Investigating the Effectiveness of Exopolysaccharide-Producing Lactic Acid Bacteria in Biosorbing Lead (II), Attaching to Caco-2 Cells, and Provoking Antiinflammatory Responses
Lead is a common toxic heavy metal with harmful effects on the human body and is widely used in several industries. It can contaminate the environment by air and water emissions and can enter the human body through the respiratory tract, ingestion, or skin contact. Lead is considered as a persistent...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Journal of Food Protection |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S0362028X23067789 |
| _version_ | 1797733168637280256 |
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| author | Chieh-Sheng Yang Yong-Yu Lai Cheng-Chih Tsai |
| author_facet | Chieh-Sheng Yang Yong-Yu Lai Cheng-Chih Tsai |
| author_sort | Chieh-Sheng Yang |
| collection | DOAJ |
| description | Lead is a common toxic heavy metal with harmful effects on the human body and is widely used in several industries. It can contaminate the environment by air and water emissions and can enter the human body through the respiratory tract, ingestion, or skin contact. Lead is considered as a persistent environmental pollutant, with a half-life of 30 days in the blood, and exists in the skeletal system for decades and causes damage to other systems. Biosorption is receiving increasing attention. Due to its high efficiency and economic value in removing heavy metals from the environment, a variety of biosorption methods can be used for the removal of heavy metals. Lactic acid bacteria (LAB) strains were capable of attaching to both human skin stratum corneum HaCaT cells and human rectal cancer Caco-2 cells. NBM-04-10-001 and NBM-01-07-003 significantly reduced the secretion of IL-6 and IL-8 after coculture with HaCaT cells. In the immune response of RAW264.7 mouse macrophages, high bacterial counts reduced the concentrations of IL-6 and TNF-α in a dose-dependent manner. The results of animal experiments revealed that feeding lead solution exerted no effect on the animal’s food intake, and feeding PURE LAC NBM11 powder could effectively remove lead content in the blood. The group fed with PURE LAC NBM11 powder showed significantly less damage and lesions to liver cells. The LAB powder developed in this study has the potential to bind metals, preventing them from entering the body and protecting the host. LAB can be an ideal strain for future bioadsorption chelators. |
| first_indexed | 2024-03-12T12:24:16Z |
| format | Article |
| id | doaj.art-f03a7411a6174eda9adfa49d2ee1006e |
| institution | Directory Open Access Journal |
| issn | 0362-028X |
| language | English |
| last_indexed | 2024-03-12T12:24:16Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Food Protection |
| spelling | doaj.art-f03a7411a6174eda9adfa49d2ee1006e2023-08-30T05:49:43ZengElsevierJournal of Food Protection0362-028X2023-09-01869100106Investigating the Effectiveness of Exopolysaccharide-Producing Lactic Acid Bacteria in Biosorbing Lead (II), Attaching to Caco-2 Cells, and Provoking Antiinflammatory ResponsesChieh-Sheng Yang0Yong-Yu Lai1Cheng-Chih Tsai2Department of Food Science and Technology, HungKuang University, Shalu District, Taichung City 43302, TaiwanNative Biomedical Co., Ltd., Xinshi District, Tainan City 74442, TaiwanDepartment of Food Science and Technology, HungKuang University, Shalu District, Taichung City 43302, Taiwan; Corresponding author. Address: Department of Food Science and Technology, HungKuang University, No. 1018, Sec. 6, Taiwan Boulevard, Shalu District, Taichung City 43302, Taiwan.Lead is a common toxic heavy metal with harmful effects on the human body and is widely used in several industries. It can contaminate the environment by air and water emissions and can enter the human body through the respiratory tract, ingestion, or skin contact. Lead is considered as a persistent environmental pollutant, with a half-life of 30 days in the blood, and exists in the skeletal system for decades and causes damage to other systems. Biosorption is receiving increasing attention. Due to its high efficiency and economic value in removing heavy metals from the environment, a variety of biosorption methods can be used for the removal of heavy metals. Lactic acid bacteria (LAB) strains were capable of attaching to both human skin stratum corneum HaCaT cells and human rectal cancer Caco-2 cells. NBM-04-10-001 and NBM-01-07-003 significantly reduced the secretion of IL-6 and IL-8 after coculture with HaCaT cells. In the immune response of RAW264.7 mouse macrophages, high bacterial counts reduced the concentrations of IL-6 and TNF-α in a dose-dependent manner. The results of animal experiments revealed that feeding lead solution exerted no effect on the animal’s food intake, and feeding PURE LAC NBM11 powder could effectively remove lead content in the blood. The group fed with PURE LAC NBM11 powder showed significantly less damage and lesions to liver cells. The LAB powder developed in this study has the potential to bind metals, preventing them from entering the body and protecting the host. LAB can be an ideal strain for future bioadsorption chelators.http://www.sciencedirect.com/science/article/pii/S0362028X23067789BiosorptionCaco-2 cellsHaCaT cellsLactic acid bacteriaLeadRAW264.7 mouse macrophages |
| spellingShingle | Chieh-Sheng Yang Yong-Yu Lai Cheng-Chih Tsai Investigating the Effectiveness of Exopolysaccharide-Producing Lactic Acid Bacteria in Biosorbing Lead (II), Attaching to Caco-2 Cells, and Provoking Antiinflammatory Responses Journal of Food Protection Biosorption Caco-2 cells HaCaT cells Lactic acid bacteria Lead RAW264.7 mouse macrophages |
| title | Investigating the Effectiveness of Exopolysaccharide-Producing Lactic Acid Bacteria in Biosorbing Lead (II), Attaching to Caco-2 Cells, and Provoking Antiinflammatory Responses |
| title_full | Investigating the Effectiveness of Exopolysaccharide-Producing Lactic Acid Bacteria in Biosorbing Lead (II), Attaching to Caco-2 Cells, and Provoking Antiinflammatory Responses |
| title_fullStr | Investigating the Effectiveness of Exopolysaccharide-Producing Lactic Acid Bacteria in Biosorbing Lead (II), Attaching to Caco-2 Cells, and Provoking Antiinflammatory Responses |
| title_full_unstemmed | Investigating the Effectiveness of Exopolysaccharide-Producing Lactic Acid Bacteria in Biosorbing Lead (II), Attaching to Caco-2 Cells, and Provoking Antiinflammatory Responses |
| title_short | Investigating the Effectiveness of Exopolysaccharide-Producing Lactic Acid Bacteria in Biosorbing Lead (II), Attaching to Caco-2 Cells, and Provoking Antiinflammatory Responses |
| title_sort | investigating the effectiveness of exopolysaccharide producing lactic acid bacteria in biosorbing lead ii attaching to caco 2 cells and provoking antiinflammatory responses |
| topic | Biosorption Caco-2 cells HaCaT cells Lactic acid bacteria Lead RAW264.7 mouse macrophages |
| url | http://www.sciencedirect.com/science/article/pii/S0362028X23067789 |
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