Insulin-like Androgenic Gland Hormone Induced Sex Reversal and Molecular Pathways in <i>Macrobrachium nipponense</i>: Insights into Reproduction, Growth, and Sex Differentiation
This study investigated the potential to use double-stranded RNA insulin-like androgenic gland hormone (<i>dsIAG</i>) to induce sex reversal in <i>Macrobrachium nipponense</i> and identified the molecular mechanisms underlying crustacean reproduction and sex differentiation....
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-09-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/18/14306 |
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| author | Pengfei Cai Wenyi Zhang Sufei Jiang Yiwei Xiong Huwei Yuan Zijian Gao Xuanbing Gao Cheng Ma Yongkang Zhou Yongsheng Gong Hui Qiao Shubo Jin Hongtuo Fu |
| author_facet | Pengfei Cai Wenyi Zhang Sufei Jiang Yiwei Xiong Huwei Yuan Zijian Gao Xuanbing Gao Cheng Ma Yongkang Zhou Yongsheng Gong Hui Qiao Shubo Jin Hongtuo Fu |
| author_sort | Pengfei Cai |
| collection | DOAJ |
| description | This study investigated the potential to use double-stranded RNA insulin-like androgenic gland hormone (<i>dsIAG</i>) to induce sex reversal in <i>Macrobrachium nipponense</i> and identified the molecular mechanisms underlying crustacean reproduction and sex differentiation. The study aimed to determine whether <i>dsIAG</i> could induce sex reversal in PL30-male <i>M. nipponense</i> during a critical period. The sex-related genes were selected by performing the gonadal transcriptome analysis of normal male (dsM), normal female (dsFM), neo-female sex-reversed individuals (dsRM), and unreversed males (dsNRM). After six injections, the experiment finally resulted in a 20% production of dsRM. Histologically, dsRM ovaries developed slower than dsFM, but dsNRM spermathecae developed normally. A total of 1718, 1069, and 255 differentially expressed genes were identified through transcriptome sequencing of the gonads in three comparison groups, revealing crucial genes related to reproduction and sex differentiation, such as <i>GnRHR</i>, <i>VGR</i>, <i>SG</i>, and <i>LWS</i>. Principal Component Analysis (PCA) also distinguished dsM and dsRM very well. In addition, this study predicted that the eyestalks and the “phototransduction-fly” photoperiodic pathways of <i>M. nipponense</i> could play an important role in sex reversal. The enrichment of related pathways and growth traits in dsNRM were combined to establish that <i>IAG</i> played a significant role in reproduction, growth regulation, and metabolism. Finally, complete sex reversal may depend on specific stimuli at critical periods. Overall, this study provides valuable findings for the <i>IAG</i> regulation of sex differentiation, reproduction, and growth of <i>M. nipponense</i> in establishing a monoculture. |
| first_indexed | 2024-03-10T22:39:48Z |
| format | Article |
| id | doaj.art-f03acc9c39c440468028d3b3a4e5ea67 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T22:39:48Z |
| publishDate | 2023-09-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-f03acc9c39c440468028d3b3a4e5ea672023-11-19T11:10:50ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124181430610.3390/ijms241814306Insulin-like Androgenic Gland Hormone Induced Sex Reversal and Molecular Pathways in <i>Macrobrachium nipponense</i>: Insights into Reproduction, Growth, and Sex DifferentiationPengfei Cai0Wenyi Zhang1Sufei Jiang2Yiwei Xiong3Huwei Yuan4Zijian Gao5Xuanbing Gao6Cheng Ma7Yongkang Zhou8Yongsheng Gong9Hui Qiao10Shubo Jin11Hongtuo Fu12Wuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaKey Laboratory of Freshwater Fisheries and Germplasm Resources Utilization, Ministry of Agriculture and Rural Affairs, Freshwater Fisheries Research Center, Chinese Academy of Fishery Sciences, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaWuxi