The thermal stability of yellow fever vaccines
The assessment of yellow fever vaccine thermostability both in lyophilized form and after reconstitution were analyzed. Two commercial yellow fever vaccines were assayed for their thermal stability. Vaccines were exposed to test temperatures in the range of 8 (graus) C to 45 (graus) C. Residual infe...
| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Fundação Oswaldo Cruz (FIOCRUZ)
1990-09-01
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| Series: | Memorias do Instituto Oswaldo Cruz |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000300011 |
| _version_ | 1797756539301265408 |
|---|---|
| author | Ricardo Ishak Colin R. Howard |
| author_facet | Ricardo Ishak Colin R. Howard |
| author_sort | Ricardo Ishak |
| collection | DOAJ |
| description | The assessment of yellow fever vaccine thermostability both in lyophilized form and after reconstitution were analyzed. Two commercial yellow fever vaccines were assayed for their thermal stability. Vaccines were exposed to test temperatures in the range of 8 (graus) C to 45 (graus) C. Residual infectivity was measured by a plaque assay using Vero cells. The titre values were used in an accelerated degradation test that follows the Arrhenius equation and the minimum immunizing dose was assumed to be 10 (ao cubo) particles forming unit (pfu)/dose. Some of the most relevant results include that (i) regular culture medium show the same degradation pattern of a reconstituted 17D-204 vaccine; (ii) reconstituted YF-17D-204 showed a predictable half life of more than six days if kept at 0 (graus) C; (iii) there are differences in thermostability between different products that are probably due to both presence of stabilizers in the preparation and the modernization in the vaccine production; (iv) it is important to establish a proper correlation between the mouse infectivity test and the plaque assay since the last appears to be more simple, economical, and practical for small laboratories to assess the potency of the vaccine, and (v) the accelerated degradation test appears to be the best procedure to quantify the thermostability of biological products. |
| first_indexed | 2024-03-12T18:02:58Z |
| format | Article |
| id | doaj.art-f033a82ccba649f78335fda4007abbaa |
| institution | Directory Open Access Journal |
| issn | 0074-0276 1678-8060 |
| language | English |
| last_indexed | 2024-03-12T18:02:58Z |
| publishDate | 1990-09-01 |
| publisher | Fundação Oswaldo Cruz (FIOCRUZ) |
| record_format | Article |
| series | Memorias do Instituto Oswaldo Cruz |
| spelling | doaj.art-f033a82ccba649f78335fda4007abbaa2023-08-02T09:36:19ZengFundação Oswaldo Cruz (FIOCRUZ)Memorias do Instituto Oswaldo Cruz0074-02761678-80601990-09-0185333934510.1590/S0074-02761990000300011The thermal stability of yellow fever vaccinesRicardo IshakColin R. HowardThe assessment of yellow fever vaccine thermostability both in lyophilized form and after reconstitution were analyzed. Two commercial yellow fever vaccines were assayed for their thermal stability. Vaccines were exposed to test temperatures in the range of 8 (graus) C to 45 (graus) C. Residual infectivity was measured by a plaque assay using Vero cells. The titre values were used in an accelerated degradation test that follows the Arrhenius equation and the minimum immunizing dose was assumed to be 10 (ao cubo) particles forming unit (pfu)/dose. Some of the most relevant results include that (i) regular culture medium show the same degradation pattern of a reconstituted 17D-204 vaccine; (ii) reconstituted YF-17D-204 showed a predictable half life of more than six days if kept at 0 (graus) C; (iii) there are differences in thermostability between different products that are probably due to both presence of stabilizers in the preparation and the modernization in the vaccine production; (iv) it is important to establish a proper correlation between the mouse infectivity test and the plaque assay since the last appears to be more simple, economical, and practical for small laboratories to assess the potency of the vaccine, and (v) the accelerated degradation test appears to be the best procedure to quantify the thermostability of biological products.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000300011yellow fever virusyellow fever vaccinesflaviviruses |
| spellingShingle | Ricardo Ishak Colin R. Howard The thermal stability of yellow fever vaccines Memorias do Instituto Oswaldo Cruz yellow fever virus yellow fever vaccines flaviviruses |
| title | The thermal stability of yellow fever vaccines |
| title_full | The thermal stability of yellow fever vaccines |
| title_fullStr | The thermal stability of yellow fever vaccines |
| title_full_unstemmed | The thermal stability of yellow fever vaccines |
| title_short | The thermal stability of yellow fever vaccines |
| title_sort | thermal stability of yellow fever vaccines |
| topic | yellow fever virus yellow fever vaccines flaviviruses |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761990000300011 |
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