzenkevich featuresandadvantagesoftherecurrentapproximationofretentiontimesinreversedphasehighperformanceliquidchromatography AT abdennourderouiche featuresandadvantagesoftherecurrentapproximationofretentiontimesinreversedphasehighperformanceliquidchromatography |