Fisheries College, Nanjing Agricultural University, Wuxi 214081, ChinaThis study investigated the potential to use double-stranded RNA insulin-like androgenic gland hormone (<i>dsIAG</i>) to induce sex reversal in <i>Macrobrachium nipponense</i> and identified the molecular mechanisms underlying crustacean reproduction and sex differentiation. The study aimed to determine whether <i>dsIAG</i> could induce sex reversal in PL30-male <i>M. nipponense</i> during a critical period. The sex-related genes were selected by performing the gonadal transcriptome analysis of normal male (dsM), normal female (dsFM), neo-female sex-reversed individuals (dsRM), and unreversed males (dsNRM). After six injections, the experiment finally resulted in a 20% production of dsRM. Histologically, dsRM ovaries developed slower than dsFM, but dsNRM spermathecae developed normally. A total of 1718, 1069, and 255 differentially expressed genes were identified through transcriptome sequencing of the gonads in three comparison groups, revealing crucial genes related to reproduction and sex differentiation, such as <i>GnRHR</i>, <i>VGR</i>, <i>SG</i>, and <i>LWS</i>. Principal Component Analysis (PCA) also distinguished dsM and dsRM very well. In addition, this study predicted that the eyestalks and the “phototransduction-fly” photoperiodic pathways of <i>M. nipponense</i> could play an important role in sex reversal. The enrichment of related pathways and growth traits in dsNRM were combined to establish that <i>IAG</i> played a significant role in reproduction, growth regulation, and metabolism. Finally, complete sex reversal may depend on specific stimuli at critical periods. Overall, this study provides valuable findings for the <i>IAG</i> regulation of sex differentiation, reproduction, and growth of <i>M. nipponense</i> in establishing a monoculture.https://www.mdpi.com/1422-0067/24/18/14306<i>Macrobrachium nipponense</i>sex reversal<i>IAG</i>testisovaryreproduction |
| spellingShingle | Pengfei Cai Wenyi Zhang Sufei Jiang Yiwei Xiong Huwei Yuan Zijian Gao Xuanbing Gao Cheng Ma Yongkang Zhou Yongsheng Gong Hui Qiao Shubo Jin Hongtuo Fu Insulin-like Androgenic Gland Hormone Induced Sex Reversal and Molecular Pathways in <i>Macrobrachium nipponense</i>: Insights into Reproduction, Growth, and Sex Differentiation International Journal of Molecular Sciences <i>Macrobrachium nipponense</i> sex reversal <i>IAG</i> testis ovary reproduction |
| title | Insulin-like Androgenic Gland Hormone Induced Sex Reversal and Molecular Pathways in <i>Macrobrachium nipponense</i>: Insights into Reproduction, Growth, and Sex Differentiation |
| title_full | Insulin-like Androgenic Gland Hormone Induced Sex Reversal and Molecular Pathways in <i>Macrobrachium nipponense</i>: Insights into Reproduction, Growth, and Sex Differentiation |
| title_fullStr | Insulin-like Androgenic Gland Hormone Induced Sex Reversal and Molecular Pathways in <i>Macrobrachium nipponense</i>: Insights into Reproduction, Growth, and Sex Differentiation |
| title_full_unstemmed | Insulin-like Androgenic Gland Hormone Induced Sex Reversal and Molecular Pathways in <i>Macrobrachium nipponense</i>: Insights into Reproduction, Growth, and Sex Differentiation |
| title_short | Insulin-like Androgenic Gland Hormone Induced Sex Reversal and Molecular Pathways in <i>Macrobrachium nipponense</i>: Insights into Reproduction, Growth, and Sex Differentiation |
| title_sort | insulin like androgenic gland hormone induced sex reversal and molecular pathways in i macrobrachium nipponense i insights into reproduction growth and sex differentiation |
| topic | <i>Macrobrachium nipponense</i> sex reversal <i>IAG</i> testis ovary reproduction |
| url | https://www.mdpi.com/1422-0067/24/18/14306 |
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