Integrated lipidomics and network pharmacology analysis to reveal the mechanisms of berberine in the treatment of hyperlipidemia
Abstract Background Berberine (BBR), an isoquinoline alkaloid isolated from Rhizoma Coptis, is widely used in the treatment of hyperlipidemia (HLP) in China. At present, the efficacy of BBR against HLP is relatively clear, but there are few researches on its mechanism. The purpose of this study was...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2022-09-01
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| Series: | Journal of Translational Medicine |
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| Online Access: | https://doi.org/10.1186/s12967-022-03623-0 |
| _version_ | 1818064882256314368 |
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| author | Yuting Chen Kaipeng Li Han Zhao Zhangsen Hao Yuxin Yang Mingming Gao Ding Zhao |
| author_facet | Yuting Chen Kaipeng Li Han Zhao Zhangsen Hao Yuxin Yang Mingming Gao Ding Zhao |
| author_sort | Yuting Chen |
| collection | DOAJ |
| description | Abstract Background Berberine (BBR), an isoquinoline alkaloid isolated from Rhizoma Coptis, is widely used in the treatment of hyperlipidemia (HLP) in China. At present, the efficacy of BBR against HLP is relatively clear, but there are few researches on its mechanism. The purpose of this study was to evaluate the potentially beneficial role of BBR in HLP hamster models, as well as investigate its possible mechanisms and potential lipid biomarkers in combination with network pharmacology. Methods HLP hamster model was induced by high-fat diet. Hematoxylin—eosin (HE) staining was used to determine the degree of hepatic pathological injury. Liquid chromatography-mass spectrometry was used to analyze lipid metabolism profiles of liver samples, and multiple statistical analysis methods were used to screen and identify lipid biomarkers. The possible molecular mechanism was unraveled by network pharmacology. Results The results showed that 13 metabolites, including CE (16:1), HexCer (D18:1/19:0) and LPC (O-22:0) were biomarkers of BBR regulation. CHPT1, PLA2G4A, LCAT and UGCG were predicted as the lipid-linked targets of BBR against HLP, whilst glycerophospholipid and sphingolipid metabolism were the key pathways of BBR against HLP. Conclusions In summary, this study provides new insights into the protective mechanism of BBR against HLP through network pharmacology and lipidomic approaches. |
| first_indexed | 2024-12-10T14:43:03Z |
| format | Article |
| id | doaj.art-f03712132b454408911935795971be1d |
| institution | Directory Open Access Journal |
| issn | 1479-5876 |
| language | English |
| last_indexed | 2024-12-10T14:43:03Z |
| publishDate | 2022-09-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Translational Medicine |
| spelling | doaj.art-f03712132b454408911935795971be1d2022-12-22T01:44:39ZengBMCJournal of Translational Medicine1479-58762022-09-0120111610.1186/s12967-022-03623-0Integrated lipidomics and network pharmacology analysis to reveal the mechanisms of berberine in the treatment of hyperlipidemiaYuting Chen0Kaipeng Li1Han Zhao2Zhangsen Hao3Yuxin Yang4Mingming Gao5Ding Zhao6The Postdoctoral Research Station of Biology, Hebei Medical UniversityThe Department of Pharmacognosy, School of Pharmacy, Hebei Medical UniversityThe Department of Pharmacognosy, School of Pharmacy, Hebei Medical UniversityThe Department of Pharmacognosy, School of Pharmacy, Hebei Medical UniversityThe Department of Pharmacognosy, School of Pharmacy, Hebei Medical UniversityThe Laboratory of Lipid Metabolism, Institute of Basic Medicine, Hebei Medical UniversityThe Department of Pharmacognosy, School of Pharmacy, Hebei Medical UniversityAbstract Background Berberine (BBR), an isoquinoline alkaloid isolated from Rhizoma Coptis, is widely used in the treatment of hyperlipidemia (HLP) in China. At present, the efficacy of BBR against HLP is relatively clear, but there are few researches on its mechanism. The purpose of this study was to evaluate the potentially beneficial role of BBR in HLP hamster models, as well as investigate its possible mechanisms and potential lipid biomarkers in combination with network pharmacology. Methods HLP hamster model was induced by high-fat diet. Hematoxylin—eosin (HE) staining was used to determine the degree of hepatic pathological injury. Liquid chromatography-mass spectrometry was used to analyze lipid metabolism profiles of liver samples, and multiple statistical analysis methods were used to screen and identify lipid biomarkers. The possible molecular mechanism was unraveled by network pharmacology. Results The results showed that 13 metabolites, including CE (16:1), HexCer (D18:1/19:0) and LPC (O-22:0) were biomarkers of BBR regulation. CHPT1, PLA2G4A, LCAT and UGCG were predicted as the lipid-linked targets of BBR against HLP, whilst glycerophospholipid and sphingolipid metabolism were the key pathways of BBR against HLP. Conclusions In summary, this study provides new insights into the protective mechanism of BBR against HLP through network pharmacology and lipidomic approaches.https://doi.org/10.1186/s12967-022-03623-0BerberineHyperlipidemiaLipidomicsNetwork pharmacology |
| spellingShingle | Yuting Chen Kaipeng Li Han Zhao Zhangsen Hao Yuxin Yang Mingming Gao Ding Zhao Integrated lipidomics and network pharmacology analysis to reveal the mechanisms of berberine in the treatment of hyperlipidemia Journal of Translational Medicine Berberine Hyperlipidemia Lipidomics Network pharmacology |
| title | Integrated lipidomics and network pharmacology analysis to reveal the mechanisms of berberine in the treatment of hyperlipidemia |
| title_full | Integrated lipidomics and network pharmacology analysis to reveal the mechanisms of berberine in the treatment of hyperlipidemia |
| title_fullStr | Integrated lipidomics and network pharmacology analysis to reveal the mechanisms of berberine in the treatment of hyperlipidemia |
| title_full_unstemmed | Integrated lipidomics and network pharmacology analysis to reveal the mechanisms of berberine in the treatment of hyperlipidemia |
| title_short | Integrated lipidomics and network pharmacology analysis to reveal the mechanisms of berberine in the treatment of hyperlipidemia |
| title_sort | integrated lipidomics and network pharmacology analysis to reveal the mechanisms of berberine in the treatment of hyperlipidemia |
| topic | Berberine Hyperlipidemia Lipidomics Network pharmacology |
| url | https://doi.org/10.1186/s12967-022-03623-0 |
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